Non-tuberculous Mycobacteria: Basic Properties and Methods of Their Cultivation
https://doi.org/10.37489/0235-2990-2025-70-5-6-58-63
EDN: CGGRHI
Abstract
In recent years, an increase in the incidence of mycobacteriosis was observed. Diagnosis of mycobacteriosis remains extremely difficult, primarily due to the similarity of the clinical and radiological picture with that of tuberculosis. Cultivation of mycobacteria requires an individual approach depending on the type of mycobacteria and the conditions of their cultivation. Coverage and implementation of cultivation methods contribute to increasing the efficiency of diagnosis and treatment of diseases caused by mycobacteria. The prevalence of mycobacteriosis, which today represents a serious problem for both clinicians and laboratory specialists, requires the development of a new strategy for their microbiological diagnostics, allowing for rapid detection of drug resistance, identification of non-tuberculous mycobacteria, and the prescription of an adequate chemotherapy regimen, as well as increasing the effectiveness of treatment and preventing the spread of drug-resistant strains of mycobacteria.
Supporting agencies: The work was carried out as part of the state assignment of the Ministry of Health of the Russian Federation in terms of conducting research and development on the topic "Development of test systems for screening PCR diagnostics, monitoring the course of mycobacteriosis, and species PCR identification of mycobacteria"
About the Authors
G. N. Genatullina
Astrakhan State Medical University of the Ministry of Health of Russian Federation
Russian Federation
Russian Federation
Guzel N. Genatullina, Ph. D. in Biology, Associate Professor, Deputy Head of the Center, Associate Professor of the Department
Research Center; Department of Pharmacognosy, Pharmaceutical Technology, and Biotechnology
Astrakhan
A. L. Yasenyavskaya
Astrakhan State Medical University of the Ministry of Health of Russian Federation
Russian Federation
Russian Federation
Anna L. Yasenyavskaya, Ph. D. in Medicine, Associate Professor, Head of the Center, Professor
Research Center; Department of Pharmacognosy, Pharmaceutical Technology, and Biotechnology
Astrakhan
M. A. Samotrueva
Astrakhan State Medical University of the Ministry of Health of Russian Federation
Russian Federation
Russian Federation
Marina A. Samotrueva, D. Sc. in Medicine, Professor, Head of the Department, Chief Researcher
Department of Pharmacognosy, Pharmaceutical Technology, and Biotechnology; Research Center
Astrakhan
O. V. Rubalsky
Astrakhan State Medical University of the Ministry of Health of Russian Federation
Russian Federation
Russian Federation
Oleg V. Rubalsky, D. Sc. in Medicine, Professor, Head of the Department, Chief Researcher
Department of Microbiology and Virology; Research Center
Astrakhan
References
1. Petrova F. S., Petrov I. V., Amirova T. H., Petrova L. V. Mikobakterioz: obzor dokazannyh klinicheskih proyavlenij u cheloveka. Vestnik Avicenny. 2020; 22 (3): 484–490. doi: 10.25005/2074-0581-2020-22-3-484-490 (in Russian)
2. Daley C. L., Griffith D. E. Pulmonary non-tuberculous mycobacterial infections. Int. J. Tuberc. Lung. Dis. 2010; 14 (6): 665–671.
