Study on Antiviral Activity and Pharmacological Safety of Double-Stranded Ribonucleic Acid for Intranasal Administration
S. G. Gamaley,
G. G. Shimina,
E. S. Tsyplenkova,
O. V. Simakova,
M. O. Skarnovich,
M. A. Skarnovich,
L. N. Shishkina,
O. S. Taranov,
O. S. Ivanova,
G. M. Levagina,
E. D. Danilenko https://doi.org/10.37489/0235-2990-2022-67-9-10-42-48
Abstract
Interferon inducers are of interest as the first line of defense against viral infections, including influenza. Double-stranded ribonucleic acid (dsRNA) for intranasal administration has been developed, the excipients and drug components were selected at the Institute of Medical Biotechnology, a branch of the State Research Center of Virology and Biotechnology «Vector». This work presents the results of the study on antiviral activity and pharmacological safety of a new form of dsRNA. The antiviral activity of the preparation was studied in BALB/c mice infected with influenza A/Aichi/2/68 (H3N2) or Bishkek/01/2009 (H1N1pdm09) viruses. A safety study was performed with a single administration of intranasal dsRNA into white outbred ICR mice. The study showed that the administration of therapeutic and prophylactic regimen of intranasal dsRNA at a dose of 2.5 mg/kg increases the survival and average life expectancy of mice infected with the mentioned strains of influenza virus. The protective effect of the preparation in mice infected with a lethal dose of Bishkek/01/2009 virus was comparable to the effect of Tamiflu. The absence of toxic effects of intranasal dsRNA at a pharmacological dose in laboratory mice, the functional state of their physiological systems, as well as main types of metabolism, established in the experiments, lead to a conclusion concerning pharmacological safety of the preparation and the prospects for further work to complete pharmaceutical development of a new antiviral drug.
About the Authors
S. G. Gamaley
State Research Center of Virology and Biotechnology «Vector» of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing
Russian Federation
Russian Federation
Svetlana G. Gamaley — Head of the Department of Biological Studies
ResearcherID: B-7418-2014
eLIBRARY SPIN-код: 6593-3420
Scopus Author ID: 6504003751
Koltsovo, Novosibirsk Region
G. G. Shimina
State Research Center of Virology and Biotechnology «Vector» of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing
Russian Federation
Russian Federation
Galina G. Shimina — Researcher at the Department of Biological Studies
Koltsovo, Novosibirsk Region
E. S. Tsyplenkova
State Research Center of Virology and Biotechnology «Vector» of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing
Russian Federation
Russian Federation
Elena S. Tsyplenkova — Junior researcher at the Department of Biological Studies
Koltsovo, Novosibirsk Region
O. V. Simakova
State Research Center of Virology and Biotechnology «Vector» of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing
Russian Federation
Russian Federation
Olga V. Simakova — Junior researcher at the Department of Biological Studies
Koltsovo, Novosibirsk Region
M. O. Skarnovich
State Research Center of Virology and Biotechnology «Vector» of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing
Russian Federation
Russian Federation
Maxim O. Skarnovich — M. D., Senior researcher at the Department of Extremely Hazardous Virus Infections Prevention and Treatment
eLIBRARY SPIN-код: 8405-5175
Scopus Author ID: 57189059427
Koltsovo, Novosibirsk Region
M. A. Skarnovich
State Research Center of Virology and Biotechnology «Vector» of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing
Russian Federation
Russian Federation
Maria A. Skarnovich — Researcher at the Department of Extremely Hazardous Virus Infections Prevention and Treatment
eLIBRARY SPIN-код: 6381-5817
Scopus Author ID: 57189059427
Koltsovo, Novosibirsk Region
L. N. Shishkina
State Research Center of Virology and Biotechnology «Vector» of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing
Russian Federation
Russian Federation
Larisa N. Shishkina — D. Sc. in Biology, Head of the Department of Extremely Hazardous Virus Infections Prevention and Treatment
ResearcherID: B-2263-2014
eLIBRARY SPIN-код: 7165-4367
Scopus Author ID: 35316454500
Koltsovo, Novosibirsk Region
O. S. Taranov
State Research Center of Virology and Biotechnology «Vector» of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing
Russian Federation
Russian Federation
Oleg S. Taranov — Head of the Department of Microscopy
ResearcherID: A-5657-2014
eLIBRARY SPIN-код: 5894-6518
Scopus Author ID: 8938697800
Koltsovo, Novosibirsk Region
O. S. Ivanova
State Research Center of Virology and Biotechnology «Vector» of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing
Russian Federation
Russian Federation
Olga S. Ivanova — Ph. D. in Biology, Researcher at the Department of Technology Development and Pilot Production of Biologicals
eLIBRARY SPIN-код: 4165-6310
Koltsovo, Novosibirsk Region
G. M. Levagina
State Research Center of Virology and Biotechnology «Vector» of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing
Russian Federation
Russian Federation
Galina M. Levagina — Ph. D. in Biology, Head of the Department of Technology Development and Pilot Production of Biologicals
ResearcherID: C-7590-2014
eLIBRARY SPIN-код: 6837-4940
Scopus Author ID: 6506245503
Koltsovo, Novosibirsk Region
E. D. Danilenko
State Research Center of Virology and Biotechnology «Vector» of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing
Russian Federation
Russian Federation
Elena D. Danilenko — Ph. D. in Biology, Director of the Institute of Medical Biotechnology
ResearcherID: A-7083-2014
eLIBRARY SPINкод: 1388-4127
Scopus Author ID: 7004245682
Koltsovo, Novosibirsk Region
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For citations:
Gamaley S.G., Shimina G.G., Tsyplenkova E.S., Simakova O.V., Skarnovich M.O., Skarnovich M.A., Shishkina L.N., Taranov O.S., Ivanova O.S., Levagina G.M., Danilenko E.D. Study on Antiviral Activity and Pharmacological Safety of Double-Stranded Ribonucleic Acid for Intranasal Administration. Antibiot Khimioter = Antibiotics and Chemotherapy. 2022;67(9-10):42-48. (In Russ.) https://doi.org/10.37489/0235-2990-2022-67-9-10-42-48
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