Antiviral Activity of Aqueous Extracts and Polysaccharide Fractions from Mycelium and Fruit Bodies of Higher Fungi
Abstract
Sixty preparations of basidiomycetes ( Ganoderma, Lentinus, Pleurotus, Laetiporus, Polyporus, Inonotus, Flammulina, Grifola, Trametes) were investigated with respect to their toxicity for Vero cells and antiviral activity. The antiviral activity was estimated with the use of the West Nile virus and type 2 Herpes simplex. It was shown that 11 preparations of Ganoderma, Lentinus and Pleurotus completely inhibited the infective activity in doses not lower than 1000 TCD50 (the West Nile virus) and 100 PPU (type 2 Herpes simplex). The antiviral activity of the preparations was likely due to the content of polysaccharides or their derivatives in the composition. It increased with increasing of the quantity of the total polysaccharide fraction or its concentration.
About the Authors
I. A. Razumov
State Scientific Centre of Virology and Biotechnology VEKTOR, Koltsovo, Novosibirsk Region
Russian Federation
Russian Federation
T. A. Kosogova
State Scientific Centre of Virology and Biotechnology VEKTOR, Koltsovo, Novosibirsk Region
Russian Federation
Russian Federation
E. I. Kazachinskaya
State Scientific Centre of Virology and Biotechnology VEKTOR, Koltsovo, Novosibirsk Region
Russian Federation
Russian Federation
L. I. Puchkova
State Scientific Centre of Virology and Biotechnology VEKTOR, Koltsovo, Novosibirsk Region
Russian Federation
Russian Federation
N. S. Shcherbakova
State Scientific Centre of Virology and Biotechnology VEKTOR, Koltsovo, Novosibirsk Region
Russian Federation
Russian Federation
I. A. Gorbunova
State Scientific Centre of Virology and Biotechnology VEKTOR, Koltsovo, Novosibirsk Region
Russian Federation
Russian Federation
I. N. Mikhailovskaya
State Scientific Centre of Virology and Biotechnology VEKTOR, Koltsovo, Novosibirsk Region
Russian Federation
Russian Federation
V. B. Loktev
State Scientific Centre of Virology and Biotechnology VEKTOR, Koltsovo, Novosibirsk Region
Russian Federation
Russian Federation
T. V. Teplyakova
State Scientific Centre of Virology and Biotechnology VEKTOR, Koltsovo, Novosibirsk Region
Russian Federation
Russian Federation
References
1. Lederberg J. Infectious disease as an evolutionary paradigm. Emer Infect Dis 1997; 3: 4: 417-423.
2. http://www.who.int/whr/2008/en/index.html
3. Morens D. M., Folkers G. K., Fauci A. S. The challenge of emerging and re-emerging infectious diseases. Nature 2004; 430: 6996: 242-249.
4. Alter M. J. World epidemiology of hepatitis C virus infection. J Gastroenterol. 2007; 13: 17: 2436-2441.
5. Локтев В. Б. Вирус клещевого энцефалита. Генетические особенности и его изменчивость в современном мире. Бюллетень СО РАМН. 2007; 4: 14-21.
6. Белова Н. В. Перспективы использования биологически активных соединений высших базидиомицетов в России. Микология и фитопатология. 2004; 38: 2: 1-7.
7. Griffiths P. D. A perspective on antiviral resistance. J Clin Virol 2009; 46: 1: 3-8.
8. Успехи медицинской микологии / Под ред. Ю. В. Сергеева. 2003; 1: 1-321.
9. StametsP. MycoMedicinals / P. Stamets. An Informational Yreatise on Mushrooms: Printed in China, 2002; 96.
10. Brandt C. R., Pirano F. Mushroom antiviral. Recent Res Dev Antimicrob Agent Chemother 2000; 4: 11-26.
11. Piraino F., Brandt C. R. Isolation and partial characterization of an antiviral, RC-183, from the edible mushroom Rozites caperata. Antiviral Res 1999; 43: 2: 67-78.
12. Pirano F. F. The Development of the antiviral drug RC 28 from Rozites caperata (Pers.: Fr.) P. Karst. (Agaricomycetideae). Intern J Med Mushrooms 2005; 7: 356.
13. Gong M., Piraino F., Yan N. et al. Purification, partial characterization and molecular cloning of the novel antiviral protein RC 28. Peptides 2009; 30: 4: 654-659.
14. Wasser S. P. Medicinal mushrooms as a source of antitumor and immunomodulating polysaccharides. Appl Microbiol Biotechnol 2002; 60: 3: 258-274.
15. Ooi V. E. C., Liu F. Immunomodulation and anticancer activity of poly-saccharide-protein complex. Curr Med Chem 2000; 7: 7: 710-729.
16. Zaidman B. Z., Yassin M., Mahajna J., Wasser S. P. Medicinal mushroom modulators of molecular targets as cancer therapeutics. Appl Microbiol Biotechnol 2005; 67: 4: 453-468. Review.
17. Бухало A. С. Высшие съедобные базидиомицеты в чистой культуре. Киев: Наукова думка, 1988.
18. Теплякова Т. В. Биоэкологические аспекты хищных грибов-гифомицетов. 1999; 252.
19. Dibois M., Gilles K. A., Hamilton J. K. et al. Analytical Chemistry. 1956; 23: 3: 350-356.
20. Чхенкели В. A. Биологически активные вещества базидиомицета Corioluspubescens (Schum: Fr.) Quel. и их использование. РАСХН. Сиб.отд-ление. ИФ ИЭВС и ДВ. Новосибирск, 2006; 288.
21. Руководство по экспериментальному (доклиническому) изучению новых фармакологических веществ. М.: Минздрав РФ, 2000. 1-398.
22. Государственная фармакопея СССР// Общие методы анализа. Лекарственное растительное сырье. М., Медицина, 1990. 11 изд. вып. 2. том 2. 187-209.
23. Леннет Э., Шмидт Н. Лабораторная диагностика вирусных и риккетсиозных заболеваний. М.: 1974; 44-95.
24. Razumov I. A., Kazachinskaia E. I., Ternovoi V. A. et al. Neutralizing monoclonal antibodies against Russian strain of the West Nile virus. Viral Immunol 2005; 18: 3: 558-568.
25. Ломовский О. И. Прикладная механохимия: применение в пищевой промышленности и в сельском хозяйстве. Обработка дисперсных материалов и сред. Одесса, 2002; 12: 133-149.
Review
For citations:
Razumov I.A., Kosogova T.A., Kazachinskaya E.I., Puchkova L.I., Shcherbakova N.S., Gorbunova I.A., Mikhailovskaya I.N., Loktev V.B., Teplyakova T.V. Antiviral Activity of Aqueous Extracts and Polysaccharide Fractions from Mycelium and Fruit Bodies of Higher Fungi. Antibiot Khimioter = Antibiotics and Chemotherapy. 2010;55(9-10):14-18. (In Russ.)
Views:
360
Email this article 