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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">antibiotics</journal-id><journal-title-group><journal-title xml:lang="ru">Антибиотики и Химиотерапия</journal-title><trans-title-group xml:lang="en"><trans-title>Antibiot Khimioter = Antibiotics and Chemotherapy</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">0235-2990</issn><publisher><publisher-name>ООО «Издательство ОКИ»</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.37489/0235-2990-2022-67-3-4-29-35</article-id><article-id custom-type="elpub" pub-id-type="custom">antibiotics-910</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>ЭКСПЕРИМЕНТАЛЬНЫЕ СТАТЬИ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>Experimental Research</subject></subj-group></article-categories><title-group><article-title>Оценка антибактериальной активности экстрактов коры Betula pendula (березы повислой) в отношении уропатогенных микроорганизмов</article-title><trans-title-group xml:lang="en"><trans-title>Evaluation of the Antibacterial Activity of Extracts of Betula Pendula (Silver Birch) Bark Against Uropathogenic Microorganisms</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-3871-9200</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Чапурин</surname><given-names>Ю. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Chapurin</surname><given-names>Y. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p> Чапурин Юрий Вадимович — студент, кафедра микробиологии им. В. С. Киктенко, Медицинский институт</p><p>ул. Миклухо-Маклая, 6, г. Москва,  117198.</p></bio><bio xml:lang="en"><p> Yuri V. Chapurin — student, Department of Microbiologynamed after V. S. Kiktenko </p><p>6 Miklukho-Maklaya st., Moscow, 117198 </p></bio><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-9626-9247</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Мбарга</surname><given-names>М. Д. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Mbarga</surname><given-names>M. J. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p> Мбарга Манга Джозеф Арсен — аспирант, лаборант, кафедра микробиологии им. В. С. Киктенко, Медицинский институт </p><p>Scopus ID: 57222221732 </p><p>ул. Миклухо-Маклая, 6, г. Москва,  117198.</p></bio><bio xml:lang="en"><p> Mbarga Manga Joseph Arsen — postgraduate student, laboratory assistant, Department of Microbiology named after V. S. Kiktenko </p><p> Scopus ID: 57222221732 </p><p>6 Miklukho-Maklaya st., Moscow, 117198 </p></bio><email xlink:type="simple">josepharsenembarga@yahoo.fr</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-4981-0149</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Сенягин</surname><given-names>А. Н.</given-names></name><name name-style="western" xml:lang="en"><surname>Senyagin</surname><given-names>A. N.</given-names></name></name-alternatives><bio xml:lang="ru"><p> Сенягин Александр Николаевич — аспирант, ассистент,кафедра микробиологии им. В.С. Киктенко, Медицинский институт</p><p>Scopus ID : 57217030559 </p><p>ул. Миклухо-Маклая, 6, г. Москва,  117198.</p></bio><bio xml:lang="en"><p> Alexander N. Senyagin — postgraduate Student, assistant, Department of Microbiology named after V. S. Kiktenko</p><p>Scopus ID: 57217030559 </p><p>6 Miklukho-Maklaya st., Moscow, 117198 </p></bio><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0003-4099-2967</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Подопригора</surname><given-names>И. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Podoprigora</surname><given-names>I. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p> Подопригора Ирина Викторовна — к. м. н., заведующийкафедрой, кафедра микробиологии им. В. С. Киктенко,Медицинский институт</p><p>Scopus ID: 57205375027 </p><p>ул. Миклухо-Маклая, 6, г. Москва,  117198.