Sensitivity to antibacterial drugs of biofilms formed by strains of vibrio cholerae of various serogroups.
https://doi.org/10.37489/0235-2990-2023-68-1-2-16-21
Abstract
The ability of Vibrio cholerae to form biofilms may cause ineffectiveness of cholera treatment and prevention. The aim of the study was to determine the antibiotic sensitivity of V.cholerae in biofilms. Material and methods. Sensitivity to 11 antibacterial agents was determined for biofilms of 10 strains of V.cholerae of different serogroups isolated from humans and from water obtained for 48 hours at 37°C on plastic plates in vials with autoclaved tap water infected with a suspension of 104 V.cholerae microbial cells. For this purpose the plates were washed three times in physiological solution and transferred into penicillin vials with double dilutions of antibacterial agents in liquid nutrient medium (Martin's broth, pH 7.7). After 24 hours of cultivation in the thermostat (37°C), the biofilms were imprinted and 0.1 ml of plankton culture was seeded onto plates with Marten's agar (pH 7.7). After 24 hours in the thermostat (37°C), the biofilms were imprinted and 0.1 ml of plankton culture was sown on plates with Marten's agar (pH 7.7). The result was counted after 24 h, determining the minimum suppressive concentrations of preparations by the presence or absence of V.cholerae growth. Results. The strains studied in biofilm communities, in contrast to the planktonic form, were highly resistant to all antibacterial drugs taken in the study, except for ciprofloxacin and ceftazidime. To improve the effectiveness of treatment of cholera and other infections whose causative agents are cholera vibrio, it is necessary to determine the antibiotic sensitivity of V.cholerae biofilms.
About the Authors
N. A. Selyanskaya
Rostov-on-Don Antiplague Scientific Research Institute of Rospotrebnadzor
Russian Federation
Russian Federation
Selyanskaya Nadezhda Alexandrovna — Ph. D. in Medicine, Senior researcher department of microbiology of cholera and other acute intestinal infections
117/40 M. Gorky st., Rostov-on-Don, 344002
S. V. Titova
Rostov-on-Don Antiplague Scientific Research Institute of Rospotrebnadzor
Russian Federation
Russian Federation
Svetlana V. Titova — Ph. D. in Medicine, Leading researcher at the Laboratory of Natural Focal and Zoonotic Infections
Rostov-on-Don
E. A. Menshikova
Rostov-on-Don Antiplague Scientific Research Institute of Rospotrebnadzor
Russian Federation
Russian Federation
Elena A. Menshikova — Ph. D. in Biology, Senior researcher department of microbiology of cholera and other acute intestinal infections
Rostov-on-Don
V. D. Kruglikov
Rostov-on-Don Antiplague Scientific Research Institute of Rospotrebnadzor
Russian Federation
Russian Federation
Vladimir D. Kruglikov — D. Sc. in Medicine, Chief researcher department of microbiology of cholera and other acute intestinal infections
Rostov-on-Don
M. I. Yezhova
Rostov-on-Don Antiplague Scientific Research Institute of Rospotrebnadzor
Russian Federation
Russian Federation
Mariya I. Yezhova — Junior researcher department of microbiology of cholera and other acute intestinal infections
Rostov-on-Don
References
1. Romanova Jyu.M., Gintsburg A.L. Bakterial'nye bioplenki kak estestvennaya forma sushchestvovaniya bakterij v okruzhajyushchej srede i organizme khozyaina. Zhurnal Mikrobiologii. 2011; 3: 99–109. (in Russian)
2. Sadovskaya I., Vinogradov E., Li J. et al. High-level antibiotic resistance in Pseudomonas aeruginosa biofilm: the ndvB gene is involved in the production of highly glycerol-phosphorylated β-(1-3)-glucans, which bind aminoglycosides. Glycobiology. 2010; 20: 895–904. doi: 10.1093/glycob/cwq047. Epub 2010 Mar 26.
3. Farber B.F., Kaplan M.H., Clogston A.G. Staphylococcus epidermidis extracted slime inhibits the antimicrobial action of glycopeptides antibiotics. J Infect Dis. 1990; 161: 37–40. doi: 10.1093/infdis/161.1.37.
4. Mathur T., Singhal S., Khan S et al. Adverse effect of staphylococci slime on in vitro activity of glycopeptides. Jpn J Infect Dis. 2005; 58: 353–357.
5. Tatarenko O.A., Alekseeva L.P., Telesmanich N.R. i dr. Vliyanie nekotorykh faktorov na formirovanie bioplenki toksigennymi i atoksigennymi kholernymi vibrionami el'-tor. Epidemiologiya i Infektsionnye Bolezni. 2012; 5: 36–40. (in Russian)
6. Mayanskij A.N., Chebotar' I.V., Evteeva N.I., Rudneva E.I. Mezhvidovoe vzaimodejstvie bakterij i obrazovanie smeshannoj (polimikrobnoj) bioplenki. Zhurnal Mikrobiologii. 2012; 1: 93–101. (in Russian)
7. Hay A.J., Zhu J. Host intestinal signal-promoted biofilm dispersal induces Vibrio cholerae colonization. Infect Immun. 2015; 83 (1): 317–323. doi: 10.1128/IAI.02617-14. Epub 2014 Nov 3.
