Investigation of Ampicillin Effect on Morphological and Mechanical Properties of Escherichia coli and Bacillus cereus Cells with Atomic Force Microscopy
Abstract
The effect of subbacteriostatic concentrations of ampicillin on morphological and mechanical properties of gramnegative and grampositive cells of Escherichia coli K12 TG1 and Bacillus cereus IP 5832 respectively was studied with atomic force microscopy. Significant heterogeneity of the bacterial populations was shown by the character of the response to the antibiotic effect. The common feature was increase of the cell size likely due to the effect of the inner osmotic pressure on the lowered cell wall strength. In the E.coli population there were besides observed anomalous elongated cells with signs of septation disorder, as well as their structurs, lacking the cytoplasmic liquid fraction. In the B.cereus the inner osmotic pressure mainly enlarged the cell cross section, changing the cell shape from rod to sphere, that was accompanied by significant impairment of the surface structure with liberation of the peptidoglycane fragments to the medium. The particular features of the E.coli K12 TG1 and B.cereus IP 5832 respond to the ampicillin effect were attributed to the differences in the structure of their cell wall, also due to specific properties of the peptidoglycane synthesis and three-dimensional organization.
About the Authors
D. G. Deryabin
Оренбургский государственный университет
Russian Federation
Russian Federation
A. S. Vasilchenko
Оренбургский государственный университет
Russian Federation
Russian Federation
A. N. Nikiyan
Оренбургский государственный университет
Russian Federation
Russian Federation
References
1. Finberg R.V., Moellering R.C., Tally F.P. The Importance of bactericidal drugs: future directions in infectious disease. Clin Infect Dis 2004; 39: 1314-1320.
2. Binnig G., Quate C.F., Gerber Ch. Atomic force microscope. Phys Rev Lett 1986; 6: 59: 930-933.
3. Dufrêne Y.F. Atomic force microscopy, a powerful tool in microbiology. J Bacteriol 2002; 184: 19: 5205-5213.
4. Camesano T.A., Natan M.J., Logan B.E. Observation of changes in bacterial cell morphology using tapping mode atomic force microscopy. Langmuir 2000; 16: 4563-4572.
5. Олюнина Л.H., Мацкова Ю.A., Гончарова Т.A., Гущина Ю.Ю. Оценка терморезистентности Azotobacter chrococcum методом атомно-силовой микроскопии. Приклад. биохим. микробиол. 2009; 45: 1: 45-50.
6. Perry C.C., Weatherly M., Beale T., Randriamahefa A. Atomic force microscopy study of the antimicrobial activity of aqueous garlic versus ampicillin against Escherichia coli and Staphylococcus aureus. J Sci Food Agric 2009; 89: 958-964.
7. Yang L., Wang K., Tan W. et al. Atomic force microscopy study of different effects of natural and semisynthetic β-lactam on the cell envelope of Escherichia coli. Anal Chem 2006; 78: 7341-7345.
8. Рачина C.A., Козлов Р.С., Шаль E.П. и др. Анализ антибактериальной терапии госпитализированных пациентов с внебольничной пневмонией в различных регионах: уроки многоцентрового фармакоэпидемиологического исследования. Клин. микробиол. антимикроб. химиотер. 2009; 11: 1: 66-78.
9. Nikiyan A., Vasilchenko A., Deryabin D. Humidity-dependent bacterial cells functional morphometry investigations using atomic force microscope. Intern J Microbiol 2010; Article ID 704170, doi:10.1155/2010/704170.
10. Голутвин И.A., Насикан И.С., Игнатюк T.E. Новые подходы к исследованию вирусов при помощи сканирующей зондовой микроскопии. Биофизика. 2004; 49: 6: 1105-1111.
11. Salerno M., Bykov I. Tutorial: mapping adhesion forces and calculating elasticity in contact-mode AFM. Microscopy and Analysis 2006; 20: S5-S8.
12. Spratt B.G. Distinct penicillin binding proteins involved in the division, elongation, and shape of Escherichia coli K12. Proc Nat Acad Sci USA 1975; 72: 8: 2999-3003.
Review
For citations:
Deryabin D.G., Vasilchenko A.S., Nikiyan A.N. Investigation of Ampicillin Effect on Morphological and Mechanical Properties of Escherichia coli and Bacillus cereus Cells with Atomic Force Microscopy. Antibiot Khimioter = Antibiotics and Chemotherapy. 2011;56(7-8):7-12. (In Russ.)
Views:
343
Email this article 