</p></bio><bio xml:lang="en"><p>Irina V. Podoprigora — Ph. D. in medicine</p><p>Scopus ID: 57205375027</p><p>6 Miklukho-Maklaya st., Moscow, 117198 </p></bio><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-9468-6589</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Манар</surname><given-names>Рехайлия</given-names></name><name name-style="western" xml:lang="en"><surname>Manar</surname><given-names>Rehailia</given-names></name></name-alternatives><bio xml:lang="ru"><p> Рехайлия Манар — аспирант, кафедра агробиотехнологии, Аграрно-технологический институт</p><p>ул. Миклухо-Маклая, 6, г. Москва,  117198.</p></bio><bio xml:lang="en"><p> Manar Rehailia — postgraduate student, Department of Agrobiotechnology </p><p>6 Miklukho-Maklaya st., Moscow, 117198 </p></bio><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>Российский университет дружбы народов</institution><country>Россия</country></aff><aff xml:lang="en"><institution>RUDN University</institution><country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2022</year></pub-date><pub-date pub-type="epub"><day>05</day><month>08</month><year>2022</year></pub-date><volume>67</volume><issue>3-4</issue><fpage>29</fpage><lpage>35</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Чапурин Ю.В., Мбарга М.А., Сенягин А.Н., Подопригора И.В., Манар Р., 2022</copyright-statement><copyright-year>2022</copyright-year><copyright-holder xml:lang="ru">Чапурин Ю.В., Мбарга М.А., Сенягин А.Н., Подопригора И.В., Манар Р.</copyright-holder><copyright-holder xml:lang="en">Chapurin Y.V., Mbarga M.A., Senyagin A.N., Podoprigora I.V., Manar R.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://www.antibiotics-chemotherapy.ru/jour/article/view/910">https://www.antibiotics-chemotherapy.ru/jour/article/view/910</self-uri><abstract><sec><title>Цель</title><p>Цель: оценить антибактериальную активность водного и спиртового экстрактов коры Betula pendula (березы повислой) в отношении различных микроорганизмов, вызывающих инфекции мочевыводящих путей.</p></sec><sec><title>Материал и методы</title><p>Материал и методы. Были протестированы водный и этанольный экстракты биологически активных соединений из коры Betula pendula в отношении десяти клинических уропатогенных штаммов (грамположительные бактерии — Kocuria rhizophila 1542, Staphylococcus simulans 5882, Enterococcus avium 1669, Enterococcus faecalis 5960 и Corynebacterium spp. 1638; грамотрицательные бактерии — Enterococcus cloacae, Morganella morganii 6392, Escherichia coli 1449, Seratia mansescens 6441 и Achromobacter xylosoxidans 4892). Staphylococcus aureus ATCC 6538 и Escherichia coli ATCC 25922 использовали как референс-стандарты грамположительных и грамотрицательных микроорганизмов. Чувствительность опытных штаммов к антибиотику оценивали диско-диффузионным методом Кирби–Бауэра, в то время как антибактериальную активность полученных экстрактов оценивали с использованием метода диффузии в агар. Также определили минимальную подавляющую концентрацию (МПК) и минимальную бактерицидную концентрацию (МБК) методом серийных разведений.</p></sec><sec><title>Результаты</title><p>Результаты. Кора B.pendula содержала 78,85% сухого вещества. Объёмный выход водного (AE) и этанольного экстрактов (EE) составлял 74,66% и 86,66% (об./об.), соответственно, тогда как их массовые выходы составляли 6,59% и 10,65% (мас./мас.). K.rizophilia 1542 и Corynebacterium spp. 1638 были наиболее устойчивыми бактериями с индексом множественной лекарственной устойчивости 0,45. AE и EE были активны в отношении всех тестируемых микроорганизмов. МПК AE варьировала от 8 до 32 мг / мл, тогда как МПК — от 2 до 16 мг / мл.</p></sec><sec><title>Заключение</title><p>Заключение. Водный экстракт коры Betula pendula проявляет слабую антимикробную активность, тогда как этанольный экстракт проявляет более выраженную антимикробную активность, но обладает бактериостатическим действием.</p></sec></abstract><trans-abstract xml:lang="en"><p>The aim of this work was to evaluate the antibacterial activity of the aqueous and ethanolic extracts of Betula pendula (silver birch) bark against various microorganisms causing urinary tract infections.