antibioticsАнтибиотики и ХимиотерапияAntibiot Khimioter = Antibiotics and Chemotherapy0235-2990ООО «Издательство ОКИ»10.37489/0235-2990-2022-67-5-6-14-18antibiotics-924Research ArticleЭКСПЕРИМЕНТАЛЬНЫЕ СТАТЬИExperimental ResearchМолекулярная характеристика Neisseria meningitidis серогруппы W в Санкт-ПетербургеMolecular Сharacterization of Neisseria meningitidis Serogroup W in St. Petersburghttps://orcid.org/0000-0001-6093-7493МАРТЕНСЭ. А.MARTENSE. A.

Мартенс Эльвира Акрамовна — заведующий клинико-диагностической лабораторией, младший научный сотрудник, ФГБУ «Детский научно-клинический центр инфекционных болезней ФМБА России»; ассистент кафедры медицинской микробиологии ФГБОУ ВО СЗГМУ им. И. И. Мечникова Минздрава России.WOS Researcher ID: CAG-4447-2022Scopus Author ID: 57206470215

Санкт-Петербург

Elvira A. Martens — Head of the Clinical Diagnostic Laboratory, Junior Researcher, Pediatric Research and Clinical Center for Infectious Diseases under the Federal Medical Biological Agency; Assistant at the Department of Medical Microbiology North-Western State Medical University named after I. I. Mechnikov.WOS Researcher ID: CAG-4447-2022Scopus Author ID: 57206470215

Saint Petersburg

https://orcid.org/0000-0001-8071-3243ЖЕЛЕЗОВАЛ. И.ZHELEZOVAL. I.

Железова Людмила Ильинична — к. м. н., старший научный сотрудник отдела медицинской микробиологии и молекулярной эпидемиологииWOS Researcher ID: G-9662-2012Scopus Author ID: 6505716185

Санкт-Петербург

Lyudmila I. Zhelezova — Ph. D. in medicine, Pediatric Research and Clinical Center for Infectious Diseases. WOS Researcher ID: G-9662- 2012Scopus Author ID: 6505716185

Saint Petersburg

https://orcid.org/0000-0002-3480-8089ГОСТЕВВ. В.GOSTEVV. V.

Гостев Владимир Валерьевич — к. б. н., старший научный сотрудник отдела медицинской микробиологии и молекулярной эпидемиологии ФГБУ «Детский научно-клинический центр инфекционных болезней ФМБА России»; Доцент кафедры медицинской микробиологии ФГБОУ ВО СЗГМУ им. И. И. Мечникова Минздрава. WOS Researcher ID: P-1949-2016WOS Researcher ID: 55614534400

197022, г. Санкт-Петербург, ул Профессора Попова, д. 9

Vladimir V. Gostev — Ph. D. in biology, Pediatric Research and Clinical Center for Infectious Diseases under the Federal Medical Biological Agency, North-Western State Medical University named after I. I. Mechnikov. WOS Researcher ID: P-1949-2016WOS Researcher ID: 55614534400

Saint Petersburg

guestvv11@gmail.comЛИХОЛЕТОВАД. В.LIKHOLETOVAD. V.

Лихолетова Дарья Вадимовна — лаборант-исследователь отдела медицинской микробиологии и молекулярной эпидемиологии

Санкт-Петербург

Daria V. Likholetova — Laboratory researcher at the Department of Medical Microbiology and Molecular Epidemiology

Saint Petersburg

https://orcid.org/0000-0001-8666-6118ЗАХАРЕНКОС. М.ZAKHARENKOS. M.

Захаренко Сергей Михайлович — к. м. н., доцент, заместитель директораSCOPUS: 6701402980

Санкт-Петербург

Sergey M. Zakharenko — Ph. D. in medicine, associate professorSCOPUS: 6701402980.

