Study on Phenotypical Activity of Isoenzyme CYP3A4 in Children
Abstract
The urine levels of cortisol and 6-β-hydroxycortisol in 30 healthy children were determined with high performance liquid chromatography. The activity of cytochrome P450 isoenzyme CYP3A4 was estimated by the ratio of 6-β-hydroxycortisol and cortisol. Differences in the CYP3A4 activity depended on the age sex. At the age of 4 to 9 years the value of the ratio was 9.21+0.67 which in fact was statistically higher than that in the children at the age of 0 to 3 years (p<0.001). In the female children at the age of 0 to 3 years the value of the isoenzyme CYP3A4 activity was actually lower (p<0,05) vs. the female children of the higher ages and the male children at the age of 0 to 3 years. The results are useful for further researches on improvement of drugs dosing and prevention of adverse reactions.
About the Authors
B. I. Kantemirova
Astrakhan State Medical Academy
Russian Federation
Russian Federation
A. KH. Chernysheva
Astrakhan State Medical Academy
Russian Federation
Russian Federation
A. K. Starodubtsev
I. M. Sechenov 1st Moscow State Medical University
Russian Federation
Russian Federation
D. A. Sychev
I. M. Sechenov 1st Moscow State Medical University
Russian Federation
Russian Federation
References
1. Баранов В.С., Баранова Е.В., Иващенко Т.Э., Асеев М.В. Геном человека и гены «предрасположенности». СПб.: 2000; 272.
2. Кукес В.Г. Метаболизм лекарственных средств: клинико-фармакологические аспекты. М.: 2004; 144.
3. Кукес В.Г., Сычев Д.А., Раменская Г.В., Игнатьев И.В. Фармакогенетика системы биотрансформациии транспортеров лекарственных средств: от теории к практике. Биомедицина 2007; 6: 29-46.
4. Щепотина Е.Г. Полиморфизм генов цитохрома Р450 подсемейства 3А и вариабельная активность CYP3A4: автореф. дис.. канд. биол. наук. Новосибирск, 2008; 24.
5. Steinbrook R. Testing medications in children. N Eng J Med. 2002; 347: 18.
6. William E.E., Pharm D., Howard L.M. Pharmacogenomics - drug disposition, drug targets, and side effects. N Engl J Med 2003; 348: 538-549.
7. Кукес В.Г., Грачев С.В., Сычев Д.А. Метаболизм лекарственных средств. Науч. основ. персонализир. мед. 2008; 304.
8. Зборовский А.Б., Тюренков И.Н. Осложнения фармакотерапии. Руководство для врачей. М.: 2003; 544.
9. Смирнов В.В., Савченко А.Ю., Раменская Г.В. Разработка методики количественного определения эндогенного кортизола и 6-β-гидроксикортизола в моче с целью определения активности изофермента CYP 3A4. Биомедицина 2010; 4: 56-60.
10. Johnson T.N., Rostami-Hodjegan A., Tucker G.T. Prediction of the clearance of eleven drugs and associated variability in neonates, infants and children. Clin Pharmacokinet 2006; 45: 9: 931-956.
11. Johnson T.N., Rostami-Hodjegan A., Tucker G.T. Development of CYP2D6 and CYP3A4 in the first year of life. Clin Pharmacol Ther 2007; 81: 4: 510-516.
Review
For citations:
Kantemirova B.I., Chernysheva A.Kh., Starodubtsev A.K., Sychev D.A. Study on Phenotypical Activity of Isoenzyme CYP3A4 in Children. Antibiot Khimioter = Antibiotics and Chemotherapy. 2012;57(1-2):23-25. (In Russ.)
Views:
335
Email this article 