</p><sec><title>Material and methods</title><p>Material and methods. Aqueous and ethanol extracts of biologically active compounds from Betula pendula bark were tested against ten clinical uropathogenic strains (gram-positive bacteria — Kocuria rhizophila 1542, Staphylococcus simulans 5882, Enterococcus avium 1669, Enterococcus faecalis 5960, and Corynebacterium spp. 1638; gram-negative bacteria — Enterococcus cloacae, Morganella morganii 6392, Escherichia coli 1449, Seratia mansescens 6441, and Achromobacter xylosoxidans 4892). Staphylococcus aureus ATCC 6538 and Escherichia coli ATCC 25922 were used as reference standards for Gram-positive and Gram-negative microorganisms. The sensitivity of the experimental strains to the antibiotic was evaluated by the Kirby–Bauer disk diffusion method, while the antibacterial activity of the obtained extracts was evaluated using the agar diffusion method. The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) were also determined via the serial dilution method.</p></sec><sec><title>Results</title><p>Results. The bark of B.pendula contained 78.85% of dry matter. The volume yield of the aqueous (AE) and ethanol extract (EE) was 74.66% and 86.66% (v/v), respectively, while their mass yields were 6.59% and 10.65% (w/w). K.rizophilia 1542 and Corynebacterium spp. 1638 were the most resistant bacteria with a multidrug resistance index of 0.45. AE and EE were active against all microorganisms tested. MIC of AE ranged from 8 to 32 mg/ml, while MIC of EE ranged from 2 to 16 mg/ml.</p></sec><sec><title>Conclusion</title><p>Conclusion. An aqueous extract of Betula pendula bark exhibits weak antimicrobial activity, while the ethanol extract exhibits a more pronounced antimicrobial activity, but has a bacteriostatic effect.</p></sec><sec><title> </title><p> </p></sec></trans-abstract><kwd-group xml:lang="ru"><kwd>Betula pendula</kwd><kwd>береза повислая</kwd><kwd>антибактериальное средство</kwd><kwd>инфекции мочевыводящих путей</kwd><kwd>растительные антибиотики</kwd></kwd-group><kwd-group xml:lang="en"><kwd>Betula pendula</kwd><kwd>silver birch</kwd><kwd>antibacterial agent</kwd><kwd>urinary tract infections</kwd><kwd>herbal antibiotics</kwd></kwd-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Motse D.F.K., Ngaba G.P., Foko L.P.K., Ebongue C.O., Adiogo D.D. Etiologic profile and sensitivity pattern of germs responsible for urinary tract infection among under-five children in Douala, Cameroon: a Hospital Based Study. Avicenna J Clin Microbiol Infect. 2019; 6 (2): 49–56. doi: 10.34172/ajcmi.2019.10.</mixed-citation><mixed-citation xml:lang="en">Motse D.F.K., Ngaba G.P., Foko L.P.K., Ebongue C.O., Adiogo D.D. Etiologic profile and sensitivity pattern of germs responsible for urinary tract infection among under-five children in Douala, Cameroon: a Hospital Based Study. Avicenna J Clin Microbiol Infect. 2019; 6 (2): 49–56. doi: 10.34172/ajcmi.2019.10.</mixed-citation></citation-alternatives></ref><ref id="cit2"><label>2</label><citation-alternatives><mixed-citation xml:lang="ru">Walsh C., Collyns T. The pathophysiology of urinary tract infections. Surgery (Oxf), 2020; 38 (4): 191–196. doi: 10.1016/j.mpsur.2017.03.007.</mixed-citation><mixed-citation xml:lang="en">Walsh C., Collyns T. The pathophysiology of urinary tract infections. Surgery (Oxf), 2020; 38 (4): 191–196. doi: 10.1016/j.mpsur.2017.03.007.</mixed-citation></citation-alternatives></ref><ref id="cit3"><label>3</label><citation-alternatives><mixed-citation xml:lang="ru">Abou Heidar N.F., Degheili J.A., Yacoubian A.A., Khauli R.B. Management of urinary tract infection in women: a practical approach for everyday practice. Urol Ann. 2019; 11 (4): 339. doi: 10.4103/UA.UA_104_19.</mixed-citation><mixed-citation xml:lang="en">Abou Heidar N.F., Degheili J.A., Yacoubian A.A., Khauli R.B. Management of urinary tract infection in women: a practical approach for everyday practice. Urol Ann. 2019; 11 (4): 339. doi: 10.4103/UA.UA_104_19.