Saint Petersburg

ФГБУ, «Детский научно-клинический центр по инфекционным болезням Федерального медико-биологического агентства»; ФГБОУ ВО «Северо-Западный государственный медицинский университет им. И. И. Мечникова»РоссияPediatric Research and Clinical Center for Infectious Diseases under the Federal Medical Biological Agency; North-Western State Medical University named after I. I. MechnikovRussian FederationФГБУ, «Детский научно-клинический центр по инфекционным болезням Федерального медико-биологического агентства»РоссияPediatric Research and Clinical Center for Infectious Diseases under the Federal Medical Biological AgencyRussian Federation202208092022675-61418Copyright © МАРТЕНС Э.А., ЖЕЛЕЗОВА Л.И., ГОСТЕВ В.В., ЛИХОЛЕТОВА Д.В., ЗАХАРЕНКО С.М., 20222022МАРТЕНС Э.А., ЖЕЛЕЗОВА Л.И., ГОСТЕВ В.В., ЛИХОЛЕТОВА Д.В., ЗАХАРЕНКО С.М.MARTENS E.A., ZHELEZOVA L.I., GOSTEV V.V., LIKHOLETOVA D.V., ZAKHARENKO S.M.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.antibiotics-chemotherapy.ru/jour/article/view/924

В исследование включено 14 изолятов Neisseria meningitidis серогруппы W, выделенных от больных генерализованными формами менингококковой инфекции в возрасте 0–17 лет в НИИДИ-ДНКЦИБ в период с 2009 г. по 2020 г., и от носителей. Изоляты от носителей (17–18 лет) были выделены в 2016–2019 гг. Идентификацию выделенных изолятов проводили методом MALDI-TOF масс-спектрометрии с использованием масс-спектрометра Microflex LT (Bruker Daltonics, Германия). Полногеномное секвенирование геномов Neisseria meningitidis было проведено на базе платформы Miseq Illumina, при этом была установлена их принадлежность к ST11 (сс11). Результаты проведённого полногеномного секвенирования изучаемых менингококков показали, что в Санкт-Петербурге имеются 2 группы субкластера, относящегося к хадж-кластеру. Кроме того, имеются ближайшие родственные связи с менингококками Англо-Французского и Шведского кластеров Хадж линии. 

The study included 14 isolates of Neisseria meningitidis serogroup W, isolated from patients with generalized forms of meningococcal infection aged 0–17 years at the Pediatric Research and Clinical Center for Infectious Diseases during the period from 2009 to 2020, as well as from carriers. The isolates from carriers (17–18 years old) were isolated in 2016–2019. The isolates were identified by MALDI-TOF mass spectrometry using a Microflex LT mass spectrometer (Bruker Daltonics, Germany). Whole genome sequencing of N. meningitidis was carried out on the basis of the Miseq Illumina platform; it was established that they belong to ST11 (cc11) sequence type. The results of the whole genome sequencing of the studied meningococci showed that there are 2 groups of the subcluster belonging to the Hajj cluster in St. Petersburg. In addition, they show close affinity with meningococci of the Anglo-French and Swedish clusters of the Hajj line.

