antibioticsАнтибиотики и ХимиотерапияAntibiot Khimioter = Antibiotics and Chemotherapy0235-2990ООО «Издательство ОКИ»10.37489/0235-2990-2023-68-1-2-4-10antibiotics-997Research ArticleЭКСПЕРИМЕНТАЛЬНЫЕ ИССЛЕДОВАНИЯEXPERIMENTAL STUDIESЭкспресс- анализ воздействия аминогликозидов на бактерии с помощью сенсорной системы на основе пьезоэлектрического резонатора с поперечным электрическим полемRapid analysis of the effect of aminoglycosides on bacteria by using a sensor system based on a piezoelectric resonator with a lateral electric fieldГулийО. И.GuliyО. I.

Гулий Ольга Ивановна — д. б. н., профессор, ведущий научный сотрудник лаборатории биохимии

пр. Энтузиастов, ИБФРМ, г. Саратов, 410049

Olga I. Guliy — D. Sc. in Biology, Professor, Leading researcher Biochemistry Laboratory

13 Entuziastov ave., Institute of Biochemistry and Physiology of Plants and Microorganisms, Saratov, 410049

Saratov

guliy_olga@mail.ruЗайцевБ. Д.ZaitsevB. D.

Зайцев Борис Давыдович — д. ф.-м. н., профессор, главный научный сотрудник лаборатории физической акустики

Саратов

 

Boris D. Zaitsev — D. Sc. in Physical and Mathematical Sciences, Chief Researcher of the Laboratory of Physical Acoustics

Saratov

 

АлсовэйдиА.К.М.AlsowaidiA. К. М.

Алсовэйди Али Кадхим Мохаммед — аспирант очной формы обучения

Саратов

Ali Kadhim Mohammed Alsowaidi — full-time Ph. D. student

Saratov

КараваеваО. А.KaravaevaО. А.

Караваева Ольга Александровна — к. б. н., научный сотрудник лаборатории биохимии

Саратов

Olga A. Karavaeva — Ph. D. in Biology, Researcher of Biochemistry Laboratory

Saratov

СемёновА. П.SemyonovA. P.

Семёнов Александр Павлович — к. ф.-м. н., старший научный сотрудник лаборатории физической акустики

Саратов

Alexander P. Semyonov — Ph. D. in Physical and Mathematical Sciences, Senior researcher of the Laboratory of Physical Acoustics

Saratov

БородинаИ. А.BorodinaI. A.

Бородина Ирина Анатольевна — к. ф.-м. н., ведущий научный сотрудник лаборатории физической акустики

Саратов

Irina A. Borodina — Ph. D. in Physical and Mathematical Sciences, Leading Researcher of the Laboratory of Physical Acoustics

Saratov

Институт биохимии и физиологии растений и микроорганизмов, ФИЦ «Саратовский научный центр РАН» (ИБФРМ РАН)РоссияInstitute of Biochemistry and Physiology of Plants and Microorganisms of the Russian Academy of Sciences – Subdivision of the Federal State Budgetary Research Institution Saratov Federal Scientific Centre of the Russian Academy of Sciences (IBPPM RAS)Russian FederationИнститут радиотехники и электроники им. В. А. Котельникова РАН, Саратовский филиалРоссияSaratov Branch of the Kotelnikov Institute of Radio Engineering and Electronics, Russian Academy of Sciences, Saratov BranchRussian FederationСаратовский национальный исследовательский государственный университет им. Н. Г. ЧернышевскогоРоссияSaratov State UniversityRussian FederationИнститут биохимии и физиологии растений и микроорганизмов, ФИЦ «Саратовский научный центр РАН» (ИБФРМ РАН)РоссияInstitute of Biochemistry and Physiology of Plants and Microorganisms of the Russian Academy of Sciences — Subdivision of the Federal State Budgetary Research Institution Saratov Federal Scientific Centre of the Russian Academy of Sciences (IBPPM RAS)Russian FederationИнститут радиотехники и электроники им. В. А. Котельникова РАН, Саратовский филиалРоссияSaratov Branch of the Kotelnikov Institute of Radio Engineering and Electronics, Russian Academy of Sciences, Saratov BranchRussian Federation202329042023681-2410Copyright © Гулий О.И., Зайцев Б.Д., Алсовэйди А., Караваева О.А., Семёнов А.П., Бородина И.А., 20232023Гулий О.И., Зайцев Б.Д., Алсовэйди А., Караваева О.А., Семёнов А.П., Бородина И.А.Guliy О.I., Zaitsev B.D., Alsowaidi A., Karavaeva О.А., Semyonov A.P., Borodina I.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.antibiotics-chemotherapy.ru/jour/article/view/997

Показана возможность экспресс-анализа воздействия аминогликозидных антибиотиков на бактерии (Escherichia coli) с помощью сенсорной системы на основе пьезоэлектрического резонатора с поперечным электрическим полем с диапазоном рабочих частот 6–7 МГц. Для экспериментов использовали штаммы бактерий E.coli как чувствительные, так и устойчивые к канамицину. При оценке воздействия канамицина на бактерии использовали изменение модуля электрического импеданса резонатора в качестве аналитического сигнала. Установлено, что критерием чувствительности бактерий к канамицину является изменение модуля электрического импеданса датчика после воздействии антибиотика на бактерии на любой частоте вблизи резонанса. Датчик обладает высокой чувствительностью и позволяет проводить диагностику антимикробной восприимчивости бактерий в течение 7–9 мин.

The possibility of express analysis of the effect of aminoglycoside antibiotics on bacteria (Escherichia coli) using a sensor system based on a piezoelectric resonator with a lateral electric field with an operating frequency range of 6–7 MHz is shown. E.coli strains, both sensitive and resistant to kanamycin were used for the experiments. During evaluating the kanamycin effect on bacteria, the change in the electrical impedance modulus of the resonator was used as an analitical signal. It has been established that the criterion for the antibiotic bacteria sensitivity is the change in the modulus of the sensor electrical impedance after antibiotic exposure on bacteria at any frequency near the resonance. The sensor is highly sensitive and allows diagnosing the antimicrobial susceptibility of bacteria within 7–9 minutes.

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The authors declare that there are no conflicts of interest present.