Immunoadjuvant activity of marine bacteria exopolysaccharides in normal and immunosuppressive conditions
T. A. Kuznetsova,
E. V. Persiyanova,
L. A. Ivanushko,
T. P. Smolina,
A. K. Gazha,
M. S. Kokulin,
N. N. Besednova https://doi.org/10.37489/0235-2990-2021-66-5-6-17-22
Abstract
Aim. To assess the mechanisms of immunoadjuvant activity of marine bacteria exopolysaccharides (PS) in normal and immunosuppressive conditions in vivo. Material and methods. Experimental studies were carried out on male ВALB/c mice, immunized with compositions of PS or aluminum hydroxide and ovalbumin (OVA). Immunosuppression was induced by triple intraperitoneal administration of dexamethasone (2 mg/kg). Serum levels of specific antibodies (IgG, IgG1, and IgG2a) and cytokines (IFN-γ, IL-2, IL-10) were determined. Results. The inclusion of PS to the immune compositions led to the formation of an enhanced specific IgG, IgG1, and IgG2a to OVA, comparable to the effect of aluminum hydroxide. The studied PS also contributed to an increase in the level of both pro- and anti-inflammatory cytokines. Conclusion. PS from marine bacteria act as adjuvants in normal and immunosuppressive conditions, stimulating a mixed Th1 (IgG2a, INF-γ) and Th2 (IgG1, IL-10) immune response to OVA.
About the Authors
T. A. Kuznetsova
G. P. Somov Research Institute of Epidemiology and Microbiology
Russian Federation
Russian Federation
Tatyana A. Kuznetsova — D. Sc. in medicine
ResearcherID: I-8399-2018; eLIBRARY SPIN code: 2359-1132; Scopus Author ID: 7202571979
1 Selskaya, G. P. Somov Research Institute of Epidemiology and Microbiology, Vladivostok
E. V. Persiyanova
G. P. Somov Research Institute of Epidemiology and Microbiology
Russian Federation
Russian Federation
Elena V. Persiyanova — Ph. D. in biology
ResearcherID: I-3513-2018; eLIBRARY SPIN: 8385-7621; Scopus Author ID: 56311963200
Vladivostok
L. A. Ivanushko
G. P. Somov Research Institute of Epidemiology and Microbiology
Russian Federation
Russian Federation
Lyudmila A. Ivanushko — Ph. D. in medicine
ResearcherID: K-2879-2018; eLIBRARY SPIN: 1535-7665; Scopus Author ID: 6507058048
Vladivostok
T. P. Smolina
G. P. Somov Research Institute of Epidemiology and Microbiology
Russian Federation
Russian Federation
Tatyana P. Smolina — Ph. D. in biology
ResearcherID: J-4344-2018; eLIBRARY SPIN code: 3503-8898; Scopus Author ID: 6603763859
Vladivostok
A. K. Gazha
G. P. Somov Research Institute of Epidemiology and Microbiology
Russian Federation
Russian Federation
Anna K. Gazha — Ph. D. in medicine
ResearcherID: J-4350-2018; eLIBRARY SPIN code: 1960-4471; Scopus Author ID: 6508049418
Vladivostok
M. S. Kokulin
G. B. Elyakov Pacific Institute of Bioorganic Chemistry
Russian Federation
Russian Federation
Maksim S. Kokulin — Ph. D. in chemistry
ResearcherID: N-8353-2013; eLIBRARY SPIN: 9170-5830; Scopus Author ID: 54410589100
Vladivostok
N. N. Besednova
G. P. Somov Research Institute of Epidemiology and Microbiology
Russian Federation
Russian Federation
Natalia N. Besednova — D.Sc. in medicine, Professor, Academician of the Russian Academy of Sciences, G. P. Somov Research Institute of Epidemiology and Microbiology
eLIBRARY SPIN: 8931-9002; Scopus Author ID: 7006805123
Vladivostok
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Review
For citations:
Kuznetsova T.A., Persiyanova E.V., Ivanushko L.A., Smolina T.P., Gazha A.K., Kokulin M.S., Besednova N.N. Immunoadjuvant activity of marine bacteria exopolysaccharides in normal and immunosuppressive conditions. Antibiot Khimioter = Antibiotics and Chemotherapy. 2021;66(5-6):17-22. (In Russ.) https://doi.org/10.37489/0235-2990-2021-66-5-6-17-22
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