Modern Methods of Identification of Non-Tuberculous Mycobacteria
https://doi.org/10.37489/0235-2990-2024-69-9-10-86-90
EDN: MYZIBA
Abstract
Recently, an active increase in the incidence of non-tuberculous mycobacteriosis has been registered, which is determined not only by the improvement of methods for detecting non-tuberculous mycobacteria, but also by a fairly high level of awareness of medical personnel. The choice of the most accurate identification method is extremely important in determining the treatment tactics, since mycobacteria with different growth rates have pronounced differences in resistance to antimicrobial drugs. The emergence of molecular genetic research methods makes it possible to identify and type most of the currently known non-tuberculous mycobacteria, as well as determine their drug sensitivity. The prevalence of mycobacteriosis, which today represents a serious problem for both clinicians and laboratory specialists, requires the development of a new strategy for its microbiological diagnostics.
About the Authors
G. N. Genatullina
Astrakhan State Medical University
Russian Federation
Russian Federation
Guzel N. Genatullina — Ph. D. in Biology, Deputy Head of the Research Center, Associate Professor of the Department of Pharmacognosy, Pharmaceutical Technology, and Biotechnology
Astrakhan
A. L. Yasenyavskaya
Astrakhan State Medical University
Russian Federation
Russian Federation
Anna L. Yasenyavskaya — Ph. D. in Medicine, Associate Professor, Head of the Research Center, Associate Professor of the Department of Pharmacognosy, Pharmaceutical Technology, and Biotechnology
Astrakhan
M. A. Samotrueva
Astrakhan State Medical University
Russian Federation
Russian Federation
Marina A. Samotrueva — D. Sc. in Medicine, Professor, Head of the Department of Pharmacognosy, Pharmaceutical Technology, and Biotechnology
Astrakhan
References
1. Daley C. L., Griffith D. E. Pulmonary non-tuberculous mycobacterial infections. Int. J. Tuberc. Lung. Dis. 2010; 14 (6): 665–671.
2. Lyamin A. V., Zhestkov A. V., Ismatullin D. D., Kovalev A. M. Laboratornaya diagnostika mikobakteriozov. Vestnik Sovremennoj Klinicheskoj Mediciny. 2017; 10 (1): 29–35. doi: https://doi.org/10.20969/VSKM.2017.10(1).29-35. (in Russian)
3. Makarova M. V., Guntupova L. D. Netuberkuleznye mikobakterii. BIOpreparaty. Profilaktika, Diagnostika, Lechenie. 2020; 20(2): 97–102. doi: https://doi.org/10.30895/2221-996X-2020-20-2-97-102 (in Russian)
4. Burova, P. O. Sovremennye metody diagnostiki mikobakteriozov. FORCIPE. 2019; Prilozhenie, 595–596. (in Russian)
5. Labinskaya A. S., Kostyukova N. N. Opportunisticheskie infekcii: vozbuditeli i etiologicheskaya diagnostika. Moscow: Izd-vo «Binom» 2013; 752. (in Russian)
6. Zubairova P. Yu., Israpov I. M. Detekcii bakterial'nyh infekcij s pomoshch'yu DNK-zondov. Izvestiya Dagestanskogo Gosudarstvennogo Pedagogicheskogo Universiteta. Estestvennye i Tochnye Nauki. 2016; 10 (2): 34–40 (in Russian)
7. McNabb A., Eisler D., Adie K., Amos M., Rodrigues M., Stephens G., Black W. A., Isaac-Renton J. Assessment of partial sequencing of the 65- kilodalton heat shock protein gene (hsp65) for routine identification of mycobacterium species isolated from clinical sources. J Clin Microbiol. 2004; 42, 3000–3011. doi: 10.1128/JCM.42.7.3000-3011.2004.
8. Tortoli E. Impact of genotypic studies on mycobacterial taxonomy: the new mycobacteria of the 1990’s. J Clin Microbiol. Rev. 2003; 2: 319–354. doi: 10.1128/CMR.16.2.319-354.2003.
9. Hall L., Doerr K. A., Wohlfiel S. L., Roberts G. D. Evaluation of the MicroSeq system for identification of mycobacteria by 16S ribosomal DNA sequencing and its integration into a routine clinical mycobacteriology laboratory. J Clin Microbiol. 2003; 41: 1447–1453. doi: 10.1128/JCM.41.4.1447-1453.2003.
10. Voronina O. L., Kunda M. S., Lunin V. G., Gincburg A. L. Mul'tilokusnoe sekvenirovanie — informativnyj podhod molekulyarnoj ekologii. Vestnik Moskovskogo Universiteta. Seriya 16. Biologiya. 2010; 4: 7–10. (in Russian)
11. Chebotar' I. V., Polikarpova S. V., Bocharova Yu. A., Mayanskij N. A. Ispol'zovanie vremyaproletnoj massspektrometrii s matrichno-aktivirovannoj lazernoj desorbciej/ ionizaciej (MALDI-TOF MS) dlya identifikacii bakterial'nyh i gribkovyh vozbuditelej III–IV grupp patogennosti. Laboratornaya Sluzhba. 2018; 7 (2): 78–86. doi: https://doi.org/10.17116/labs20187278-86 (in Russian)
12. Huit G. A., Daley C. L. Nontuberculous mycobacteria. Clin Chest Med. 2015; 36 (1): xi–xii. doi: 10.1016/j.ccm.2014.11.006.
Review
For citations:
Genatullina G.N., Yasenyavskaya A.L., Samotrueva M.A. Modern Methods of Identification of Non-Tuberculous Mycobacteria. Antibiot Khimioter = Antibiotics and Chemotherapy. 2024;69(9-10):86-90. (In Russ.) https://doi.org/10.37489/0235-2990-2024-69-9-10-86-90. EDN: MYZIBA
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