8. Titova S.V., Kushnareva E.V. Otsenka sposobnosti kholernykh vibrionov k obrazovanijyu bioplenok in vitro s pomoshch'jyu novogo metodicheskogo podkhoda. Fundamental'nye Issledovaniya. 2014; 10: 375–379. (in Russian)
9. Opredelenie chuvstvitel'nosti vozbuditelej opasnykh bakterial'nykh infektsij (chuma, sibirskaya yazva, kholera, tulyaremiya, brutsellez, sap, melioidoz) k antibakterial'nym preparatam. Metodicheskie Ukazaniya MU 4.2.2495-09. Moscow: 2009. (in Russian)
10. Sanitarno-epidemiologicheskie pravila SP 3.1.1086-02. Profilaktika kholery. Obshchie trebovaniya k epidemiologicheskomu nadzoru za kholeroj. Moscow: Federal'nyj Tsentr Gossanepidnadzora Minzdrava Rossii. 2002; 85. (in Russian)
11. Frolova Ya.N., Kharseeva G.G., Mironov A.Jyu. Chuvstvitel'nost' k antibiotikam bioplenochnykh kul'tur toksigennykh shtammov Corynebacterium diphtheriae. Klinicheskaya Laboratornaya Diagnostika. 2014; 59 (6): 51–53. (in Russian)
12. Chebotar' I.V., Mayanskij A.N., Konchakova E.D. i dr. Antibiotikorezistentnost' bioplenochnykh bakterij. Klinicheskaya Mikrobiologiya i Antimikrobnaya Khimioterapiya. 2012; 14 (1): 51–58. (in Russian)
13. Turkutjyukov V.B., Ibragimova T.D., Fomin D.V. Molekulyarnye osobennosti morfologii bioplenok, formiruemykh shtammami nefermentirujyushchikh gramnegativnykh bakterij. Tikhookeanskij Meditsinskij Zhurnal. 2013; 4: 44–47. (in Russian)
14. Vinnik Jyu.S., Serova E.V., Andreev R.I. i dr. Osobennosti formirovaniya mikrobnykh bioplenok na razlichnykh substratakh. Vozmozhnost' izucheniya bioplenok na zhelchnykh konkrementakh. Sovremennye Problemy Nauki i Obrazovaniya. 2013; 5: 1–8. (in Russian)
15. Harriott M.M., Noverr M.C. Candida albicans and Staphylococcus aureus form polymicrobial biofilms: effects on antimicrobial resistance. Antimicrob Agents Chemother. 2009; 53 (9): 3914–3922. doi: 10.1128/AAC.00657-09. Epub 2009 Jun 29.
16. Gabrielyan N.I., Gorskaya E.M., Romanova N.I., Tsirul'nikova O.M. Gospital'naya mikroflora i bioplenki. Vestnik transplantologii i iskusstvennykh organov. 2012; 14 (3): 83–91. (in Russian)
17. May T., Ito A., Okabe S. Induction of multidrug resistance mechanism in Escherichia biofilm by interplay between tetracycline and ampicillin resistance genes. Antimicrob Agents Chemother. 2009; 53 (11): 4628–39.
18. Pagel M., Simonet V., Li J. ey al. Phenotypic characterization of pore mutants of the Vibrio cholerae porin OmpU. J Bacteriol. 2007; 189 (23): 8593–600.
19. Golub A.V. Bakterial'nye bioplenki — novaya tsel' terapii? Klinicheskaya Mikrobiologiya I Antimikrobnaya Khimioterapiya. 2012; 14 (1): 23–29. (in Russian)
20. Luo Y., Ye J., Jin D. et al. Molecular analysis of non-O1/non-O139 Vibrio cholerae isolated from hospitalized patients in China. BMC Microbiol. 2013; 13: 52. doi: 10.1186/1471-2180-13-52.
21. George N., Fredrick F., Mohapatra A. et al. Non-O1, non-O139 Vibrio cholerae sepsis in a patient with nephrotic syndrome. Indian J Nephrol. 2013; 23 (5): 378–380. doi: 10.4103/0971-4065.116329.
Review
For citations:
Selyanskaya N.A., Titova S.V., Menshikova E.A., Kruglikov V.D., Yezhova M.I. Sensitivity to antibacterial drugs of biofilms formed by strains of vibrio cholerae of various serogroups. Antibiot Khimioter = Antibiotics and Chemotherapy. 2023;68(1-2):16-21. (In Russ.) https://doi.org/10.37489/0235-2990-2023-68-1-2-16-21
Views:
276
Email this article 