antibioticsАнтибиотики и ХимиотерапияAntibiot Khimioter = Antibiotics and Chemotherapy0235-2990ООО «Издательство ОКИ»antibiotics-70Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL PAPERSИсследование in vitro противоопухолевой и антимикробной активности препарата пэгилированных липосом с СангвинариномIn Vitro Study of Antitumor and Antimicrobial Activity of a Preparation of Pegylated Liposomes with SanguinarineЛуценкоС. В.LutsenkoS. V.noemail@neicon.ruГромовыхТ. И.GromovykhT. I.noemail@neicon.ruКаширинВ. В.KashirinV. V.noemail@neicon.ruКурьяковВ. Н.KuryakovV. N.noemail@neicon.ruБарановаА. А.BaranovaA. A.noemail@neicon.ruСадыковаВ. С.SadykovaV. S.noemail@neicon.ruФельдманН. Б.FeldmanN. B.noemail@neicon.ruПервый московский государственный медицинский университет им. И. М. Сеченова Минздрава РоссииРоссияI. M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian FederationRussian FederationИнститут проблем нефти и газа РАНРоссияOil and Gas Research Institute RASRussian FederationНИИ по изысканию новых антибиотиков имени Г.Ф. ГаузеРоссияGause Institute of New AntibioticsRussian Federation201806052020633-437Copyright © Луценко С.В., Громовых Т.И., Каширин В.В., Курьяков В.Н., Баранова А.А., Садыкова В.С., Фельдман Н.Б., 20202020Луценко С.В., Громовых Т.И., Каширин В.В., Курьяков В.Н., Баранова А.А., Садыкова В.С., Фельдман Н.Б.Lutsenko S.V., Gromovykh T.I., Kashirin V.V., Kuryakov V.N., Baranova A.A., Sadykova V.S., Feldman N.B.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.antibiotics-chemotherapy.ru/jour/article/view/70

Сангвинарин представляет собой растительный алкалоид, обладающий широким спектром биологической активности. Эффективность действия сангвинарина может быггь увеличена путём его интегрирования в липосомные наночастицы. Целью настоящего исследования являлось получение и очистка пэгилированного липосомного сангвинарина, изучение его свойств, а также противоопухолевой и антимикробной активности in vitro. Были получены пэгилированные липосомные наночастицы, содержащие сангвинарин. Размер липосом составлял 61,8+5,7 нм; эффективность включения сангвинарина в липосомы составляла 82,3+4,9%. Изучено высвобождение сангвинарина из липосомных частиц; продемонстрирован его пролонгированный характер. Исследование in vitro показало, что липосомный сангвинарин проявлял дозозависимую цитотоксическую активность в отношении опухолевый клеток линий MCF-7 (12,8 мкМ), L1210 (17,4 мкМ ), А431 (18,67 мкМ) и HepG2 (20,7 мкМ). Антимикробное действие липосомной формы препарата было установлено в отношении грамположительных (B.subtilis АТСС 6633 и B.coagulans 429) и грамотрицательных (E.coli ATCC 8739) бактерий, а также условно-патогеннык грибов Aspergillus ustus 6К. Полученные результаты свидетельствуют о перспективности дальнейшего исследования пэгилированного липосомного сангвинарина в качестве противоопухолевого и антимикробного агента.

Sanguinarin is a plant alkaloid with a wide spectrum of biological activity. The effectiveness of sanguinarin can be increased by its integration into liposomal nanoparticles. The aim of this study was to obtain and purify the pegylated liposome sanguinarine, to study its properties, as well as antitumor and antimicrobial activity in vitro. Pegylated liposome nanoparticles containing sanguinarinewere obtained. The size of the liposomes was 61.8+5.7 nm; the effectiveness of the inclusion of sanguinarine in liposomes was 82.3+4.9%. The article studies the release of sanguinarin from liposome particles and demonstrates its prolonged nature. An in vitro study showed that liposomal sanguinarine exhibited dose-dependent cytotoxic activity against tumor cells of MCF-7 (12.8 μM), L1210 (17.4 μM), A431 (18.67 μM) and HepG2 (20.7 μM). The antimicrobial effect of the liposome form of the drug was established for Gram-positive (B.subtilis ATC 6633 and B.coagulans 429) and Gram-negative (E.coli ATCC 8739) bacteria, as well as opportunistic fungi Aspergillus ustus 6K. The results indicate the prospects of further study of pegylated liposomal sanguinarine as an antitumor and antimicrobial agent.

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The authors declare that there are no conflicts of interest present.