antibioticsАнтибиотики и ХимиотерапияAntibiot Khimioter = Antibiotics and Chemotherapy0235-2990ООО «Издательство ОКИ»antibiotics-694Research ArticleВ ПОМОЩЬ ПРАКТИКУЮЩЕМУ ВРАЧУGUIDELINES FOR PRACTITIONERSSNP-полиморфизм в геномах изолятов Streptococcus pneumoniae CC320, устойчивых к бета-лактамным антибиотикамSNP Polymorphism in Genomes of CC320 Isolates of Streptococcus pneumoniae Resistant to Beta-LactamsЦветковаИ. А.TzvetkovaI. A.noemail@neicon.ruВолковаМ. О.VolkovaM. Q.noemail@neicon.ruКалиногорскаяО. С.KalinogorskayaQ. S.noemail@neicon.ruБелановС. С.BelanovS. S.noemail@neicon.ruГостевВ. В.GostevV. V.noemail@neicon.ruСидоренкоС. В.SidorenkoS. V.noemail@neicon.ruНИИ детских инфекций ФМБА РоссииRussian FederationУниверситет Хельсинки, Институт БиотехнологииRussian FederationНИИ детских инфекций ФМБА России; Северо-Западный Государственный Медицинский Университет им. И.И. МечниковаRussian Federation2016130520206111-122127Copyright © Цветкова И.А., Волкова М.О., Калиногорская О.С., Беланов С.С., Гостев В.В., Сидоренко С.В., 20202020Цветкова И.А., Волкова М.О., Калиногорская О.С., Беланов С.С., Гостев В.В., Сидоренко С.В.Tzvetkova I.A., Volkova M.Q., Kalinogorskaya Q.S., Belanov S.S., Gostev V.V., Sidorenko S.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.antibiotics-chemotherapy.ru/jour/article/view/694

В результате биоинформационного анализа данных полногеномного секвенирования изолятов Streptococcus pneumoniae клонального комплекса СС320, циркулирующих в Российской Федерации, а также данных об изолятах СС320 из открытых источников у резистентных к пенициллину изолятов было обнаружено 139 миссенс-мутаций в 45 генах. Кроме мутаций в генах основных пенициллин-связывающих белков (ПСБ - PBP1A, PBP2B и PBP2X) высокая частота мутаций обнаружена в генах, входящих в состав dcw-клагтера (division and cell wall), а также в белке RegR, который относится к регуляторам транскрипции, принадлежащим к семейству LacI/GalR. Формирование резистентности к бета-лактамным антибиотикам у S.pneumoniae определяется не только модификацией ПСБ, но и адаптационными изменениями в метаболических путях участвующих в росте делении бактериальной клетки.

Bioinformatic analysis of the data on the genome sequencing of the isolates of the Streptococcus pneumoniae clonal complex SS320 from the Russian Federation, as well as the data on SS320 isolates from public sources in the penicillin resistant isolates resulted in detection of 139 missense mutations in 45 genes. In addition to the mutations in the genes of the main penicillin-binding proteins (PSB - PBP1A, PBP2B and PBP2X) there was detected high frequency of mutations in the genes of the (division and cell wall) dcw-cluster, as well as in RegR protein belonging to the transcription regulators of the LacI/GalR family. Development of resistance to beta-lactams in S.pneumoniae is defined not only by modification of the PSB, but also by adaptive changes in the metabolic pathways involved in the bacterial cell growth and division.

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The authors declare that there are no conflicts of interest present.