</mixed-citation></citation-alternatives></ref><ref id="cit4"><label>4</label><citation-alternatives><mixed-citation xml:lang="ru">Mbarga M.M.J., Andreevna S.L., Viktorovna P.I. Evaluation of apparent microflora and study of antibiotic resistance of coliforms isolated from the shells of poultry eggs in Moscow-Russia. J Adv Microbiol. 2020; 20 (4): 70–77. doi: 10.9734/jamb/2020/v20i430242.</mixed-citation><mixed-citation xml:lang="en">Mbarga M.M.J., Andreevna S.L., Viktorovna P.I. Evaluation of apparent microflora and study of antibiotic resistance of coliforms isolated from the shells of poultry eggs in Moscow-Russia. J Adv Microbiol. 2020; 20 (4): 70–77. doi: 10.9734/jamb/2020/v20i430242.</mixed-citation></citation-alternatives></ref><ref id="cit5"><label>5</label><citation-alternatives><mixed-citation xml:lang="ru">Суадкиа С., Подопригора И. В., Яшина Н. В., Саруханова Л. Е., Кравцов Э. Г. Антибиотикорезистентные уропатогенные Escherichia coli, выделенные от детей с врождёнными аномалиями развития мочевыделительной системы 7–8 (23–26). doi: 10.37489/0235-2990-2020-65-7-8-23-26.</mixed-citation><mixed-citation xml:lang="en">Suadkia S., Podoprigora I.V., Yashina N.V., Sarukhanova L.E., Kravtsov E.G. Antibiotic-resistant uropathogenic Escherichia coli isolated from children with birth defects of the urinary system. Antibiotiki i Khimioter = Antibiotics and Chemotherapy. 2020; 65 (7–8): 23–26. doi: 10.37489/0235-2990-2020-65-7-8-23-26. (in Russian)</mixed-citation></citation-alternatives></ref><ref id="cit6"><label>6</label><citation-alternatives><mixed-citation xml:lang="ru">Wojnicz D., Kucharska A.Z., Sokół-Łętowska A., Kicia M., Tichaczek-Goska D. Medicinal plants extracts affect virulence factors expression and biofilm formation by the uropathogenic Escherichia coli. Urol Res. 2012; 40 (6): 683–97. doi: 10.1007/s00240-012-0499-6.</mixed-citation><mixed-citation xml:lang="en">Wojnicz D., Kucharska A.Z., Sokół-Łętowska A., Kicia M., Tichaczek-Goska D. Medicinal plants extracts affect virulence factors expression and biofilm formation by the uropathogenic Escherichia coli. Urol Res. 2012; 40 (6): 683–97. doi: 10.1007/s00240-012-0499-6.</mixed-citation></citation-alternatives></ref><ref id="cit7"><label>7</label><citation-alternatives><mixed-citation xml:lang="ru">Manga M.J.A., Viktorovna P.I., Grigorievna V.E., Davares A.K., Sergeevna D.M. et al. Prolonged exposure to antimicrobials induces changes in susceptibility to antibiotics, biofilm formation and pathogenicity in Staphylococcus aureus. Journal of Pharmaceutical Research International, 2021; 33 (34B): 140–151. doi: 10.9734/jpri/2021/v33i34B31856.</mixed-citation><mixed-citation xml:lang="en">Manga M.J.A., Viktorovna P.I., Grigorievna V.E., Davares A.K., Sergeevna D.M. et al. Prolonged exposure to antimicrobials induces changes in susceptibility to antibiotics, biofilm formation and pathogenicity in  Staphylococcus aureus. Journal of Pharmaceutical Research International, 2021; 33 (34B): 140–151. doi: 10.9734/jpri/2021/v33i34B31856.</mixed-citation></citation-alternatives></ref><ref id="cit8"><label>8</label><citation-alternatives><mixed-citation xml:lang="ru">CLSI: Clinical &amp; Laboratory Standards Institute. Control methods. Biological and micro-biological factors: Determination of the sensitivity of microorganisms to antibacterial drugs. Federal Center for Sanitary and Epidemiological Surveillance of Ministry of Health of Russia; 2019.</mixed-citation><mixed-citation xml:lang="en">CLSI: Clinical &amp; Laboratory Standards Institute. Control methods. Biological and micro-biological factors: Determination of the sensitivity of microorganisms to antibacterial drugs. Federal Center for Sanitary and Epidemiological Surveillance of Ministry of Health of Russia; 2019.</mixed-citation></citation-alternatives></ref><ref id="cit9"><label>9</label><citation-alternatives><mixed-citation xml:lang="ru">Mondal A.H., Yadav D., Mitra S., Mukhopadhyay K. Biosynthesis of silver nanoparticles using culture supernatant of shewanella sp. Ary1 and their antibacterial activity. International Journal of Nanomedicine, 2020; 15: 8295. doi: 10.2147/IJN.S274535.