Neisseria meningitidisменингококковая инфекцияполногеномное секвенированиеNeisseria meningitidismeningococcal infectionwhole genome sequencingReferencesMaiden M.C., Bygraves J.A., Feil E., Morelli G, Russell J.E., Urwin R., Zhang Q., Zhou J., Zurth K., Caugant D.A. et al. Multilocus sequence typing: a portable approach to the identification of clones within populations of pathogenic microorganisms. Proc Natl Acad Sci U S A 1998, 95: 3140–3145. doi:10.1073/pnas.95.6.3140.Maiden M.C., Bygraves J.A., Feil E., Morelli G, Russell J.E., Urwin R., Zhang Q., Zhou J., Zurth K., Caugant D.A. et al. Multilocus sequence typing: a portable approach to the identification of clones within populations of pathogenic microorganisms. Proc Natl Acad Sci U S A 1998, 95: 3140–3145. doi:10.1073/pnas.95.6.3140.Yazdankhah S.P., Kriz P., Tzanakaki G., Kremastinou J., Kalmusova J., Musilek M., Alvestad T., Jolley K.A., Wilson D.J., McCarthy N.D. et al. Distribution of serogroups and genotypes among disease-associated and carried isolates of Neisseria meningitidis from the Czech Republic, Greece, and Norway. J Clin Microbiol. 2004; 42: 5146–5153. doi:10.1128/JCM.42.11.5146-5153.2004.Yazdankhah S.P., Kriz P., Tzanakaki G., Kremastinou J., Kalmusova J., Musilek M., Alvestad T., Jolley K.A., Wilson D.J., McCarthy N.D. et al. Distribution of serogroups and genotypes among disease-associated and carried isolates of Neisseria meningitidis from the Czech Republic, Greece, and Norway. J Clin Microbiol. 2004; 42: 5146–5153. doi:10.1128/JCM.42.11.5146-5153.2004.Stephens D.S., Greenwood B., Brandtzaeg P. Epidemic meningitis, meningococcaemia, and Neisseria meningitidis. Lancet. 2007; 369: 2196–2210. doi:10.1016/S0140-6736(07)61016-2.Stephens D.S., Greenwood B., Brandtzaeg P. Epidemic meningitis, meningococcaemia, and Neisseria meningitidis. Lancet. 2007; 369: 2196–2210. doi:10.1016/S0140-6736(07)61016-2.Daugla D.M., Gami J.P., Gamougam K., Naibei N., Mbainadji L., Narbe M., Toralta J., Kodbesse B., Ngadoua C., Coldiron M.E. et al. Effect of a serogroup A meningococcal conjugate vaccine (PsA-TT) on serogroup A meningococcal meningitis and carriage in Chad: a community study [corrected]. Lancet. 2014; 383: 40–47. doi:10.1016/S0140-6736(13)61612-8. Epub 2013 Sep 12.Daugla D.M., Gami J.P., Gamougam K., Naibei N., Mbainadji L., Narbe M., Toralta J., Kodbesse B., Ngadoua C., Coldiron M.E. et al. Effect of a serogroup A meningococcal conjugate vaccine (PsA-TT) on serogroup A meningococcal meningitis and carriage in Chad: a community study [corrected]. Lancet. 2014; 383: 40–47. doi:10.1016/S0140-6736(13)61612-8. Epub 2013 Sep 12.Gamougam K., Daugla D.M., Toralta J., Ngadoua C., Fermon F, Page AL, Djingarey M.H., Caugant D.A., Manigart O., Trotter C.L. et al. Continuing effectiveness of serogroup A meningococcal conjugate vaccine, Chad, 2013. Emerg Infect Dis. 2015; 21: 115–118.Gamougam K., Daugla D.M., Toralta J., Ngadoua C., Fermon F, Page AL, Djingarey M.H., Caugant D.A., Manigart O., Trotter C.L. et al. Continuing effectiveness of serogroup A meningococcal conjugate vaccine, Chad, 2013. Emerg Infect Dis. 2015; 21: 115–118.Tzeng Y.L., Stephens D.S. A Narrative Review of the W, X, Y, E, and NG of Meningococcal Disease: Emerging Capsular Groups, Pathotypes, and Global Control. Microorganisms. 