Microbiological and Molecular Genetic Characteristics of Coagulase-Negative Staphylococcal Isolates from Neonates in Intensive Care Unit
Abstract
The problem of hospital-acquired infections due to coagulase-negative staphylococci (CoNS) in neonatal intensive care units is crucial over the last 20 years in the world. Neonates with very low or extremely low body weight belong to a special group of risks by the CoNS infection. However, in Russia CoNS up to now are frequently considered as contaminants and not as the main etiologic factors of pneumonia and sepsis in extremely premature infants. It was shown that hospital strains of CoNS causing fatal infections in extremely premature infants are always present in intensive care units.
About the Authors
L. A. Lubasovskaya
V.I. Kulakov Scientific Centre of Obstetrics, Gynecology and Perinatology
Russian Federation
Russian Federation
M. A. Kornienko
Research Institute of Physico-Chemical Medicine
Russian Federation
Russian Federation
T. V. Priputnevich
V.I. Kulakov Scientific Centre of Obstetrics, Gynecology and Perinatology
Russian Federation
Russian Federation
E. N. Ilyina
Research Institute of Physico-Chemical Medicine
Russian Federation
Russian Federation
A. I. Shchegolev
V.I. Kulakov Scientific Centre of Obstetrics, Gynecology and Perinatology
Russian Federation
Russian Federation
References
1. Craft A., Finer N. Nosocomial coagulase negative staphylococcal (CoNS) catheter-related sepsis in preterm infants: definition, diagnosis, prophylaxis, and prevention. J Perinatol 2001; 26: 186-192.
2. Mohan P.V., Placencia F., Leonard E. W. Coagulase-negative staphylococcal infections in the neonate and child: an update. Semin Pediatr Infect Dis 2006; 17: 120-127.
3. Gruskay J., Harris M.C., Costarino A.T. et al. Neonatal Staphylococcus epidermidis meningitis with unremarkable CSF examination results. Am J Dis Child 1989; 143: 580-582.
4. Scheifele D.W., Gordean L. Bjornson Delta-like toxin produced by CoNS is associated with neonatal necrotizing enterocolitis. Infect Immun 1987; 2268-2273.
5. Eggink B.H., Rowen J.L. Primary osteomyelitis and suppurative arthritis caused by coagulase-negative staphylococci in a preterm neonate. Pediatr Infect Dis J 2003; 22: 572-573.
6. Haque N.Z., Zuniga L.C. et al. Relationship of vancomycin minimum inhibitory concentration to mortality in patients with methicillin-resistant Staphylococcus aureus hospital-acquired, ventilator-associated, or health-care-associated pneumonia. Chest 2010; 138: 6: 1356-1362.
7. Plata K., Rosato A., Wegrzyn G. Staphylococcus aureus as an infectious agent: overview of biochemistry and molecular genetics of its pathogenicity. Acta Biochim Pol 2009; 56: 4: 594-612.
8. Dinges M., Orwin P. Schlievert exotoxins of Staphylococcus aureus. PClin Microbiol Rev 2000; 13: 1: 13-34.
9. Otto M. Molecular basis of Staphylococcus epidermidis infections. Semin Immunopathol 2012; 34: 2: 201-214.
10. Marin M., de la Rose M., Cornejo I. Enterotoxigenicity of Staphylococcus strains isolated from Spanish dry-cured hams. Appl Environ Microbiol 1992; 58: 3: 1067-1069.
11. Madhusoodanan J., Seo K., Remortel B., Park J., Hwang S., Fox L., Park Y., Deobald C. An Enterotoxin-bearing pathogenicity island in Staphylococcus epidermidis. J Bacteriol 2011; 193: 8: 1854-1862.
12. Biofilms, infection, and antimicrobial therapy / Ed by J.L. Pace, M. Rupp, and R.G. Finch. Published by CRC Press Taylor & Francis Group, USA, 2006.
13. Menzies B.E. The role of fibronectin binding proteins in the pathogenesis of Staphybcoccus aureus infections. Curr Opin Infect Dis 2003; 16: 3: 225-229.