3. Gerasimova E. N., Ismatullin D. D., Lyamin A. V., Zhestkov A. V. Obshchaya harakteristika, osobennosti kul'tivirovaniya i antibiotikorezistentnosti predstavitelej Mycobacterium fortuitum group (obzor literatury). Klinicheskaya Laboratornaya Diagnostika. 2021; 66 (4): 223–228. doi: 10.51620/0869-2084-2021-66-4-223-228 (in Russian)
4. Lyamin A. V., Zhestkov A. V., Ismatullin D. D., Kovalev A. M. Laboratornaya diagnostika mikobakteriozov. Vestnik Sovremennoj Klinicheskoj mediciny. 2017; 10 (1): 29–35. doi: 10.20969/VSKM.2017.10 (1).29-35 (in Russian)
5. Nogueira L. B., Garcia C. N., Costa M. S. C., Moraes M. B., Kurizky P. Sh., Gomes C. M. Non-tuberculous cutaneous mycobacterioses. An. Bras. Dermatol. 2021; 96 (05): 527–538. doi: 10.1016/j.abd.2021.04.005
6. Sprenger M., Fukuda K. New mechanisms, new worries. Science. 2016; 351: 1263–1264. doi: 10.1126/science.aad9450
7. Lyamin A. V., Ismatullin D. D., Zhestkov A. V., Kondratenko O. V. Laboratornaya diagnostika mikobakteriozov u pacientov s mukoviscidozom (obzor literatury). Klinicheskaya Laboratornaya Diagnostika. 2018; 63 (5): 315–320. doi: 10.18821/0869-2084-2018-63-5-315-320 (in Russian)
8. Petrov I. V., Amirova T. H., Petrova L. V., Petrova F. S. Problemy epidemiologii i diagnostiki mikobakterioza sredi immunokomprometirovannyh pacientov (na primere VICh-infekcii). Vestnik CNIIT. 2019; 1: 43–44 (in Russian)
9. Makarova M. V., Guntupova L. D. Netuberkuleznye mikobakterii. BIOpreparaty. Profilaktika, Diagnostika, Lechenie. 2020; 20 (2): 97–102. doi: 10.30895/2221-996X-2020-20-2-97-102 (in Russian)
10. Ergeshov A. E., Shmelev E. I., Kovalevskaya M. N. i dr. Netuberkuleznye mikobakterii u pacientov s zabolevaniyami organov dyhaniya (kliniko-laboratornoe issledovanie). Pul'monologiya. 2016; 26 (3): 303–308. (in Russian)
11. Dolgova V. V., Men'shikova V. V. Klinicheskaya laboratornaya diagnostika : nacional'noe rukovodstvo : v 2 t. M.: GEOTAR-Media, 2013. T. II., 808 s (in Russian)
12. Tortoli E., Rindi L., Garcia M. J. et al. Proposal to elevate the genetic variant MAC-A, included in the Mycobacterium avium complex, to species rank as Mycobacterium chimaera sp. nov. Int. J. Syst. Evol. Micr. 2004; 54: 1277–1285.
13. Adekambi Т., Berger P., Raoult D., Drancourt M. RpoB gene sequence-based characterization of emerging non-tuberculous mycobacteria with descriptions of Mycobacterium bolletii sp. nov., Mycobacterium phocaicum sp. nov. and Mycobacterium aubagnense sp. nov. Int. J. Syst. Evol. Micr. 2006; 56: 133–143.
14. Petrov I. V., Novikova M. O., Petrova L. V. et al. Prevention of healthcare associated infections in the tuberculosis health center. Helix Int. J. 2017; 8 (1): 2958–2963.
15. Burova P. O. Sovremennye metody diagnostiki mikobakteriozov. FORCIPE. 2019; prilozhenie, 595–596 (in Russian)
16. Golyshevskaya V. I., Sevast'yanova E. V. Sovremennoe sostoyanie mikrobiologicheskoj diagnostiki tuberkuleza v Rossii. Aktual'nye problemy tuberkuleza i boleznej legkih : materialy nauchnoj sessii, posvyashchennoj 85-letiyu CNIIT RAMN. 2006; 17–18. (in Russian)
17. Lopakov K. V., Sabgajda T. P., Popov S. A. Novyj integral'nyj pokazatel' «epidemiologicheskij potencial tuberkuleza». Social'nye aspekty zdorov'ya naseleniya. 2009; 1, 4 (in Russian)
18. Golyshevskaya V. I., Sevast'yanova E. V., Irtuganova O. A., Erohin V. V. Sovremennoe sostoyanie laboratornoj sluzhby Rossii po diagnostike tuberkuleza: osnovnye problemy i puti ih preodoleniya. Problemy tuberkuleza i boleznej legkih. 2006; 12: 36–42 (in Russian)
19. Springer B., Stockman L., Tescher K. Two-laboratory collaborative study on identification of mycobacteria: molecular versus phenotypic method. J. Clin. Microbiol. 1996; 34: 296–303.
Review
For citations:
Genatullina GN, Yasenyavskaya AL, Samotrueva MA, Rubalsky OV. Non-tuberculous Mycobacteria: Basic Properties and Methods of Their Cultivation. Antibiotiki i Khimioterapiya = Antibiotics and Chemotherapy. 2025;70(5-6):58-63. (In Russ.) https://doi.org/10.37489/0235-2990-2025-70-5-6-58-63. EDN: CGGRHI
Views:
298
JATS XML
Email this article 