2021, 9 (3): 519. doi:10.3390/microorganisms9030519.Tzeng Y.L., Stephens D.S. A Narrative Review of the W, X, Y, E, and NG of Meningococcal Disease: Emerging Capsular Groups, Pathotypes, and Global Control. Microorganisms. 2021, 9 (3): 519. doi:10.3390/microorganisms9030519.Caugant D.A., Brynildsrud O.B. Neisseria meningitidis: using genomics to understand diversity, evolution and pathogenesis. Nat Rev Microbiol. 2020; 18: 84–96. doi:10.1038/s41579-019-0282-6.Caugant D.A., Brynildsrud O.B. Neisseria meningitidis: using genomics to understand diversity, evolution and pathogenesis. Nat Rev Microbiol. 2020; 18: 84–96. doi:10.1038/s41579-019-0282-6.Lucidarme J., Hill D.M., Bratcher H.B., Gray S.J., du Plessis M., Tsang R.S., Vazquez J.A., Taha M.K., Ceyhan M., Efron A.M. et al. Genomic resolution of an aggressive, widespread, diverse and expanding meningococcal serogroup B, C and W lineage. J Infect 2015, 71: 544–552.Lucidarme J., Hill D.M., Bratcher H.B., Gray S.J., du Plessis M., Tsang R.S., Vazquez J.A., Taha M.K., Ceyhan M., Efron A.M. et al. Genomic resolution of an aggressive, widespread, diverse and expanding meningococcal serogroup B, C and W lineage. J Infect 2015, 71: 544–552.Caugant D.A., Brynildsrud O.B. Neisseria meningitidis: using genomics to understand diversity, evolution and pathogenesis. Nat Rev Microbiol 2020, 18: 84–96. doi:10.1038/s41579-019-0282-6.Caugant D.A., Brynildsrud O.B. Neisseria meningitidis: using genomics to understand diversity, evolution and pathogenesis. Nat Rev Microbiol 2020, 18: 84–96. doi:10.1038/s41579-019-0282-6.Mayer L.W., Reeves M.W., Al-Hamdan N., Sacchi C.T., Taha M.K., Ajello G.W., Schmink S.E., Noble C.A., Tondella M.L., Whitney A.M. et al. Outbreak of W135 meningococcal disease in 2000: not emergence of a new W135 strain but clonal expansion within the electophoretic type-37 complex. J Infect Dis. 2002; 185: 1596–1605. doi:10.1086/340414.Mayer L.W., Reeves M.W., Al-Hamdan N., Sacchi C.T., Taha M.K., Ajello G.W., Schmink S.E., Noble C.A., Tondella M.L., Whitney A.M. et al. Outbreak of W135 meningococcal disease in 2000: not emergence of a new W135 strain but clonal expansion within the electophoretic type-37 complex. J Infect Dis. 2002; 185: 1596–1605. doi:10.1086/340414.Fazio C., Neri A., Vacca P., Ciammaruconi A., Arghittu M., Barbui A.M., Vocale C., Bernaschi P., Isola P., Galanti I.A. et al. Cocirculation of Hajj and non-Hajj strains among serogroup W meningococci in Italy, 2000 to 2016. Euro Surveill. 2019; 24 (4): 1800183. doi:10.2807/1560-7917.ES.2019.24.4.1800183.Fazio C., Neri A., Vacca P., Ciammaruconi A., Arghittu M., Barbui A.M., Vocale C., Bernaschi P., Isola P., Galanti I.A. et al. Cocirculation of Hajj and non-Hajj strains among serogroup W meningococci in Italy, 2000 to 2016. Euro Surveill. 2019; 24 (4): 1800183. doi:10.2807/1560-7917.ES.2019.24.4.1800183.Mustapha M.M., Marsh J.W., Harrison L.H. Global epidemiology of capsular group W meningococcal disease (1970–2015): Multifocal emergence and persistence of hypervirulent sequence type (ST)-11 clonal complex. Vaccine 2016, 34: 1515–1523. doi:10.1016/j.vaccine.2016.02.014.Mustapha M.M., Marsh J.W., Harrison L.H. Global epidemiology of capsular group W meningococcal disease (1970–2015): Multifocal emergence and persistence of hypervirulent sequence type (ST)-11 clonal complex. Vaccine 2016, 34: 1515–1523. doi:10.1016/j.vaccine.2016.02.014.Lucidarme J., Scott K.J., Ure R., Smith A.J., Lindsay D.S.J., Stenmark B., Jacobsson S., Fredlund H., Cameron J.C., Smith-Palmer A. et al. An international invasive meningococcal disease outbreak due to a novel and rapidly expanding serogroup W strain, Scotland and Sweden, July to August 2015. Euro Surveil. 