In Vitro Study of Antimicrobial Activity of Lactoferrins from Various Sources
Abstract
Comparative antimicrobial activity of lactoferrins from various sources (native lactoferrin from Laprot, human hololactoferrin, recombinant human lactoferrin isolated from the cultural medium of permissive cell culture transfected using pseudoadenovirus nanostructure with the human lactoferrin gene, and native bovine lactoferrin) was studied to prove the possibility of their use for development of antimicrobial drugs. It was shown that all the substances were active against the Bacillus standard strains. The antibacterial activity was almost independent of the degree of saturation the lactoferrin molecules with Fe3+. The native human lactoferrin was more active than hololactoferrin against Candida when evaluated by the minimum inhibitory concentration (MIC). Fe3+-Non aturated recombinant human lactoferrin demonstrated the antimicrobial activity (by MIC) similar to that of the native human lactoferrin. The results showed that native and recombinant human lactoferrins might be used for the development of intravenous and intracavitary dosage forms, while the native bovine lactoferrin could be useful in development of oral drugs.
About the Authors
N. E. Grammatikova
I. M. Sechenov Moscow Medical Academy
Russian Federation
Russian Federation
S. P. Rezvan
I. M. Sechenov Moscow Medical Academy
Russian Federation
Russian Federation
E. R. Nemtsova
P. A. Gerzen Moscow Research Oncologic Institute
Russian Federation
Russian Federation
O. A. Bezborodova
P. A. Gerzen Moscow Research Oncologic Institute
Russian Federation
Russian Federation
I. L. Tutykhina
N. F. Gamaleya Research Institute of Epidemiology and Microbiology, Academy of Medical Sciences
Russian Federation
Russian Federation
B. S. Naroditsky
N. F. Gamaleya Research Institute of Epidemiology and Microbiology, Academy of Medical Sciences
Russian Federation
Russian Federation
R. I. Yakubovsky
P. A. Gerzen Moscow Research Oncologic Institute
Russian Federation
Russian Federation
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Review
For citations:
Grammatikova N.E., Rezvan S.P., Nemtsova E.R., Bezborodova O.A., Tutykhina I.L., Naroditsky B.S., Yakubovsky R.I. In Vitro Study of Antimicrobial Activity of Lactoferrins from Various Sources. Antibiot Khimioter = Antibiotics and Chemotherapy. 2010;55(7-8):4. (In Russ.)
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