antibioticsАнтибиотики и ХимиотерапияAntibiot Khimioter = Antibiotics and Chemotherapy0235-2990ООО «Издательство ОКИ»10.37489/0235-2990-2020-65-5-6-3-10antibiotics-733Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL PAPERSРоль сульфатных групп в фукоидане из Fucus evanescens в стимуляции продукции провоспалительных цитокинов клетками периферической крови человека in vitroThe Role of Sulfates in Fucoidan Extracted from Fucus evanescens in Proinflammatory Cytokines Production by Human Peripheral Blood Cells in vitroХильченкоС. Р.Khil'chenkoS. R.

Хильченко Станислав Русланович — научный сотрудник

23562, Любек, Ратцебургер алли, 160

Lübeck

stanislav.khilchenko@uksh.deЗапорожецТ. С.ZaporozhetsT. S.

Запорожец Татьяна Станиславовна — д. м. н., заместитель директора по научной работе

Владивосток

Vladivostok

ЗвягинцеваТ. Н.ZvyagintsevaT. N.

Звягинцева Татьяна Николаевна — д. х. н., профессор

Владивосток

Vladivostok

ШевченкоН. М.ShevchenkoN. M.

Шевченко Наталья Михайловна — к. х. н., старший научный сотрудник

Владивосток

Vladivostok

БеседноваН. Н.BesednovN. N.

Беседнова Наталья Николаевна — д. м. н., академик РАН, главный научный сотрудник

Владивосток

Vladivostok

Любекский институт экспериментальной дерматологииГерманияLübeck Institute of Experimental DermatologyGermanyНаучно-исследовательский институт эпидемиологии и микробиологии имени Г. П. СомоваРоссияSomov Institute of Epidemiology and MicrobiologyRussian FederationТихоокеанский институт биоорганической химии им. Г. Б. Елякова Дальневосточного отделения Российской академии наукРоссияG. B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch, Russian Academy of SciencesRussian Federation202026082020655-6310Copyright © Хильченко С.Р., Запорожец Т.С., Звягинцева Т.Н., Шевченко Н.М., Беседнова Н.Н., 20202020Хильченко С.Р., Запорожец Т.С., Звягинцева Т.Н., Шевченко Н.М., Беседнова Н.Н.Khil'chenko S.R., Zaporozhets T.S., Zvyagintseva T.N., Shevchenko N.M., Besednov N.N.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.antibiotics-chemotherapy.ru/jour/article/view/733

Фукоиданы, сульфатированные полисахариды бурых водорослей (Phaeophyceae), обладают широким спектром биологической активности. Молекулярная структура фукоиданов и оценка роли структурных элементов в проявлении их биологических свойств до настоящего времени остаются предметом активного изучения и уточнения. В работе представлены результаты изучения роли сульфатных и ацетильных групп в фукоидане из бурой водоросли Fucus evanescens в модуляции продукции провоспалительных цитокинов клетками гепаринизированной нефракционированной периферической крови человека (КПКЧ). Ьатериал и методы. КПКЧ инкубировали с нативным фукоиданом и его дезацетилированными и частично десульфатированными производными (100 мкг/мл). Концентрацию цитокинов в супернатантах определяли методом твёрдофазного иммуноферментного анализа с использованием коммерческих тест-систем. Результаты. Инкубирование КПКЧ с нативным фукоиданом приводило к повышению концентрации IL-6, TNF-α, IL-8 в супернатантах. Частичное удаление сульфатных групп у нативного фукоидана отменяло, либо уменьшало стимулирующий эффект в отношении продукции цитокинов IL-6, TNF-α, но не хемокина IL-8. Действие дезацетилированного фукоидана было сопоставимо с действием нативного полисахарида. Нативный полисахарид и его химически модифицированные производные не оказывали влияния на продукцию IFN-γ и IL-10. Заключение. Полученные результаты свидетельствуют о значении сульфатных групп в реализации цитокин-индуцирующих свойств фукоидана из бурой водоросли F.evanescens.

Fucoidans, sulfated polysaccharides extracted from brown algae (Phaeophyceae), have a wide spectrum of bioactivity. Studies of molecular structures of fucoidans and deciphering of molecular elements' impact on their biological activities are at their active stage. The article shows the role of sulfates and acetyl groups in fucoidan isolated from Fucus evanescens in proinflammatory cytokines production by human heparinized unfractionated peripheral blood cells. Material and Methods. The cells were incubated with native fucoidan (N) and its deacetylated (deA), partially desulfated (deS), and both deacetylated and partially desulfated (deAdeS) derivatives (100 μg/mL). Cytokine concentrations were determined in cell supernatants by ELISA in a 'sandwich' modification with commercial kits. Results. Incubation with N fucoidan led to an increase of IL-6, TNF-α, IL-8 levels in supernatants. Partial removal of sulfate groups cancelled or decreased stimulating effect for IL-6, TNF-α, cytokines, but not for IL-8. deAc fucoidan action was comparable with N polysaccharide. Native polysaccharide and its chemically modified derivatives did not change IFN-γ и IL-10 cytokine production. Conclusion. The obtained results suggest that sulfates have a significant role in cytokine-producing properties of fucoidan extracted from brown algae F.evanescens.

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The authors declare that there are no conflicts of interest present.