Study of Molecular Parameters and Antibacterial Activity of a Peptide Preparation Derived from Human Leukocytes
https://doi.org/10.37489/0235-2990-2023-68-9-10-20-24
Abstract
Background. The study of new antimicrobial substances isolated from the living organism tissues is of particular importance for medicine and the biopharmaceutical industry. Leukocyte peptides can act as an alternative to traditional antibiotics to overcome the bacterial antibiotic resistance problem.
Aim. Evaluation of molecular weight and biological activity of ultrasound-derived leukocyte peptides.
Methods. Separation of leukocyte peptide complexes into particular fractions by reverse phase chromatography and evaluation of their antibacterial activity by serial dilution and diffusion methods.
Results. Studies have shown that one peptide fraction with a molecular weight of 440–570 Da has pronounced antibacterial activity.
Conclusion. The obtained experimental data testify to the high therapeutic potential of the leukocyte protein-peptide complex, which has a wide spectrum of antibacterial activity.
About the Authors
A. G. Volkov
Perm State Medical University named after Academician E. A. Wagner
Russian Federation
Russian Federation
Alexander G. Volkov — Ph. D. in Medicine, senior lecturer at the Department of Pharmacology
Perm
L. I. Kononova
Institute of Ecology and Genetics of Microorganisms of the Ural Branch of the Russian Academy of Sciences
Russian Federation
Russian Federation
Lyudmila I. Kononova — leading engineer of the Laboratory of Biochemistry of Microorganism Development
Perm
V. P. Korobov
Institute of Ecology and Genetics of Microorganisms of the Ural Branch of the Russian Academy of Sciences; Perm National Research Polytechnic University
Russian Federation
Russian Federation
Vladimir P. Korobov — Ph. D. in Medicine, Associate Professor of the Department of Chemistry and Biotechnology; Head of the Laboratory of Biochemistry of Microorganism Development
Perm
L. V. Volkova
Perm National Research Polytechnic University
Russian Federation
Russian Federation
Larisa V. Volkova — D. Sc. in Medicine, Professor of the Department of Chemistry and Biotechnology
Perm
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Review
For citations:
Volkov A.G., Kononova L.I., Korobov V.P., Volkova L.V. Study of Molecular Parameters and Antibacterial Activity of a Peptide Preparation Derived from Human Leukocytes. Antibiot Khimioter = Antibiotics and Chemotherapy. 2023;68(9-10):20-24. (In Russ.) https://doi.org/10.37489/0235-2990-2023-68-9-10-20-24
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