antibioticsАнтибиотики и ХимиотерапияAntibiot Khimioter = Antibiotics and Chemotherapy0235-2990ООО «Издательство ОКИ»antibiotics-338Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL PAPERSГенетическое окружение генов blaCTX_M, локализованных на конъюгативных плазмидах нозокомиальных изолятов Enterobacteriaceae, выделенных в России в 2003-2007 ггGenetic Environments of blaCTX-M Genes Located on Conjugative Plasmids of Enterobacteriaceae Nosocomial Isolates Collected in Russia within 2003-2007ФурсоваН. К.FursovaN. K.noemail@neicon.ruПрямчукС. Д.PryamchukS. D.noemail@neicon.ruАбаевИ. В.AbaevI. V.noemail@neicon.ruКовалевЮ. Н.KovalevYU. N.noemail@neicon.ruШишковаН. А.ShishkovaN. A.noemail@neicon.ruПечерскихЭ. И.PecherskikhE. I.noemail@neicon.ruКоробоваО. В.KorobovaO. V.noemail@neicon.ruАсташкинЕ. И.AstashkinE. I.noemail@neicon.ruПачкуновД. М.PachkunovD. M.noemail@neicon.ruСветочЭ. А.SvetochE. A.noemail@neicon.ruСидоренкоС. В.SidorenkoS. V.noemail@neicon.ruГосударственный научный центр Прикладной микробиологии и биотехнологии Роспотребнадзора, ОболенскРоссияState Research Centre for Applied Microbiology and Biotechnology, ObolenskRussian FederationНаучно-исследовательский институт детских инфекций ФМБА, Санкт-ПетербургРоссияResearch Institute for Children' Infections, St.PetersburgRussian Federation2010130520205511-12310Copyright © Фурсова Н.К., Прямчук С.Д., Абаев И.В., Ковалев Ю.Н., Шишкова Н.А., Печерских Э.И., Коробова О.В., Асташкин Е.И., Пачкунов Д.М., Светоч Э.А., Сидоренко С.В., 20202020Фурсова Н.К., Прямчук С.Д., Абаев И.В., Ковалев Ю.Н., Шишкова Н.А., Печерских Э.И., Коробова О.В., Асташкин Е.И., Пачкунов Д.М., Светоч Э.А., Сидоренко С.В.Fursova N.K., Pryamchuk S.D., Abaev I.V., Kovalev Y.N., Shishkova N.A., Pecherskikh E.I., Korobova O.V., Astashkin E.I., Pachkunov D.M., Svetoch E.A., Sidorenko S.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.antibiotics-chemotherapy.ru/jour/article/view/338

Показано, что гены blaCTX-M присутствуют в геномах 71% устойчивых к цефалоспоринам нозокомиальных изолятов семейства Enterobacteriaceae (и=833), выделенных в России в 2003-2007 гг., в том числе в 91% культур E.coli, 90% - Klebsiella spp., 38% - Enterobacter spp., 31% - Citrobacter spp. (n=9) и 36% культур других видов энтеробактерий. Идентифицированы гены подтипов (кластеров): blaCTX-M-1 (529 генов blaCTX-M-15; 25 генов blaCTX-M-3; по одному гену blaCTX-M-22, blaCTX-M-23 и blaCTX-M-34; blaCTX-M-2 (один ген blaCTX-M-2 и четыре гена blaCTX-M-5 и blaCTX-M-9 (два гена blaCTX-M-9 и 28 генов blaCTX-M-14). Гены blaCTX-M локализованы на высокомолекулярных (60-160 т.п.н.) коньюгативных плазмидах, принадлежащих преимущественно к группам несовместимости IncF, IncL/M и IncA/C (blaCTX-M-15); IncL/M (blaCTX-M-3); IncF, IncL/M и IncIl-ly (blaCTX-M-14). Генетическое окружение специфично для разных генов blaCTX-M: перед генами blaCTX-M-3, blaCTX-M-14 и blaCTX-M-15 локализована последовательность ISEcp1, демонстрирующая большое разнообразие перестроек (делеции разной протяжённости, а также инсерции последовательностей IS26, IS1, IS10, resTn2 и resTn3 в разных ориентациях). Характерно расстояние между ISEcp1 и геном blaCTX-M: 48 п.н. - для гена blaCTX-M-15 (кроме одного изолята E.coli, у которого данная последовательность укорочена на 3 п.н.); 127 п.н. - для гена blaCTX_M_3; 42 п.н. - для гена blaCTX-M-14. После гена blaCTX_M_3 локализована последовательность orf477-mucA; после гена blaCTX_M_15 - последовательность Aorf477-AmucA (в двух изолятах имеющая вставку Aorf3); а после гена blaCTX-M-14 - последовательность IS903 (цельная или укороченная). Гены blaCTX-M-2 и blaCTX-M-9 локализованы в структуре ISCR1, после интегрона класса 1 и последовательности orf513.

The study showed that blaCTX-M genes were present in the genomes of 71% of сephаlosporin resistant Enterobacteriaceae nosocomial isolates (n=833) collected in Russian hospitals within 2003-2007, including 91% of E.coli, 90% of Klebsiella spp., 38% of Enterobacter spp., 31 % of Citrobacter spp. (n=9), and 36% of the other Enterobacteriaceae species. The genes belonging to the following subtypes (clusters) were identified: blaCΎX_M_1 (529 blaCTX_M_15 genes; 25 blaCTX_M_3 genes; 1 blaCTX_M_22 gene, 1 blaCTX-M-23 gene and 1 blaCTX-M-34 gene); blaCTX-M-2 (1 blaCTX-M-2 gene and 4 blaCTX-M-5 genes), and blaCTX-M-9 (2 blaCTX-M_9 genes, and 28 blaCTX_M_14 genes). It was shown that blaCTX-M genes were located on high-molecular weight (60-160 bp) conjugative plasmids belonging mainly to the incompatibility groups IncF, IncL/M and IncA/C (blaCTX-M-15 gene); IncL/M (blaCTX-M-3 gene); and IncF, IncL/M and IncIl-ly (CTX-M-14 gene). The gene environments of blaCTX-M genes were shown specific for the subtype of the genes. A mobile genetic element lSEcp1 (in some cases deleted or inserted by IS26, IS1, IS10, resTn2, or resTn3 sequences, in direct or reverse position) were detected upstream of blaCTX-M-3, blaCTX-M-14, and blaCTX-M-15 genes. A special characteristic was the sequence between ISEcpl and blaCTX-M gene: 48 bp for blaCTX-M-15 (except 1 E.coli isolate having such a sequence deleted by 3 bp); 127 bp for blaCTX-M-3; 42 bp for blaCTX-M-14. Downstream of blaCTX-M and blaCTX-M-15 genes in the major bacterial isolates orf477 mucA and Aorf477-AmucA sequences were detected respectively. Two isolates had additional Aorf3 insertion inside of Aorf477-AmucA sequence. Insertion sequence IS903 (intact or deleted) was detected downstream of blaCTX-M-14 gene. Unlike the others, blaCTX-M-2 and blaCTX-M-9 genes were located inside of IS CR1 mobile element, downstream of class 1 integron and orf513 sequence.

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The authors declare that there are no conflicts of interest present.