2016; 21 (45): 30395. doi:10.2807/1560-7917.ES.2016.21.45.30395.Lucidarme J., Scott K.J., Ure R., Smith A.J., Lindsay D.S.J., Stenmark B., Jacobsson S., Fredlund H., Cameron J.C., Smith-Palmer A. et al. An international invasive meningococcal disease outbreak due to a novel and rapidly expanding serogroup W strain, Scotland and Sweden, July to August 2015. Euro Surveil. 2016; 21 (45): 30395. doi:10.2807/1560-7917.ES.2016.21.45.30395.Ladhani S.N., Beebeejaun K., Lucidarme J., Campbell H., Gray S., Kaczmarski E., Ramsay M.E., Borrow R. Increase in Endemic Neisseria meningitidis Capsular Group W Sequence Type 11 Complex Associated With Severe Invasive Disease in England and Wales. Clin Infect Dis. 2014; 60 (4): 578–585. doi:10.1093/cid/ciu881. Epub 2014 Nov 10.Ladhani S.N., Beebeejaun K., Lucidarme J., Campbell H., Gray S., Kaczmarski E., Ramsay M.E., Borrow R. Increase in Endemic Neisseria meningitidis Capsular Group W Sequence Type 11 Complex Associated With Severe Invasive Disease in England and Wales. Clin Infect Dis. 2014; 60 (4): 578–585. doi:10.1093/cid/ciu881. Epub 2014 Nov 10.Lemos A.P., Gorla M.C., de Moraes C., Willemann M.C.A., Sacchi C.T., Fukasawa L.O., Camargo C.H., Barreto G., Rodrigues D.S., Gonçalves M.G. et al. Emergence of Neisseria meningitidis W South American sublineage strain variant in Brazil: disease and carriage. J Med Microbiol. 2022; 71 (2). doi:10.1099/jmm.0.001484.Lemos A.P., Gorla M.C., de Moraes C., Willemann M.C.A., Sacchi C.T., Fukasawa L.O., Camargo C.H., Barreto G., Rodrigues D.S., Gonçalves M.G. et al. Emergence of Neisseria meningitidis W South American sublineage strain variant in Brazil: disease and carriage. J Med Microbiol. 2022; 71 (2). doi:10.1099/jmm.0.001484.Wick R.R., Judd L.M., Gorrie C.L., Holt K.E. Unicycler: Resolving bacterial genome assemblies from short and long sequencing reads. PLoS Comput Biol. 2017; 13:e1005595. doi:10.1371/journal.pcbi.1005595.Wick R.R., Judd L.M., Gorrie C.L., Holt K.E. Unicycler: Resolving bacterial genome assemblies from short and long sequencing reads. PLoS Comput Biol. 2017; 13:e1005595. doi:10.1371/journal.pcbi.1005595.Seemann T. Prokka: rapid prokaryotic genome annotation. Bioinformatics. 2014; 30: 2068–2069. doi:10.1093/bioinformatics/btu153Seemann T. Prokka: rapid prokaryotic genome annotation. Bioinformatics. 2014; 30: 2068–2069. doi:10.1093/bioinformatics/btu153Gardner S.N., Slezak T., Hall B.G. kSNP3.0: SNP detection and phylogenetic analysis of genomes without genome alignment or reference genome. Bioinformatics. 2015; 31 (17): 2877–2878. doi:10.1093/bioinformatics/btv271.Gardner S.N., Slezak T., Hall B.G. kSNP3.0: SNP detection and phylogenetic analysis of genomes without genome alignment or reference genome. Bioinformatics. 2015; 31 (17): 2877–2878. doi:10.1093/bioinformatics/btv271.https://pubmlst.org/https://pubmlst.org/

The authors declare that there are no conflicts of interest present.