Analysis of Amoxicillin’s Antibacterial Activity Using Biological Sensor With Clit Acoustic Wave
https://doi.org/10.37489/0235-2990-2021-66-1-2-12-18
Abstract
The possibility of antibacterial activity assay using an acoustic non-contact biological sensor based on two piezoelectric
plates separated by an air gap was demonstrated on the example of amoxicillin and Escherichia coli for the first time. An acoustic wave with transverse horizontal polarization is excited in the bottom plate of the sensor. The upper plate serves as the bottom of the container with the studied cell suspension. It was shown that the addition of an antibiotic to the cell suspension leads to a change in the parameters of the sensor. The effect of amoxicillin on microbial cells was monitored by laser microscopy and standard microbiological culture. The possibility of express analysis of the drug’s antibacterial activity using a biological sensor based on the use of a slit acoustic wave is shown.
About the Authors
О. I. Guliy
Institute of Biochemistry and Physiology of Plants and Microorganisms of the Russian Academy of Sciences; Saratov State Vavilov Agrarian University
Russian Federation
Russian Federation
Olga I. Guliy — D.Sc. in biology, Professor
Saratov
В. D. Zaitsev
Kotelnikov Institute of Radio Engineering and Electronics of the Russian Academy of Sciences
Russian Federation
Russian Federation
Boris D. Zaitsev — D.Sc. in physics and mathematics, Professor
Saratov
О. S. Larionova
Saratov State Vavilov Agrarian University
Russian Federation
Russian Federation
Olga S. Larionova — D.Sc. in biology
Saratov
A. M. Alsowaidi
Institute of Biochemistry and Physiology of Plants and Microorganisms of the Russian Academy of Sciences
Russian Federation
Russian Federation
AliKadhim Mohammed Alsowaidi — full-time postgraduate student
О. A. Karavaeva
Institute of Biochemistry and Physiology of Plants and Microorganisms of the Russian Academy of Sciences
Russian Federation
Russian Federation
Olga A. Karavaeva — Ph.D. in biology
А. М. Peterson
Saratov State University
Russian Federation
Russian Federation
Alexandra M. Peterson — Ph.D. in biology
I. A. Borodina
Kotelnikov Institute of Radio Engineering and Electronics of the Russian Academy of Sciences
Russian Federation
Russian Federation
Irina A. Borodina — Ph.D. in physics and mathematics
References
1. Antibiotic Resistance Protocols: Second Edition, Gillespie SH, McHugh TD (eds.), Methods in Molecular Biology. Vol. 642. Springer Science+Business Media. LLC 2010.
2. Balouiri M., Sadiki M., Ibnsouda S.K. Methods for in vitro evaluating antimicrobial activity: A review. J Pharm Anal. 2016; 6 (2): 71–79. doi: 10.1016/j.jpha.2015.11.005
3. Matuschek E., Brown D. F. J., Kahlmeter G. Development of the EUCAST disk diffusion antimicrobial susceptibility testing method and its implementation in routine microbiology laboratories. Clin Microbiol Infect. 2014; 20: O255–O266. doi: 10.1111/1469-0691.12373
4. Gulij O.I., Zajtsev B.D., Semenov A.S., Larionova O.S., Karavaeva O.A., Borodina I.A. Otsenka vozdejstviya amoksitsillina na mikrobnye kletki metodom elektroakusticheskogo analiza. Biofizika. 2018. 63 (3): 496–502. doi: 10.1134/S0006350918030089 (in Russian)
5. Gulij O.I., Zajtsev B.D., Karavaeva O.A., Lovtsova L.G., Mekhta S.K., Borodina I.A. Ekspress-analiz chuvstvitel'nosti bakterij k beta-laktamnym antibiotikam antibiotikam s pomoshch'jyu rezonatora s poperechnym elektricheskim polem. Antibiot khimioter. 2019; 64 (1–2): 3–8. doi: 10.24411/0235W2990W2019W10001 (in Russian)
6. Borodina I.A., Zaitsev B.D., Teplykh A.A. The influence of viscous and conducting liquid on characteristics of slot acoustic wave. Ultrasonics. 2018; 82: 39–43. doi: 10.1016/j.ultras.2017.07.011
7. European Medicines Agency, European Surveillance of Veterinary Antimicrobial Consumption, 2017. ‘Sales of veterinary antimicrobial agents in 30 European countries in 2015’. (EMA/184855/2017).
8. Borodina I.A., Zaitsev B.D., Kuznetsova I.E., Teplykh A.A. Acoustic Waves in a Structure Containing Two Piezoelectric Plates Separated by an Air (Vacuum) Gap. IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control. 2013; 60 (12): 2677–2281. doi: 10.1109/TUFFC.2013.2867
9. Metody obshchej bakteriologii pod redaktsiej F.Gerk harda. Moscow: Mir, 1983; 1: 458–464 (in Russian)
10. Johnson W.L., France D.C., Rentz N.S., Cordell W.T., Walls F.L. Sensing bacterial vibrations and early response to antibiotics with phase noise of a resonant crystal. Scientific Reports. 2017; 7: 12138. doi: 10.1038 / s41598 -017-12063-6
11. Yao Z., Kahne D., Kishoy R. Distinct single-cell morphological dynamics under beta-lactam antibiotics.
Review
For citations:
Guliy О.I., Zaitsev В.D., Larionova О.S., Alsowaidi A.M., Karavaeva О.A., Peterson А.М., Borodina I.A. Analysis of Amoxicillin’s Antibacterial Activity Using Biological Sensor With Clit Acoustic Wave. Antibiot Khimioter = Antibiotics and Chemotherapy. 2021;66(1-2):12-18. (In Russ.) https://doi.org/10.37489/0235-2990-2021-66-1-2-12-18
Views:
643
Email this article 