antibioticsАнтибиотики и ХимиотерапияAntibiot Khimioter = Antibiotics and Chemotherapy0235-2990ООО «Издательство ОКИ»10.37489/0235-2990-2021-66-11-12-18-24antibiotics-875Research ArticleЭКСПЕРИМЕНТАЛЬНЫЕ СТАТЬИExperimental ResearchСеротиповый состав Streptococcus pneumoniae, циркулирующих у детей с респираторными инфекциями, оптимизация молекулярных методов оценкиSerotype composition of Streptococcus pneumoniae in children with respiratory infections, optimization of molecular assessment methodshttps://orcid.org/0000-0002-9737-9496НикитинаЕ. В.NikitinaE. V.

 Никитина Екатерина Валерьевна — к. б. н.

 Санкт-Петербург

 WOS Researcher ID: AAE-4032-2022.

Scopus Author ID: 57211941455 

 Ekaterina V. Nikitina — Ph. D. in biology

St. Petersburg

WOS Researcher ID: AAE-4032-2022.

Scopus Author ID: 57211941455 

https://orcid.org/0000-0002-0170-6975ЦветковаИ. А.TsvetkovaI. A.

 Цветкова Ирина Анатольевна — к. б. н.

Санкт-Петербург

WOS Researcher ID: F-9426-2017.

Scopus Author ID: 57197832461 

 Irina A.Tsvetkova — Ph. D. in biology

St. Petersburg

 WOS Researcher ID: F-9426-2017.

Scopus Author ID: 57197832461 

https://orcid.org/0000-0003-1419-9068КалиногорскаяО. С.KalinogorskayaO. S.

 Калиногорская Ольга Серафимовна — к. м. н. 

Санкт-Петербург

WOS Researcher ID: AAW-3832-2020.

Scopus Author ID: 56525317800 

 Olga S. Kalinogorskaya — Ph. D. in medicine

St. Petersburg

 WOS Researcher ID: AAW-3832-2020.

Scopus Author ID: 56525317800 

https://orcid.org/0000-0002-3480-8089ГостевВ. В.GostevV. V.

 Гостев Владимир Валерьевич — к. б. н.

Санкт-Петербург

WOS Researcher ID: P-1949-2016.

Scopus Author ID: 55614534400 

 Vladimir V. Gostev — Ph. D. in biology

St. Petersburg

WOS Researcher ID: P-1949-2016.

Scopus Author ID: 55614534400 

https://orcid.org/0000-0003-3419-3659БелановС. С.BelanovS. S.

 Беланов Сергей Сергеевич — к. б. н.,

Хельсинки

 Sergey Belanov — Ph.D. in biology

 Helsinki 

https://orcid.org/0000-0002-1519-5299МоховА. С.MokhovA. S.

Мохов Алексей Сергеевич

Санкт-Петербург

 WOS Researcher ID: AAV-2943-2021.

Scopus Author ID: 57206484035

 Alexey S. Mokhov

 St. Petersburg

 WOS Researcher ID: AAV-2943-2021.

Scopus Author ID: 57206484035 

КалисниковаЕ. Л.KalisnikovaE. L.

 Калисникова Екатерина Леонидовна

Санкт-Петербург 

 Ekaterina L. Kalisnikova

St. Petersburg 

https://orcid.org/0000-0002-3963-0144АгеевецВ. А.AgeevetsV. A.

 Агеевец Владимир Андреевич — к. б. н.

Санкт-Петербург

WOS Researcher ID: F-9282-2017

Scopus Author ID: 55949608900 

 Vladimir A. Ageevets — Ph. D. in biology

Saint Petersburg

WOS Researcher ID: F-9282-2017.

Scopus Author ID: 55949608900 

ГладинД. П.GladinD. P.

 Гладин Дмитрий Павлович — к. м. н., доцент, и. о. заведующего кафедрой микробиологии, вирусологии и иммунологии

Санкт-Петербург

Scopus Author ID: 6603374770 

 Dmitry P. Gladin — Ph. D. in medicine

Saint Petersburg

Scopus Author ID: 6603374770 

https://orcid.org/0000-0003-3550-7875СидоренкоС. В.SidorenkoS. V.

