antibioticsАнтибиотики и ХимиотерапияAntibiot Khimioter = Antibiotics and Chemotherapy0235-2990ООО «Издательство ОКИ»antibiotics-673Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL PAPERSВлияние сульфатированныгх полисахаридов из бурой водоросли Fucus evanescens и продукта их ферментативной трансформации на функциональную активность клеток врожденного иммунитетаEffect of Sulfated Polysaccharides from Brown Alga Fucus evanescens and Their Enzymatic Transformation Product on Functional Activity of Innate Immunity CellsКузнецоваТ. А.KuznetsovaT. A.noemail@neicon.ruСмолинаТ. П.SmolinaT. P.noemail@neicon.ruБеседноваН. Н.BesednovaN. N.noemail@neicon.ruСильченкоА. С.SilchenkoA. S.noemail@neicon.ruИмбсТ. И.ImbsT. I.noemail@neicon.ruЕрмаковаС. П.ErmakovaS. P.noemail@neicon.ruНИИ эпидемиологии и микробиологии им. Г. П. СомоваРоссияG.P. Somov Research Institute of Epidemiology and MicrobiologyRussian FederationТихоокеанский институт биоорганической химии им. Г. Б. Елякова ДВО РАНРоссияG.B. Elyakov Pacific Institute of Bioorganic ChemistryRussian Federation201613052020617-81014Copyright © ООО «Издательство ОКИ», 20202020ООО «Издательство ОКИ»ООО «Издательство ОКИ»https://www.antibiotics-chemotherapy.ru/jour/about/submissions#copyrightNoticehttps://www.antibiotics-chemotherapy.ru/jour/article/view/673

Проведено сравнительное изучение влияния сульфатированных полисахаридов (ПС) из бурой водоросли Fucus evanescens и низкомолекулярного продукта ферментативной трансформации на функциональную активность клеток врождённого иммунитета - полиморфноядерных лейкоцитов периферической крови человека (NF). В результате контакта NF in vitro с образцами ПС зарегистрированы значимые изменения функционального состояния клеток. Это выражалось в усилении плотности молекул CD69, CD14, CD11b на клеточных мембранах с одновременным снижением CD62L и в увеличении показателей фагоцитарной и бактерицидной активности NF. Низкомолекулярный образец, полученный из фукоидана с применением фукоиданаз, оказывает более выраженный эффект на уровень экспрессии молекул CD14, CD11b и CD62L по сравнению с высокомолекулярным.

The effect of sulfated polysaccharides (PS) from brown alga Fucus evanescens and their enzymatic transformation and low molecular weight product on the functional activity of the innate immunity cells, i.e. polymorphonuclear leukocytes of human peripheral blood (NF) was comparatively studied. The in vitro NF contact with PS resulted in significant changes in the functional activity of NF, evident from higher density of molecules CD69, CD14, CD11b on the cell membranes with simultaneous lowering of that of CD62L and increased phagocytic and bactericidal activity of NF. The low molecular weight product resulting from fucoidan transformation with fucoidanases showed a higher effect on the level of the molecules CD14, CD11b and CD62L expression vs. the high molecular weight PS.

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The authors declare that there are no conflicts of interest present.