</mixed-citation><mixed-citation xml:lang="en">Mondal A.H., Yadav D., Mitra S., Mukhopadhyay K. Biosynthesis of silver nanoparticles using culture supernatant of shewanella sp. Ary1 and their antibacterial activity. International Journal of Nanomedicine, 2020; 15: 8295. doi: 10.2147/IJN.S274535.</mixed-citation></citation-alternatives></ref><ref id="cit10"><label>10</label><citation-alternatives><mixed-citation xml:lang="ru">Arsene M.M.J., Podoprigora I.V., Davares A.K.L., Razan M., Das M. S., Senyagin A. N. Antibacterial activity of grapefruit peel extracts and greensynthesized silver nanoparticles, Veterinary World. 2021; 14 (5): 1330–1341. doi: 10.14202/vetworld.2021.1330-1341.</mixed-citation><mixed-citation xml:lang="en">Arsene M.M.J., Podoprigora I.V., Davares A.K.L., Razan M., Das M. S., Senyagin A. N. Antibacterial activity of grapefruit peel extracts and greensynthesized silver nanoparticles, Veterinary World. 2021; 14 (5): 1330–1341. doi: 10.14202/vetworld.2021.1330-1341.</mixed-citation></citation-alternatives></ref><ref id="cit11"><label>11</label><citation-alternatives><mixed-citation xml:lang="ru">Ezemokwe G.C., Aguiyi J.C., Chollom F.P. The antibacterial activity of aqueous and ethanolic leaf extracts of Balanites aegyptiaca (L.) Del plant on some selected clinical human pathogens. J. Adv. Microbiol. 2020; 20(10): 51–66. doi:10.9734/jamb/2020/v20i1030290.</mixed-citation><mixed-citation xml:lang="en">Ezemokwe G.C., Aguiyi J.C., Chollom F.P. The antibacterial activity of aqueous and ethanolic leaf extracts of Balanites aegyptiaca (L.) Del plant on some selected clinical human pathogens. J. Adv. Microbiol. 2020; 20(10): 51–66. doi:10.9734/jamb/2020/v20i1030290.</mixed-citation></citation-alternatives></ref><ref id="cit12"><label>12</label><citation-alternatives><mixed-citation xml:lang="ru">Ibrahim M.A., Emlee A.M. Anti-fungal study on aqueous and ethanolic leaves extracts of Piper sarmentosum. Matrix Sci. Pharm. 2020; 4 (1): 13.doi: 10.4103/MTSP.MTSP_3_20.</mixed-citation><mixed-citation xml:lang="en">Ibrahim M.A., Emlee A.M. Anti-fungal study on aqueous and ethanolic leaves extracts of Piper sarmentosum. Matrix Sci. Pharm. 2020; 4 (1): 13.doi: 10.4103/MTSP.MTSP_3_20.</mixed-citation></citation-alternatives></ref><ref id="cit13"><label>13</label><citation-alternatives><mixed-citation xml:lang="ru">Noshad M. Evaluation of the effect of aqueous and ethanolic extraction methods on extraction yield, phenolic compounds, and antioxidant and antimicrobial activity of Stachys schtschegleevii extract. Food Sci Technol. 2020; 17 (100): 117–125.</mixed-citation><mixed-citation xml:lang="en">Noshad M. Evaluation of the effect of aqueous and ethanolic extraction methods on extraction yield, phenolic compounds, and antioxidant and antimicrobial activity of Stachys schtschegleevii extract. Food Sci Technol. 2020; 17 (100): 117–125.</mixed-citation></citation-alternatives></ref><ref id="cit14"><label>14</label><citation-alternatives><mixed-citation xml:lang="ru">Gonfa T., Teketle S., Kiros T. Effect of extraction solvent on qualitative and quantitative analysis of major phyto-constituents and in-vitro antioxidant activity evaluation of Cadaba rotundifolia Forssk leaf extracts. Cogent Food Agric. 2020; 6 (1): 1853867. doi: 10.1080/23311932.2020.1853867.</mixed-citation><mixed-citation xml:lang="en">Gonfa T., Teketle S., Kiros T. Effect of extraction solvent on qualitative and quantitative analysis of major phyto-constituents and in-vitro antioxidant activity evaluation of Cadaba rotundifolia Forssk leaf extracts. Cogent Food Agric. 2020; 6 (1): 1853867. doi: 10.1080/23311932.2020.1853867.</mixed-citation></citation-alternatives></ref><ref id="cit15"><label>15</label><citation-alternatives><mixed-citation xml:lang="ru">Shrestha U.T., Shrestha S., Adhikari N. et al. Plasmid profiling and occurrence of β-lactamase enzymes in multidrug-resistant uropathogenic Escherichia coli in Kathmandu, Nepal. Infect Drug Resist. 