Antibiotic Sensitivity of Neisseria meningitidis Isolated From Patients with Generalized Forms of Meningococcal Infection and From Healthy Carriers
https://doi.org/10.37489/0235-2990-2022-67-5-6-19-24
Abstract
The study included 50 isolates of Neisseria meningitidis isolated from patients with generalized forms of meningococcal infection and 48 isolates, which were isolated from carriers. The isolates were identified by MALDI-TOF mass spectrometry using a Microflex LT mass spectrometer (Bruker Daltonics, Germany). Minimum inhibitory concentrations (MICs) of benzylpenicillin, ampicillin, ceftriaxone, meropenem, ciprofloxacin, azithromycin, rifampicin, and chloramphenicol were assessed by the method of serial agar dilution with interpretation of the results according to EUCAST 2021 criteria. Clinical resistance to penicillin was detected in 7% of isolates. However, all isolates with MIC >0.064 µg/ml (n=26) had mutations in the penA gene. Decreased sensitivity to rifampicin was found in four isolates isolated from patients and four isolated from carriers. Seven out of eight isolates with reduced sensitivity to rifampicin had mutations in the rpoB gene. Resistance to ceftriaxone, meropenem, ciprofloxacin was not detected.
About the Authors
E. A. MARTENS
Pediatric Research and Clinical Center for Infectious Diseases under the Federal Medical Biological Agency; North-Western State Medical University named after I. I. Mechnikov
Russian Federation
Russian Federation
Elvira A. Martens — Head of the Clinical Diagnostic Laboratory, Junior Researcher, Pediatric Research and Clinical Center for Infectious Diseases under the Federal Medical Biological Agency; Assistant at the Department of Medical Microbiology North-Western State Medical University named after I. I. Mechnikov.
WOS Researcher ID: CAG4447-2022
Scopus Author ID: 57206470215
Saint-Petersburg
L. I. ZHELEZOVA
Pediatric Research and Clinical Center for Infectious Diseases under the Federal Medical Biological Agency
Russian Federation
Russian Federation
Lyudmila I. Zhelezova — Ph. D. in medicine.
WOS Researcher ID: G-9662-2012
Scopus Author ID: 6505716185
Saint-Petersburg
V. V. GOSTEV
Pediatric Research and Clinical Center for Infectious Diseases under the Federal Medical Biological Agency; North-Western State Medical University named after I. I. Mechnikov
Russian Federation
Russian Federation
Vladimir V. Gostev — Ph. D. in biology, Pediatric Research and Clinical Center for Infectious Diseases under the Federal Medical Biological Agency, North-Western State Medical University named after I. I. Mechnikov.
WOS Researcher ID: P-1949-2016
WOS Researcher ID: 55614534400
197022, St. Petersburg, Professora Popova st., 9
D. V. LIKHOLETOVA
Pediatric Research and Clinical Center for Infectious Diseases under the Federal Medical Biological Agency
Russian Federation
Russian Federation
Daria V. Likholetova — Laboratory researcher at the Department of Medical Microbiology and Molecular Epidemiology.
Saint-Petersburg
D. P. GLADIN
Saint-Petersburg State Pediatric Medical University
Russian Federation
Russian Federation
Dmitry P. Gladin — Ph. D. in medicine.
Scopus Author ID: 6603374770
Saint-Petersburg
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For citations:
MARTENS E.A., ZHELEZOVA L.I., GOSTEV V.V., LIKHOLETOVA D.V., GLADIN D.P. Antibiotic Sensitivity of Neisseria meningitidis Isolated From Patients with Generalized Forms of Meningococcal Infection and From Healthy Carriers. Antibiot Khimioter = Antibiotics and Chemotherapy. 2022;67(5-6):19-24. (In Russ.) https://doi.org/10.37489/0235-2990-2022-67-5-6-19-24
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