antibioticsАнтибиотики и ХимиотерапияAntibiot Khimioter = Antibiotics and Chemotherapy0235-2990ООО «Издательство ОКИ»antibiotics-309Research ArticleВ ПОМОЩЬ ПРАКТИКУЮЩЕМУ ВРАЧУGUIDELINES FOR PRACTITIONERSХарактеристики атипичных форм стафилококков (SCVs), выделенных от больных остеомиелитомCharacteristics of Atypical Forms of Staphylococci (SCVs) Isolated from Patients with OsteomyelitisЧуркинаЛ. Н.ChurkinaL. N.noemail@neicon.ruБидненкоС. И.BidnenkoS. I.noemail@neicon.ruМакушенкоА. С.MakushenkoA. S.noemail@neicon.ruВанечуттеМ.VaneechoutteM.noemail@neicon.ruЛюткоО. Б.LyutkoO. B.noemail@neicon.ruОзерянскаяН. М.OzeryanskayaN. M.noemail@neicon.ruАвдееваЛ. В.AvdeevaL. V.noemail@neicon.ruИнститут микробиологии и вирусологии НАН УкраиныРоссияInstitute of Microbiology and Virology, National Academy of Sciences of the UkraineRussian FederationИнститут ортопедии и травматологии АМН УкраиныРоссияInstitute of Orthopedics and Traumatology, Medical Academy of Sciences of the UkraineRussian FederationИнститут эпидемиологии и инфекционных болезней АМН УкраиныРоссияInstitute of Epidemiology and Infectious Diseases, Medical Academy of Sciences of the UkraineRussian FederationГентский университетРоссияGhent UniversityRussian Federation201013052020555-63640Copyright © Чуркина Л.Н., Бидненко С.И., Макушенко А.С., Ванечутте М., Лютко О.Б., Озерянская Н.М., Авдеева Л.В., 20202020Чуркина Л.Н., Бидненко С.И., Макушенко А.С., Ванечутте М., Лютко О.Б., Озерянская Н.М., Авдеева Л.В.Churkina L.N., Bidnenko S.I., Makushenko A.S., Vaneechoutte M., Lyutko O.B., Ozeryanskaya N.M., Avdeeva L.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.antibiotics-chemotherapy.ru/jour/article/view/309

Атипичные формы стафилококков, выделенные из популяции родительских штаммов Staphylococcus aureus, Staphylococcus haemolyticus, Staphylococcus epidermidis, теряли ряд типичный для данного рода и вида свойств и характеризовались изменённой морфологией колоний, замедленным ростом, отсутствием пигментации, а также изменённой формой утилизации углеводов. Для SCVs S.aureus характерно отсутствие плазмокоагулазной и лецитиназной активностей. Дальнейший анализ 14 вариантов SCVs показал, что они являются ауксотрофами по гемину и менадиону и обладают устойчивостью к аминогликозидным антибиотикам. Эти фенотипические характеристики микроорганизмов усложняют и во многих случаях делают невозможной их идентификацию общепринятыми в клинических лабораториях методами. Методы генотипической идентификации, в частности tRNA-PCR анализ, позволяют более достоверно идентифицировать атипичные формы стафилококков с таким сложным фенотипом.

Small-colony variants (SCVs), Isolated from a population of the parental strains of Staphylococcus aureus, S.haemolyticus and S.epidermidis lost a number of features typical of the species and genus and were characterized by delayed growth, altered colony morphology, lack of pigmentation and changed carbohydrate consumption. Some SCVs of S.aureus had no plasmocoagulase and lecithinase activities. The analysis of 14 SCVs showed that they were auxotrophic for hemin and menadione and resistant to aminoglycoside antibiotics. Such aberrant phenotypic characteristics complicated or made it impossible their identification by the common clinical laboratory methods. The tRNA intergenic spacer length polymorphism analysis was used to identify the atypical forms of the staphylococci.

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The authors declare that there are no conflicts of interest present.