antibioticsАнтибиотики и ХимиотерапияAntibiot Khimioter = Antibiotics and Chemotherapy0235-2990ООО «Издательство ОКИ»antibiotics-24Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL PAPERSПротивовирусная активность экстракта ладанника шалфеелистного (Cistus salviifolius) в отношении вируса гриппаThe Antiviral Activity of the Sage-Leaved Rockrose Extract (Cistus Salviifolius) Against the Influenza VirusЛаврентьеваИ. Н.LavrentievaI. N.noemail@neicon.ruСухобаевскаяЛ. П.SukhobaevskayaL. P.noemail@neicon.ruЗарубаевВ. В.ZarubayevV. V.noemail@neicon.ruНИИ эпидемиологии и микробиологии им. ПастераРоссияSaint-Petersburg Pasteur InstituteRussian Federation201706052020625-61220Copyright © ООО «Издательство ОКИ», 20202020ООО «Издательство ОКИ»ООО «Издательство ОКИ»https://www.antibiotics-chemotherapy.ru/jour/about/submissions#copyrightNoticehttps://www.antibiotics-chemotherapy.ru/jour/article/view/24

Проведено исследование протективной активности экстракта ладанника шалфеелистного (Cistus salviifolius) и биологически активной добавки Форцис в культуре клеток MDCK и на модели летальной гриппозной пневмонии у белых мышей. Изученный экстракт ингибировал репродукцию всех использованных вирусов гриппа. Наиболее чувствительными к нему оказались вирусы A/California/07/09 (H1N1)pdm09, A/mallard/Pennsylvania/10218/84 (H5N2) и A/Vladivostok/02/09 (H1N1). Для них значения IC50 составили 0,5; 0,5 и 0,4 мкг/мл, а индексы селективности - 382, 382 и 478, соответственно, что характерно для препаратов с высокой противовирусной активностью. Вирусы гриппа типа B были менее чувствительны к экстракту. Ингибирующая активность экстракта не зависела от чувствительности или устойчивости вирусов к используемым противогриппозным препаратам - осельтамивиру и ремантадину. При гриппозной пневмонии у животных снижение специфической смертности составило 40% по сравнению с группой плацебо. Применение биологически активной добавки Форцис не влияло на инфекционную активность вируса гриппа в ткани легких животных и приводило к умеренному ограничению степени поражения ткани лёгких. Максимальная эффективность биологически активной добавки «Форцис» достигалась при лечебно-профилактическом режиме её использования.

A study of the protective activity of the Sage-leaved Rockrose extract (Cistus salviifolius) and the biologically active additive Forcys in the MDCK cell culture and on the model of lethal influenza pneumonia in white mice was carried out.The studied extract inhibited the reproduction of all used influenza viruses. The most sensitive to it were the viruses A/California/07/09 (H1N1)pdm09, A/mallard/Pennsylvania/10218/84 (H5N2), and A/Vladivostok/02/09 (H1N1). The IC50 values of the listed viruses were 0.5, 0.5, and 0.4 μg/ml, and the selectivity indexes were 382, 382, and 478, respectively, which is characteristic for preparations with high antiviral activity. Type B influenza viruses were less sensitive to the extract.The inhibitory activity of the extract did not depend on the susceptibility or resistance of the viruses to the anti-influenza drugs used, oseltamivir and rimantadine. The reduction in specific mortality with influenza pneumonia in animals was 40% compared with the placebo group.The use of the biologically active supplement Forcys did not affect the infectious activity of the influenza virus in the tissues of the animals lungs and led to a moderate limitation of the degree of damage to lung tissue. The maximum effectiveness of the biologically active supplement Forcys was achieved with the therapeutic and prophylactic regimens of its use.

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The authors declare that there are no conflicts of interest present.