antibioticsАнтибиотики и ХимиотерапияAntibiot Khimioter = Antibiotics and Chemotherapy0235-2990ООО «Издательство ОКИ»antibiotics-278Research ArticleОБЗОРЫREVIEWSМеханизм действия макролидного антибиотика филипина на клеточные и липидные мембраныMechanism of Action of Macrolide Antibiotic Filipin on Cell and Lipid MembranesСамедоваА. А.SamedovaA. A.noemail@neicon.ruКасумовХ. М.KasumovKH. M.noemail@neicon.ruИнститут ботаники Национальной академии наук Азербайджана, БакуРоссияInstitute of Botany, Azerbaijan National Academy of Sciences, BakuRussian Federation2009130520205411-124452Copyright © Самедова А.А., Касумов Х.М., 20202020Самедова А.А., Касумов Х.М.Samedova A.A., Kasumov K.M.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.antibiotics-chemotherapy.ru/jour/article/view/278

В данном обзоре изложены результаты по исследованию механизма действия макролидного пентаенового антибиотика филипина на клеточные и бислойные липидные мембраны (БЛМ). Экспериментально подтверждена «стериновая гипотеза» механизма действия филипина. Изучено гемолитическое и генетико-цитологическое действие филипина, изложены данные действия филипина на вирусные инфекции и на болезни, вызываемые прионовыми белками. Представлены данные исследования механизма действия филипина на БЛМ. При малых концентрациях филипина (1 • 10-8 М) в водном солевом растворе на мембранах с холестерином обнаружены одиночные ионные каналы с проводимостью 15-20 пСм, что примерно в 3-4 раза превышает проводимость амфотерициновых каналов. Обнаружены комбинированные каналы филипина и амфотерицина В, проводимость которых составляет 25-30 пСм, что в 1,5-2 раза выше «чистых» филипиновых каналов и примерно в 5 раз выше «чистых» амфотерициновых каналов. Избирательная проницаемость филипиновых каналов преимущественно катионная.

Тhе review deals with investigation of the mechanism of action of macrolide pentaene antibiotic fllipin on cell and bilayer lipid membranes (BLM). The sterol hypothesis of the fillpin mechanism of action is confirmed experimentally. The hemolytic and genetico-cytological action of filipin, its action on virus infection and prion protein-associated diseases are discussed. The data on the mechanism of filipin action on BLM are presented. Filipin single ionic channels with conductance of 15-20 pS that is 3-4 times higher than that of the amphotericin channels were detected on cholesterol-containing membranes in filipin low concentration (1 • 10-8 M) aqueous salt solution. Combined ionic channels of filipin and amphotericin B with conductance of 25-30 pS, that is 1.5-2 time lower than that of the clean filipin channels and 5 times higher that of the clean amphotericin B channels were also detected. The selectivity of filipin channels is mainly cationic. The potential of the penetrating ion on 10-fold gradient is +18 mV. The practical aspects of filipin application in biology, medicine and pharmacology are indicated.

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The authors declare that there are no conflicts of interest present.