Antibiotic Resistance of Coryneform Bacteria Isolated from the Reproductive Tract of Women
Abstract
The species composition, antilysozyme activity and antibiotic resistance of coryneform bacteria, isolated from the reproductive tract of women with microecological disturbances, were studied. Sixty six women without microecological disturbances and 102 female patients with microecological disturbances in the reproductive tract were examined. The study showed that Corynebacterium minutissimum, C.amycolatum, C.group JK, C.bovis and C.pseudodiphtheriticum prevailed in the healthy women. In the patients with microecological disturbances in the reproductive tract C.vitarumen, C.matruchotii, C.striatum, C.renale and C.urealyticum were detected in addition to the above species. The average antilysozyme activity of the coryneform bacteria isolated from the healthy women was 1.32+0.47. In the patients with microecological disturbances in the reproductive tract it was 1.84+0.38. The in vitro susceptibility of the coryneform bacteria to antimicrobial agents was determined. High prevalence of resistance to beta-lactams (oxacillin and penicillin), erythromycin and co-trimoxazole was detected. Thus, the species variety and the antilysozyme activity of the coryneform bacteria in the reproductive tract of the women with microecological disturbances were found to be higher. The high prevalence of resistance to oxacillin, penicillin, erythromycin and co-trimoxazole in the coryneform bacteria isolated from the patients with the microecological disturbances did not differ from that in the healthy women.
About the Authors
S. V. Cherkasov
Institute for Cellular and Intracellular Symbiosis, the Urals Branch of the Russian Academy of Sciences, Orenburg
Russian Federation
Russian Federation
I. V. Gladysheva
Institute for Cellular and Intracellular Symbiosis, the Urals Branch of the Russian Academy of Sciences, Orenburg
Russian Federation
Russian Federation
References
1. Кира Е. Ф. Бактериальный вагиноз. Спб., 2001; 364.
2. Анкирская А. С. Микроэкология влагалища и профилактика акушерской патологии. Инф и антимикроб химиотер 1999; 3.
3. Назарова Е. К., Гиммельфарб Е. И., Созаева Л. Г. Микробиоценоз влагалища и его нарушения. Этиология, патогенез, клиника, лабораторная диагностика. Антибиотики и химиотер 2002; 47: 4: 34-42
4. Мартикайнен 3. М. Коринебактерии женских гениталий (автореферат). Спб., 1996; 23.
5. Одинцова О. В. Коринеподобные и нокардиоподобные бактерии как компонент вагинального микробиоценоза (автореферат). Пермь. 2002; 23.
6. Funke G., von Graevenitz A., Clarridge III J. E., Bernard K. A. Clinical microbiology of coryneform bacteria. Clin Microbiol Rev 1997; 10: 125-159.
7. Coyle M. B., Lipsky B. A. Coryneform bacteria in infectious diseases: clinical and laboratory aspects. Clin Microbiol Rev 1990; 3: 227-246.
8. Bernard K. A., Munro C., Wiebe D. Characteristics of rare or recently described Corynebacterium species recovered from human clinical material in Canada. J. Clin. Microbiol 2002; 40: 11: 4375-4381.
9. Nebreda-Mayoral T., Munoz-Bellido J. L., Garcia-Rodriguez J. A. Incidence and characteristics of urinary tract infections caused by Corynebacterium urealyticum (Corynebacterium group D2). Eur J Clin Microbiol Infect Dis 1994; 13: 600-604.
10. Черкасов И. В. Характеристика микробиоценоза репродуктивного тракта женщин при трихомонадной инфекции (автореферат). Оренбург. 2009; 23.
11. Ferreira Camello T. C., Mattos-Guaraldi A. L., Formiga L. C. D. Nondiphtherial Corynebacterium species isolated from clinical specimens of patients in a university hospital, Rio De Janeiro, Brazil. Brazilian J. Microbiol 2003; 34: 39-44.
12. Aguado J. M., Ponte C., Soriano F. Bacteriuria with a multiple resistant species of Corynebacterium (Corynebacterium group D2): an unnoticed cause of urinary tract infection. J Infect Dis 1987; 156: 144-150.
13. Garcia-Rodriguez J. A., Garcia-Sanchez J. E., Munoz-Bellido J. L. In vitro activity of 79 antimicrobial agents against Corynebacterium group D2. Antimicrob. Agents Chemother 1991; 35: 2140-2143.
14. Philippon A., Bimet F. In vitro susceptibility of Corynebacterium group D2 and Corynebacterium jeikeium to twelve antibiotics. Eur J Clin Microbiol Infect Dis 1990; 9: 892-895.
15. Soriano F., Zapardiel J., Nieto E. Antimicrobial susceptibilities of Corynebacterium species and other non-spore-forming gram-positive bacilli to 18 antimicrobial agents. Antimicrob. Agents Chemother 1995; 39: 208F. 214.
16. Определитель бактерий Берджи в 2 томах / Под ред.Дж. Хоулта, Н. Крига, П. Снита и др.: Справочное издание. М.: 1997; 780.
17. Методические указания по определению чувствительности микроорганизмов к антибактериальным препаратам МУК 4.2.189004.2004. Клин микробиол антимикроб химиотер 2004; 6: 4.
18. Бухарин О. В. Персистенция патогенных бактерий. М.: 1999; 366.
19. Лакин Г. Ф. Биометрия. М.: Высшая школа, 1990.
20. Funke G., Lawson P. A., Collins M. D. Corynebacterium riegelii sp. nov., an unusual species isolated from female patients with urinary tract infections. J Clin Microbiol 1998; 36: 624-627.
21. Funke G., Pascual Ramos C., Collins M. D. Corynebacterium coyleae sp. nov., isolated from human clinical specimens. Int J Syst Bacteriol 1997; 47: 92-96.
22. Schiller J., Strom M., Groman N. Relationship between pNG2, an Emr plasmid in Corynebacterium diphtheriae, and plasmids in aerobic skin coryneforms. Antimicrob Agents Chemother 1983; 892-901.
23. Rosato A. E., Lee B. S., Nash K. A. Inducible macrolide resistance in Corynebacterium jeikeium. Antimicrob. Agents Chemother 2001; 45: 1982-1989.
24. Yague G., Mora B., Martinez-Toldos M. C., Rodriguez T. Implicacion de los genes erm X en la resistencia a los macrolidos y la telitromicina de Corynebacteriun jeiketum y Corynebacterium amycolatum. Rev Esp Quimioterap 2005; 18: 236-242.
Review
For citations:
Cherkasov S.V., Gladysheva I.V. Antibiotic Resistance of Coryneform Bacteria Isolated from the Reproductive Tract of Women. Antibiot Khimioter = Antibiotics and Chemotherapy. 2010;55(9-10):45-49. (In Russ.)
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