Determination of Microbial Sensitivity to Polymyxin by the Method of Electroacoustic Analysis
Abstract
The influence of polymyxin on the change in the electrophysical parameters of a suspension of Escherichia coli cells of the K-12 strain was studied for the first time by electroacoustic analysis. It was shown that the maximum changes in the detected signal occur at a concentration of polymyxin of 25 μg/ml, furthermore they did not depend on the time of exposure to the antibiotic. The data obtained by the acoustic analysis method was confirmed by the standard microbiological method for determining microorganisms' sensitivity to polymyxin. The article shows the possibility of recording the sensitivity of microbial cells to antibacterial drugs and determining their antibacterial activity by the example of polymyxin with the use of the method of electroacoustic analysis of cell suspensions.
About the Authors
O. I. Guliy
Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences; Saratov State Vavilov Agrarian University; Saratov Scientific Research Veterinary Institute
Russian Federation
Russian Federation
B. D. Zaytsev
Saratov Branch of the Kotel'nikov Institute of Radio Engineering and Electronics of RAS
Russian Federation
Russian Federation
A. M. Shikhabudinov
Saratov Branch of the Kotel'nikov Institute of Radio Engineering and Electronics of RAS
Russian Federation
Russian Federation
I. A. Borodina
Saratov Branch of the Kotel'nikov Institute of Radio Engineering and Electronics of RAS
Russian Federation
Russian Federation
O. S. Larionova
Saratov State Vavilov Agrarian University
Russian Federation
Russian Federation
E. G. Zhnichkova
Saratov State Vavilov Agrarian University
Russian Federation
Russian Federation
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Review
For citations:
Guliy O.I., Zaytsev B.D., Shikhabudinov A.M., Borodina I.A., Larionova O.S., Zhnichkova E.G. Determination of Microbial Sensitivity to Polymyxin by the Method of Electroacoustic Analysis. Antibiot Khimioter = Antibiotics and Chemotherapy. 2017;62(3-4):3-9. (In Russ.)
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