Effect of Exogenic Proteolytic Enzymes on Transfer of Plasmid Genes in Mixed Bacterial Biofilms
Abstract
The effect of the enzyme complex from Vobenzin on transfer of plasmid genes In biofilms of gramnegative bacteria was studied. The extracellular matrix of the bacterial biofilms was shown to contain extracellular DNA carrying the antibiotic resistance markers. The action of the enzyme complex resulted in lower frequency of the antibiotic resistance genes transfer in mixed bacterial biofilms.
About the Authors
G. V. Tets
Academician I. P. Pavlov St.Petersburg State Medical University; St.Petersburg Academy of Postgraduate Medical Training
Russian Federation
Russian Federation
N. K. Artemenko
Academician I. P. Pavlov St.Petersburg State Medical University; St.Petersburg Academy of Postgraduate Medical Training
Russian Federation
Russian Federation
N. V. Zaslavskaya
Academician I. P. Pavlov St.Petersburg State Medical University; St.Petersburg Academy of Postgraduate Medical Training
Russian Federation
Russian Federation
K. L. Artemenko
Academician I. P. Pavlov St.Petersburg State Medical University; St.Petersburg Academy of Postgraduate Medical Training
Russian Federation
Russian Federation
G. YU. Knorring
Academician I. P. Pavlov St.Petersburg State Medical University; St.Petersburg Academy of Postgraduate Medical Training
Russian Federation
Russian Federation
V. V. Tets
Academician I. P. Pavlov St.Petersburg State Medical University; St.Petersburg Academy of Postgraduate Medical Training
Russian Federation
Russian Federation
YU. I. Sternin
Academician I. P. Pavlov St.Petersburg State Medical University; St.Petersburg Academy of Postgraduate Medical Training
Russian Federation
Russian Federation
References
1. Ghannoum M., O'Toole G. A., eds. Microbial Biofilms. Washington: ASM Press; 2004. p. 478.
2. Тец В. В. Бактериальные биоплёнки. Антимикробная терапия. Consilium medicum. 2007; Экстравыпуск: 13—15.
3. Tetz V. V., Korobov V. P., Artemenko N. K. et al. Extracellular phospholipids of isolated bacterial communities. Biofilms 2004; 1—7.
4. Li Y. H., Lau P. C., Lee J. H. et al. Natural genetic transformation of Streptococcus mutans growing in biofilms. J Bacteriol 2001; 183: 897—908.
5. Hausner M., Wuertz S. High rates of conjugation in bacterial biofilms as determined by quantitative in situ analysis. Applied Environmental Microbiol 1999; 65: 3710—3713.
6. Roberts A. P. Cheah G., Ready D. et al. Transfer ofTn916-like elements in microcosm dental plaques. Antimicrob Agents Chemother 2001; 45: 2943—2946.
7. Thoma C. M., Nielsen K. M. Mechanisms of and barriers to horizontal gene transfer between bacteria. Nature Rev 2005; 3: 711—721
8. Ghigo J. M. Natural conjugative plasmids induce bacterial biofilm development. Nature 2001; 25; 413: 6858: 772.
9. Монцевичуте-Эрингене E. В. Упрощенные математико-статистические методы в медицинской исследовательской работе. Патол Физиол Экспер Тер 1964; 4: 71—78.
10. Тец В. В., Кнорринг Г. Ю., Артеменко Н. К. и соавт. Влияние экзогенных протеолитических ферментов на бактерии. Антибиотики и химиотер 2004; 12: 3—6.
11. Tetz G. V., Artemenko N. K., Tetz V. V. Effect of DNase and antibiotics on biofilm characteristics. Antimicrob Agents Chemother 2009; 53: 1204—1209.
Review
For citations:
Tets G.V., Artemenko N.K., Zaslavskaya N.V., Artemenko K.L., Knorring G.Yu., Tets V.V., Sternin Yu.I. Effect of Exogenic Proteolytic Enzymes on Transfer of Plasmid Genes in Mixed Bacterial Biofilms. Antibiot Khimioter = Antibiotics and Chemotherapy. 2009;54(9-10):3-5. (In Russ.)
Views:
460
Email this article 