14. McDevitt D., Nanavaty T., House-Pompeo K., Bell E., Turner N., McIntire L., Foster T., Höök M. Characterization of the interaction between the Staphylococcus aureus clumping factor (ClfA) and fibrinogen. Eur J Biochem 1997; 247: 1: 416-424.
15. Ni Eidhin D., Perkins S., Francois P., Vaudaux P., Höök M., Foster T.J. Clumping factor B (ClfB), a new surface-located fibrinogen-binding adhesin of Staphylococcus aureus. Mol Microbiol 1998; 30: 2: 245-257.
16. Patti J.M., Jonsson H., Guss B., Switalski L.M., Wiberg K., Lindberg M., Höök M. Molecular characterization and expression of a gene encoding a Staphylococcus aureus collagen adhesin. J Biol Chem 1992; 267: 7: 4766-4772.
17. Vuong C., Kocianova S., Voyich J., Yao Y., Fischer E., DeLeo F., Otto M. A crucial role for exopolysaccharide modification in bacterial biofilm formation, immune evasion, and virulence. J Biol Chem 2004; 279: 52: 54881-54886.
18. Performance Standards for Antimicrobial Susceptibility Testing; Eighteenth Informational Supplement. CLSI Document M100-S18. 2008.
19. Isaacs D. A ten-year multicentre study of coagulase negative staphylococcal infections in Australasian neonatal units. Arch Dis Child 2003; 88: F89-93.
20. Bansal S., Jain A., Agarwal J., Malik G.K. Significance of coagulase negative staphylococci in neonates with late onset septicemia. Indian J Pathol Microbiol 2004; 47: 586-588.
21. Piette A., Verschraegen G. Role of coagulase-negative staphylococci in human disease. Vet Microbiol 2009; 134: 45-54.
22. Malachowa N., DeLeo F.R. Mobile genetic elements of Staphylococcus aureus. Cell Mol Life Sci 2010; 67: 18: 3057-3071.
23. Caiazza N. C., O'Toole G. Alpha-toxin is required for biofilm formation by Staphylococcus aureus. J Bacteriol 2003; 185:10: 3214-3217.
24. Воронина О.Л., Кунда М.С., Дмитриенко О.А., Лунин В.Г., Гинцбург А.Л. Разработка схемы мультилокусного секвенирования Staphylococcus haemolyticus и ее применение для молекулярно-эпидемиологического анализа штаммов, выделенных в стационарах Российской Федерации в 2009-2010 гг. Журн. микробиол. 2011; 5: 62-67.
25. Miragaia M., Thomas J.C., Couto I., Enright M.C., de Lencastre H. Inferring a population structure for Staphylococcus epidermidis from multilocus sequence typing data. J Bacteriology 2007; 189: 6: 2540-2552.
26. Li M., Wang X., Gao Q., Lu Y. Molecular characterization of Staphylococcus epidermidis strains isolated from a teaching hospital in Shanghai, China. J Med Microbiol 2009; 58: 4: 456-461.
27. Mendes R.E., Deshpande L.M., Costello A.J., Farrell D.J. Molecular epidemiology of Staphylococcus epidermidis clinical isolates from U.S. hospitals. Antimicrob Agents Chemother 2012; 56: 9: 4656-4661.
28. Krediet T.G., Mascini E.M., van Rooij E., Vlooswijk J., Paauw A., Gerards L.J., Fleer A. Molecular epidemiology of coagulase-negative staphylococci causing sepsis in a neonatal intensive care unit over an 11-year period. J Clin Microbiol 2004; 42: 3: 992-995.
29. Guilbert J., Meau-Petit V., de Labriolle-Vaylet C., Vu-Thien H., Renolleau S. Coagulase-negative staphylococcal osteomyelitis in preterm infants: a proposal for a diagnostic procedure. Arch Pediatr 2010; 17: 10: 1473-1476.
Review
For citations:
Lubasovskaya L.A., Kornienko M.A., Priputnevich T.V., Ilyina E.N., Shchegolev A.I. Microbiological and Molecular Genetic Characteristics of Coagulase-Negative Staphylococcal Isolates from Neonates in Intensive Care Unit. Antibiot Khimioter = Antibiotics and Chemotherapy. 2013;58(3-4):25-32. (In Russ.)
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