Activity of Carbohydrate Metabolism Enzymes in Streptomyces imbricatus, Producing Imbricin in Controlled Biosynthesis of the Antibiotic
Abstract
Changes in the fermentative activity of Streptomyces imbricatus under the conditions of stimulated biosynthesis of imbricin with addition of a crude endoregulator to the medium were studied. The activity of the two main enzymes of the glycolysis, i. e. phosphofructokinase and fructose-1,6-diphosphate aldolase (aldolase) was determined and the functioning of the pentozophosphate pathway was estimated by the activity of glucose-6-phosphate dehydrogenase and NADP-specific glutamate dehydrogenase. Under the conditions of the endoregulation the increase of the imbricin production was observed when the level of the glucose catabolism either by the glycolytic pathway or by the pentozophosphate pathway was comparatively high, thus providing the antibiotic biosynthesis with the required precursors and reducing equivalents.
About the Authors
O. V. Topkova
St. Petersburg State Chemico-Pharmaceutical Academy
Russian Federation
Russian Federation
E. P. Yakovleva
St. Petersburg State Chemico-Pharmaceutical Academy
Russian Federation
Russian Federation
V. A. Kolodyaznaya
St. Petersburg State Chemico-Pharmaceutical Academy
Russian Federation
Russian Federation
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Review
For citations:
Topkova O.V., Yakovleva E.P., Kolodyaznaya V.A. Activity of Carbohydrate Metabolism Enzymes in Streptomyces imbricatus, Producing Imbricin in Controlled Biosynthesis of the Antibiotic. Antibiot Khimioter = Antibiotics and Chemotherapy. 2010;55(3-4):3-7. (In Russ.)
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