 Сидоренко Сергей Владимирович — д. м. н., профессор

 WOS Researcher ID: E-5870-2011.

Scopus Author ID: 7102484509 

 ул Профессора Попова, д. 9, г. Санкт-Петербург, 197022.

 

 Sergey V. Sidorenko — D. Sc. in medicine, Professor

St. Petersburg

WOS Researcher ID: E-5870-2011.

Scopus Author ID: 7102484509 

sidorserg@gmail.comФГБУ «Детский научно-клинический центр инфекционных болезней Федерального медико-биологического агентства»РоссияPediatric Research and Clinical Center for Infectious Diseases under the Federal Medical Biological AgencyRussian FederationФГБУ «Детский научно-клинический центр инфекционных болезней Федерального медико-биологического агентства»; ФГБОУ ВО «Северо-Западный государственный медицинский университет им. И. И. Мечникова» Минздрава РоссииРоссияPediatric Research and Clinical Center for Infectious Diseases under the Federal Medical Biological Agency; North-Western State Medical University named after I. I. Mechnikov of the Ministry of Health of the Russian FederationRussian FederationУниверситет Хельсинки, Институт БиотехнологииФинляндияUniversity of Helsinki, Institute of BiotechnologyFinlandФГБОУ ВО «Северо-Западный государственный медицинский университет им. И. И. Мечникова» Минздрава РоссииРоссияNorth-Western State Medical University named after I. I. Mechnikov of the Ministry of Health of the Russian FederationRussian FederationФГБОУ ВО «Санкт-Петербургский государственный педиатрический медицинский университет» Минздрава РоссииРоссияSt. Petersburg State Pediatric Medical University of the Ministry of Healthcare of the Russian FederationRussian Federation2021150320226611-121824Copyright © Никитина Е.В., Цветкова И.А., Калиногорская О.С., Гостев В.В., Беланов С.С., Мохов А.С., Калисникова Е.Л., Агеевец В.А., Гладин Д.П., Сидоренко С.В., 20222022Никитина Е.В., Цветкова И.А., Калиногорская О.С., Гостев В.В., Беланов С.С., Мохов А.С., Калисникова Е.Л., Агеевец В.А., Гладин Д.П., Сидоренко С.В.Nikitina E.V., Tsvetkova I.A., Kalinogorskaya O.S., Gostev V.V., Belanov S.S., Mokhov A.S., Kalisnikova E.L., Ageevets V.A., Gladin D.P., Sidorenko S.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.antibiotics-chemotherapy.ru/jour/article/view/875

В работе представлены оптимизированные методы ПЦР- и сиквенс-типирования Streptococcus pneumoniae. С использованием оптимизированных методов проанализирован серотиповой состав пневмококков, выделенных от детей в возрасте до 5 лет с инфекциями верхних дыхательных путей. В период с 2016 по 2021 гг. отмечено снижение частоты серотипов, входящих в 13-валентную конъюгированную пневмококковую вакцину (ПКВ13) с 94,1 до 25,8%, преимущественно за счёт серогруппы 6ABCD и серотипа 19F. Охват циркулирующих у детей серотипов вакцинами ПКВ15 и ПКВ20 составил в 2021 г. 28,1 и 41,6%, соответственно. За период исследования наиболее существенно возросло количество невакцинных серогрупп 11AD и 15AF, а также серотипов, не детектируемых в рамках данного протокола капсульного ПЦР-типирования.

The paper presents optimized methods for PCR and sequence typing of Streptococcus pneumoniae. The serotype composition of pneumococci isolated from children under 5 years of age with infections of the upper respiratory tract was analyzed using optimized methods. Between 2016 and 2021, there was a decrease in the frequency of serotypes included in the pneumococcal 13-valent conjugate vaccine (PCV13) from 94.1 to 25.8%, mainly due to the 6ABCD serogroup and the 19F serotype. The coverage of serotypes circulating in children with PCV15 and PCV20 vaccines was 28.1% and 41.6% in 2021, respectively. During the study period, the number of non-vaccine serogroups 11AD and 15AF, as well as serotypes that are not detected under this capsular PCR typing protocol, increased most significantly.

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The authors declare that there are no conflicts of interest present.