antibioticsАнтибиотики и ХимиотерапияAntibiot Khimioter = Antibiotics and Chemotherapy0235-2990ООО «Издательство ОКИ»10.37489/0235-2990-2024-69-3-4-4-13BKZWDUantibiotics-1119Research ArticleЭКСПЕРИМЕНТАЛЬНЫЕ ИССЛЕДОВАНИЯEXPERIMENTAL STUDIESИсследование резистентности к антибиотикам бактерий рода Bacillus, выделенных из Международной космической станции и больничной лабораторииStudy of Antibiotic Resistance of Bacillus Bacteria Isolated from the International Space Station and a Hospital Laboratoryhttps://orcid.org/0000-0002-8467-9051ЕникеевР. Р.YenikeyevR. R.

Еникеев Радмир Рустамович — кафедра микробиологии, Биологический факультет

Ленинские горы, д. 1, стр. 12, г. Москва, 119234

Radmir R. Yenikeyev — Department of Microbiology, Faculty of Biology

1–12 Leninskiye Gory, Moscow, 119234

radmir.yenikeyev@gmail.comhttps://orcid.org/0000-0003-3783-3279ЗахарчукЛ. М.ZakharchukL. M.

Захарчук Леонид Михайлович — д. б. н., доцент кафедры микробиологии, биологический факультет 

Ленинские горы, д. 1, стр. 12, г. Москва, 119234

Leonid M. Zakharchuk — D. Sc. in Biology, Associate Professor of the Department of Microbiology, Faculty of Biology

1–12 Leninskiye Gory, Moscow, 119234

Lomonosov Moscow State UniversityРоссияLomonosov Moscow State UniversityRussian Federation202421062024693-4413Copyright © Еникеев Р.Р., Захарчук Л.М., 20242024Еникеев Р.Р., Захарчук Л.М.Yenikeyev R.R., Zakharchuk L.M.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.antibiotics-chemotherapy.ru/jour/article/view/1119Актуальность

Актуальность. К настоящему времени мало данных о клинических характеристиках бактерий рода Bacillus, обитающих в чистых комнатах и асептических помещениях.

Цель исследования — идентификация и определение устойчивости к клинически значимым антибиотикам штаммов Bacillus, выделенных с Международной космической станции и медицинской лаборатории.

Материалы и методы

Материалы и методы. Идентификация изолятов осуществлена методами анализа гена 16S рРНК, MALDI-TOF и полногеномного секвенирования. Чувствительность к антибиотикам оценивали диско-диффузионным методом.

Результаты

Результаты. У семи штаммов Bacillus из 13 обнаружена резистентность к имипенему, у каждого из B. cereus LR2HG21, HSA01, HSA03, HSA12 — к имипенему, ципрофлоксацину, левофлоксацину и норфлоксацину. Полногеномное секвенирование B. cereus LR2HG21, HSA01, HSA03, HSA12 и B. safensis SE192, устойчивых к имипенему и меропенему, показало, что резистентность к ним обеспечивает ген TEM-116. Кроме TEM-116, устойчивость B. cereus LR2HG21 к имипенему и меропенему, а B. cereus HSA01 и HSA03 к имипенему обеспечивают гены BcI и/или BcII. Резистентность к эритромицину у B. subtilis SE15 и B. subtilis SE171 кодирует ген mphK.

Заключение

Заключение. У разных штаммов Bacillus устойчивость к определённому антибиотику может обеспечиваться одним или несколькими механизмами одновременно.

Background

Background. To date, there is little data on the clinical characteristics of Bacillus bacteria found in clean rooms and aseptic facilities.

The aim of the study was to identify and determine the resistance of Bacillus strains isolated from the International Space Station and medical laboratory to clinically significant antibiotics.

Methods

Methods. Isolates were identified using 16S rRNA gene analysis, MALDI-TOF, and whole-genome sequencing. Antibiotic sensitivity was assessed using the disk diffusion method.

Results

Results. Seven Bacillus strains out of 13 showed resistance to imipenem, and each of B. cereus LR2HG21, HSA01, HSA03, and HSA12 showed resistance to imipenem, ciprofloxacin, levofloxacin, and norfloxacin. Whole-genome sequencing of B. cereus LR2HG21, HSA01, HSA03, HSA12 and B. safensis SE192, resistant to imipenem and meropenem, showed that resistance to them is provided by the TEM-116 gene. In addition to TEM-116, the resistance of B. cereus LR2HG21 to imipenem and meropenem, and B. cereus HSA01 and HSA03 to imipenem, is provided by the BcI and/or BcII genes. Resistance to erythromycin in B. subtilis SE15 and B. subtilis SE171 is encoded by the mphK gene.

Conclusion

Conclusion. Resistance to a particular antibiotic in different Bacillus strains can be achieved by one or more mechanisms simultaneously.

Международная космическая станциябактерии рода Bacillusустойчивость к антибиотикамгены устойчивостиBacillus cereusBacillus subtilisBacillus safensisInternational Space Stationbacteria of the Bacillus genusantibiotic resistanceresistance genesBacillus cereusBacillus subtilisBacillus safensisИсследование осуществлялось в рамках научного проекта по выполнению государственного задания МГУ №23-1-21 (регистрационный номер ЦИТИС 121032300094-7) без использования животных и без привлечения людей в качестве испытуемых.ReferencesNikaido H. Multidrug resistance in bacteria. Annu Rev Biochem. 2009; 78: 119-146. doi: 10.1146/annurev.biochem.78.082907.145923.Nikaido H. Multidrug resistance in bacteria. Annu Rev Biochem. 2009; 78: 119-146. doi: 10.1146/annurev.biochem.78.082907.145923.Freedberg D.E., Salmasian H., Cohen B., Abrams J.A., Larson E.L. Receipt of antibiotics in hospitalized patients and risk for Clostridium difficile infection in subsequent patients who occupy the same bed. 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The authors declare that there are no conflicts of interest present.