antibioticsАнтибиотики и ХимиотерапияAntibiot Khimioter = Antibiotics and Chemotherapy0235-2990ООО «Издательство ОКИ»10.37489/0235-2990-2021-66-9-10-12-16antibiotics-854Research ArticleЭКСПЕРИМЕНТАЛЬНЫЕ СТАТЬИExperimental ResearchАнтимикробная резистентность энтерококковAntimicrobial Resistance in Enterococcihttps://orcid.org/0000-0002-1000-2240БаранцевичН. Е.BarantsevichN. E.

Баранцевич Наталья Евгеньевна — научный сотрудник

eLIBRARY: SPIN-код: 3914-4499; Author ID: 785835. Scopus Author ID: 55880381000

Санкт-Петербург

Natalia E. Barantsevich

eLIBRARY: SPIN: 3914-4499; Author ID: 785835. Scopus Author ID: 55880381000

Saint-Petersburg

ВолковаС. В.VolkovaS. V.

Волкова Светлана Владимировна — заведующая клинической микробиологической лабораторией

Санкт-Петербург

Svetlana V. Volkova

Saint-Petersburg

ЗарицкийА. Ю.ZaritskyA. Yu.

Зарицкий Андрей Юрьевич — д. м. н., профессор, директор Института онкологии и гематологии

eLIBRARY Author ID: 109683. Scopus Author ID: 6506410026. WOS Research ID: A-8478-2016

Санкт-Петербург

Andrey Yu. Zaritskey

eLIBRARY Author ID: 109683. Scopus Author ID: 6506410026. WOS Research ID: A-8478-2016

Saint-Petersburg

https://orcid.org/0000-0002-4800-3345БаранцевичЕ. П.BarantsevichE. P.

Баранцевич Елена Петровна — д. м. н., заведующая научно-исследовательским отделом микробиологии и внутрибольничных инфекций

eLIBRARY SPIN-код: 3534-1010. AuthorID: 268934. Scopus Author ID: 6601955793. WOS Research ID: S-1455-2016

Санкт-Петербург

Elena P. Barantsevich — D.Sc. in medicine

eLIBRARY SPIN: 3534-1010. AuthorID: 268934. Scopus Author ID: 6601955793. WOS Research ID: S-1455-2016

Saint-Petersburg

lenabara2003@mail.ruФГБУ «Национальный медицинский исследовательский центр им. В. А. Алмазова» Министерства здравоохранения Российской ФедерацииРоссияAlmazov National Medical Research Centre of the Ministry of Health of the Russian FederationRussian Federation202102012022669-101216Copyright © Баранцевич Н.Е., Волкова С.В., Зарицкий А.Ю., Баранцевич Е.П., 20222022Баранцевич Н.Е., Волкова С.В., Зарицкий А.Ю., Баранцевич Е.П.Barantsevich N.E., Volkova S.V., Zaritsky A.Y., Barantsevich E.P.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.antibiotics-chemotherapy.ru/jour/article/view/854Актуальность

Актуальность. Enterococcus spp. — условно-патогенные возбудители внебольничных и госпитальных инфекций, которые в последние годы расценивают как угрозу общественному здоровью в связи с их антимикробной резистентностью, в первую очередь к гликопептидам.

Цель

Цель. Определение распространенности различных видов энтерококков — возбудителей инфекций у госпитализированных пациентов и их резистентности к антибактериальным препаратам.

Методы исследования включали в себя определение видовой принадлежности энтерококков методом MALDI-TOF масс-спектрометрии и определение их чувствительности к антибактериальным препаратам в соответствии с рекомендациями EUCAST или, при их отсутствии, CLSI.

Результаты

Результаты. Определили видовой состав и антимикробную резистентность 1562 последовательных штаммов, выделенных у госпитализированных пациентов в крупном медицинском центре, аккумулирующем пациентов из различных регионов Российской Федерации в 2019 г. Выявили преобладание видов E.faecalis и E.faecium (99,5%), частота выделения первого на 56% превышала частоту выделения последнего. Е.аvium, Е.casseliflavus, E.gallinarum, E.durans изолировали из 0,5% биологических проб. Наибольший уровень резистентности энтерококков наблюдали к эритромицину (84,8%), тетрациклину (75,0%) и рифампицину (68,2%). Мультирезистентность и резистентность к ванкомицинy были характерны преимущественно для E.faecium. Все выделенные из крови изоляты E.faecium были мультирезистентны. Устойчивость к ванкомицину у энтерококков, обусловивших развитие инфекций кровотока, наблюдали исключительно у 19,5% E.faecium, все ванкомицинорезистентные изоляты были устойчивы к тейкопланину. Резистентность к линезолиду выявили у 2 внебольничных штаммов E.faecalis (0,1%). Редкие виды энтерококков демонстрировали разнообразные паттерны антимикробной резистентности.

Заключение

Заключение. Среди энтерококков — возбудителей инфекций у госпитализированных пациентов превалируют виды E.faecalis и E.faecium. Мультирезистентность и устойчивость к ванкомицину характерны преимущественно для E.faecium, особенно для штаммов — возбудителей инфекций кровотока. Необходимо проведение дальнейшего мониторинга распространения различных видов энтерококков в госпитальных и внебольничных условиях с определением их видовой принадлежности и антимикробной резистентности валидными методами.

Background

Background. Enterococcus spp. are opportunistic agents of community-acquired and in-hospital infections, which have been considered a threat to public health due to their antimicrobial resistance, primarily to glycopeptides, in recent years.

The aim of the study is to determine the prevalence of various Enterococcus species causing infections in hospitalized patients and their antimicrobial resistance.

Methods included identification by MALDI-TOF mass spectrometry and antimicrobial susceptibility testing in accordance with the EUCAST or, in their absence, CLSI guidelines.

Results

Results. Antimicrobial resistance in 1562 consecutive Enterococcus strains isolated from hospitalized patients was determined in a major medical center admitting patients from various regions of the Russian Federation in 2019. The predominance of E.faecalis and E.faecium (99.5%) was revealed; the frequency of isolation of the former was 56% higher than that of the latter. E.avium, E.casseliflavus, E.gallinarum, E.durans were isolated from 0.5% of biological samples. The highest level of resistance of enterococci was observed to erythromycin (84.8%), tetracycline (75.0%), and rifampicin (68.2%). Multidrug, as well as vancomycin resistance, prevailed in E.faecium. All E.faecium strains isolated from blood were multidrug resistant. Resistance to vancomycin in enterococci, causing bloodstream infections, was observed solely in 19.5% of E.faecium; all vancomycin-resistant isolates were also resistant to teicoplanin. Linezolid resistance was detected in 2 community-acquired strains of E.faecalis (0.1%). Rare enterococci have shown diverse patterns of antimicrobial resistance.

Conclusions

Conclusions. E.faecalis and E.faecium prevailed among Enterococcus spp. causing infections in hospitalized patients. Multidrug resistance and vancomycin resistance were observed predominantly in E.faecium, especially in strains causing blood-stream infections. Further monitoring of the spread and antimicrobial resistance of various Enterococcus spp. in hospital and community-acquired infections is needed.

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The authors declare that there are no conflicts of interest present.