Antimicrobial Activity of Aquipseudomonas alcaligenes Strain
https://doi.org/10.37489/02352990-2025-70-7-8-4-9
EDN: PVZQZO
Abstract
Background. The search for new effective antibiotics is one of the main tasks in the field of medicine, which is due to the spread of antibiotic-resistant pathogens that are not amenable to traditional chemotherapy.
The aim of the study was the search for a microorganism that could potentially produce an antibiotic exhibiting antimicrobial activity against pathogenic bacteria with multiple drug resistance.
The object of the study was a bacterium isolated from a freshwater indoor aquarium, identified as Aquipseudomonas alcaligenes and deposited in the collection of NIINA under the number INA 01568.
Materials and methods. For the surface cultivation of A. alcaligenes, bacterial and fungal strains, universal medium No. 2 Gause was used. Submerged cultivation was carried out in 18 nutrient media of different compositions. An agar diffusion method was used to determine the antimicrobial activity. Thirteen collection test strains of bacteria and fungi were used as test strains. Species identification was carried out by morphological features and the 16S rRNA gene.
Results. When cultured under submerged conditions on 2 media out of 18, the A. alcaligenes INA 01568 strain demonstrated antimicrobial activity against 6 test strains, including bacteria from the ESKAPE group, namely MRSA, Pseudomonas aeruginosa, as well as the vancomycin-resistant strain Leuconostoc mesenteroides; activity against these strains has been described for the first time. Activity against fungi was not detected.
Conclusion. The A. alcaligenes strain INA 01568 is promising for chemical research.
Keywords
Aquipseudomonas alcaligenes,
antimicrobial activity,
ESKAPE,
MRSA,
Leuconostoc mesenteroides,
Pseudomonas aeruginosa
Supporting agencies: The research was carried out within the state assignment of Ministry of Science and Higher Education of the Russian Federation (theme No. АААА-А18-118011650006-5)
About the Authors
I. A. Malanicheva
Gause Institute of New Antibiotics
Russian Federation
Competing Interests:
Russian Federation
Irina A. Malanicheva — Senior Researcher worker, Sector for Searching for Natural Compounds that Overcome Bacterial Resistance.
Moscow
Competing Interests:
The authors declare no conflict of interest
E. V. Khramtsova
Gause Institute of New Antibiotics
Russian Federation
Competing Interests:
Russian Federation
Ekaterina V. Khramtsova — Junior Researcher, Sector for Searching for Natural Compounds that Overcome Bacterial Resistance.
Moscow
Competing Interests:
The authors declare no conflict of interest
M. O. Makarova
Gause Institute of New Antibiotics
Russian Federation
Competing Interests:
Russian Federation
Marina O. Makarova — Engineer, Laboratory of Mutagenesis and Selection of Biologically Active Substance Producers.
Moscow
Competing Interests:
The authors declare no conflict of interest
O. V. Efremenkova
Gause Institute of New Antibiotics
Russian Federation
Competing Interests:
Russian Federation
Olga V. Efremenkova — Ph. D. in Biology, Head of the Sector, Sector for Searching for Natural Compounds that Overcome Bacterial Resistance.
Moscow
Competing Interests:
The authors declare no conflict of interest
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Review
For citations:
Malanicheva IA, Khramtsova EV, Makarova MO, Efremenkova OV. Antimicrobial Activity of Aquipseudomonas alcaligenes Strain. Antibiotiki i Khimioterapiya = Antibiotics and Chemotherapy. 2025;70(7-8):4-9. (In Russ.) https://doi.org/10.37489/02352990-2025-70-7-8-4-9. EDN: PVZQZO
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