2020; 13: 1905–1917. doi:10.2147/IDR.S250591.</mixed-citation><mixed-citation xml:lang="en">Shrestha U.T., Shrestha S., Adhikari N. et al. Plasmid profiling and occurrence of β-lactamase enzymes in multidrug-resistant uropathogenic Escherichia coli in Kathmandu, Nepal. Infect Drug Resist. 2020; 13: 1905–1917. doi:10.2147/IDR.S250591.</mixed-citation></citation-alternatives></ref><ref id="cit16"><label>16</label><citation-alternatives><mixed-citation xml:lang="ru">Onivogui G., Letsididi R., Diaby M., Wang L., Song Y. Influence of extraction solvents on antioxidant and antimicrobial activities of the pulp and seed of Anisophyllea laurina R. Br. ex Sabine fruits. Asian Pacific Journal of Tropical Biomedicine. 2015; 6(1): 20–25. doi:10.1016/j.apjtb.2015.09.023.</mixed-citation><mixed-citation xml:lang="en">Onivogui G., Letsididi R., Diaby M., Wang L., Song Y. Influence of extraction solvents on antioxidant and antimicrobial activities of the pulp and seed of Anisophyllea laurina R. Br. ex Sabine fruits. Asian Pacific Journal of  Tropical Biomedicine. 2015; 6(1): 20–25. doi:10.1016/j.apjtb.2015.09.023.</mixed-citation></citation-alternatives></ref><ref id="cit17"><label>17</label><citation-alternatives><mixed-citation xml:lang="ru">Al Farraj D.A., Abdel Gawwad M.R., Mehmood A., Alsalme A., Darwish N.M., Al-Zaqri N., Warad I. In-vitro antimicrobial activities of organic solvent extracts obtained from Dipcadi viride (L.) Moench. Journal of King Saud University — Science. 2020; 32 (2020): 1965–1968. doi:10.1016/j.jksus.2020.01.007.</mixed-citation><mixed-citation xml:lang="en">Al Farraj D.A., Abdel Gawwad M.R., Mehmood A., Alsalme A., Darwish N.M., Al-Zaqri N., Warad I. In-vitro antimicrobial activities of organic solvent extracts obtained from Dipcadi viride (L.) Moench. Journal of King Saud University — Science. 2020; 32 (2020): 1965–1968. doi:10.1016/j.jksus.2020.01.007.</mixed-citation></citation-alternatives></ref><ref id="cit18"><label>18</label><citation-alternatives><mixed-citation xml:lang="ru">Evbuomwan L., Chukwuka E.P., Obazenu E.I., Ilevbare L. Antibacterial activity of vernonia amygdalina leaf extracts against multidrug resistant bacterial isolates. J Appl Sci Environ Manage. 2018; 22 (1): 17–21. doi: 10.4314/jasem.v22i1.4.</mixed-citation><mixed-citation xml:lang="en">Evbuomwan L., Chukwuka E.P., Obazenu E.I., Ilevbare L. Antibacterial activity of vernonia amygdalina leaf extracts against multidrug resistant bacterial isolates. J Appl Sci Environ Manage. 2018; 22 (1): 17–21. doi: 10.4314/jasem.v22i1.4.</mixed-citation></citation-alternatives></ref><ref id="cit19"><label>19</label><citation-alternatives><mixed-citation xml:lang="ru">Navid M.H., Laszczyk-Lauer M.N., Reichling J., Schnitzler P. Pentacyclic triterpenes in birch bark extract inhibit early step of herpes simplex virus type 1 replication, Phytomedicine. 2014; 21 (11): 1273–1280. doi: 10.1016/j.phymed.2014.06.007.</mixed-citation><mixed-citation xml:lang="en">Navid M.H., Laszczyk-Lauer M.N., Reichling J., Schnitzler P. Pentacyclic triterpenes in birch bark extract inhibit early step of herpes simplex virus type 1 replication, Phytomedicine. 2014; 21 (11): 1273–1280. doi: 10.1016/j.phymed.2014.06.007.</mixed-citation></citation-alternatives></ref><ref id="cit20"><label>20</label><citation-alternatives><mixed-citation xml:lang="ru">Kuete V. Potential of Cameroonian plants and derived products against microbial infections: a review. Planta Med. 2010; 76: 1479–1491. doi:10.1055/s-0030-1250027.</mixed-citation><mixed-citation xml:lang="en">Kuete V. Potential of Cameroonian plants and derived products against microbial infections: a review. Planta Med. 2010; 76: 1479–1491. doi:10.1055/s-0030-1250027.</mixed-citation></citation-alternatives></ref></ref-list><fn-group><fn fn-type="conflict"><p>The authors declare that there are no conflicts of interest present.</p></fn></fn-group></back></article>
