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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">antibiotics</journal-id><journal-title-group><journal-title xml:lang="ru">Антибиотики и Химиотерапия</journal-title><trans-title-group xml:lang="en"><trans-title>Antibiot Khimioter = Antibiotics and Chemotherapy</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">0235-2990</issn><publisher><publisher-name>ООО «Издательство ОКИ»</publisher-name></publisher></journal-meta><article-meta><article-id custom-type="elpub" pub-id-type="custom">antibiotics-443</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>ОРИГИНАЛЬНЫЕ СТАТЬИ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>ORIGINAL PAPERS</subject></subj-group></article-categories><title-group><article-title>Погружённое культивирование и химический состав мицелия Hericium erinaceus</article-title><trans-title-group xml:lang="en"><trans-title>Submerged Cultivation and Chemical Composition of Hericium erinaceus Mycelium</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Автономова</surname><given-names>А. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Avtonomova</surname><given-names>A. V.</given-names></name></name-alternatives><email xlink:type="simple">noemail@neicon.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Баканов</surname><given-names>А. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Bakanov</surname><given-names>A. V.</given-names></name></name-alternatives><email xlink:type="simple">noemail@neicon.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Шуктуева</surname><given-names>М. И.</given-names></name><name name-style="western" xml:lang="en"><surname>Shuktueva</surname><given-names>M. I.</given-names></name></name-alternatives><email xlink:type="simple">noemail@neicon.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Винокуров</surname><given-names>В. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Vinokurov</surname><given-names>V. A.</given-names></name></name-alternatives><email xlink:type="simple">noemail@neicon.ru</email><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Попова</surname><given-names>О. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Popova</surname><given-names>O. V.</given-names></name></name-alternatives><email xlink:type="simple">noemail@neicon.ru</email><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Усов</surname><given-names>А. И.</given-names></name><name name-style="western" xml:lang="en"><surname>Usov</surname><given-names>A. I.</given-names></name></name-alternatives><email xlink:type="simple">noemail@neicon.ru</email><xref ref-type="aff" rid="aff-3"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Краснопольская</surname><given-names>Л. М.</given-names></name><name name-style="western" xml:lang="en"><surname>Krasnopolskaya</surname><given-names>L. M.</given-names></name></name-alternatives><email xlink:type="simple">noemail@neicon.ru</email><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff xml:lang="ru" id="aff-1"><institution>НИИ по изысканию новых антибиотиков им .Г.Ф. Гаузе РАМН</institution><country>Russian Federation</country></aff><aff xml:lang="ru" id="aff-2"><institution>Российский государственный университет нефти и газа им. И.М. Губкина</institution><country>Russian Federation</country></aff><aff xml:lang="ru" id="aff-3"><institution>Институт органической химии им. Н.Д. Зелинского РАН</institution><country>Russian Federation</country></aff><pub-date pub-type="collection"><year>2012</year></pub-date><pub-date pub-type="epub"><day>13</day><month>05</month><year>2020</year></pub-date><volume>57</volume><issue>7-8</issue><fpage>7</fpage><lpage>11</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; ООО «Издательство ОКИ», 2020</copyright-statement><copyright-year>2020</copyright-year><copyright-holder xml:lang="ru">ООО «Издательство ОКИ»</copyright-holder><copyright-holder xml:lang="en">ООО «Издательство ОКИ»</copyright-holder><license xlink:href="https://www.antibiotics-chemotherapy.ru/jour/about/submissions#copyrightNotice" xlink:type="simple"><license-p>https://www.antibiotics-chemotherapy.ru/jour/about/submissions#copyrightNotice</license-p></license></permissions><self-uri xlink:href="https://www.antibiotics-chemotherapy.ru/jour/article/view/443">https://www.antibiotics-chemotherapy.ru/jour/article/view/443</self-uri><abstract><p>На основе изучения трофических потребностей лекарственного гриба Hericium еrinaceus и оптимизации одной из наиболее перспективных жидких питательных сред разработан способ погружённого культивирования, обеспечивающий получение 22-23 г/л воздушно-сухой биомассы на 7-е сутки ферментации. Способ погружённого культивирования масштабирован для условий лабораторного ферментёра. Изучен химический состав мицелия H.erinaceus, полученного по разработанному способу. По содержанию всех незаменимых аминокислот за исключением триптофана биомасса Н.erinaceus соответствовала требованиям ФАО/ВОЗ. Основными жирными кислотами мицелия H.erinaceus являются олеиновая и линолевая. Содержание водорастворимых полисахаридов составляет 19%. Выделены две фракции водорастворимых полисахаридов, содержащие в своём составе рамнозу, фукозу, ксилозу, глюкозу и галактозу. В погружённой биомассе H.erinaceus обнаружены витамины В1, В2, В6, PP и Е, эргостерин, коэнзим Q.</p></abstract><trans-abstract xml:lang="en"><p>Submerged cultivation of Hericium erinaceus in various media was studied. The yield of the biomass was shown to depend mainly on the carbon source, whereas the content of water soluble polysaccharides depended mainly on the nitrogen source. The optimal medium composition provided the biomass yield of 21-23 g/l in 7 days. The biomass was characterized by the content of total protein, lipids and carbohydrates. In addition, the amino acid composition of the biomass was determined and shown to meet all the requirements of FAO/WHO concerning the amounts of essential amino acids (with exception of tryptophane). Oleinic and linoleic acids were identified as the main components of the fatty acids. Two water soluble polysaccharide fractions differing in solubility in aqueous ethanol were isolated and shown to contain rhamnose, fucose, xylose, glucose and galactose in different proportions. Vitamins B1, B2, B6, PP and E, ergosterol and coenzyme Q were also detected in the biomass of H.erinaceus.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>погружённое культивирование</kwd><kwd>мицелий Hericium erinaceus</kwd></kwd-group><kwd-group xml:lang="en"><kwd>submerged cultivation</kwd><kwd>Hericium erinaceus</kwd></kwd-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Wang J.C.Б Hu S.H., Lee T.M. Antitumor and immunoenhancing activities of polysaccharide from culture broth of Hericium spp. Kaoshing J Med Sci 2001; 17: 9: 461-467.</mixed-citation><mixed-citation xml:lang="en">Wang J.C.Б Hu S.H., Lee T.M. 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