Biofilms of Antibiotic-Resistant Propionibacterium Acnes and Their Sensitivity to Antimicrobial Peptides of Staphylococci

The article Is devoted to the study of biological properties of antibiotic-resistant bacteria Propionibacterium acnes and their sensitivity to antibacterial cationic peptides varnerin and hominin. P.acnes Ac-1450 strain resistant to rifampicin and tetracycline were obtained by selection. With the help of the Kirby-Bauer disk diffusion test, it is shown that the acquisition of resistance to these antibiotics is accompanied by a decrease in the sensitivity of bacteria to a number of other antibacterial drugs. As a result of the determination of the minimum inhibitory concentrations of cationic peptides, it has been established that the sensitivity of antibiotic-resistant P.acnes strains to varnerin and hominin is maintained at the sensitivity level of the parent strain. Antibiotic-resistant bacteria show a more pronounced ability for adhesion and biofilm formation in comparison with the bacteria of the parent strain, however, the formation of biofilms can be effectively suppressed by staphylococcins. Analysis of the curves of the formation intensity of P.acnes biofilms, depending on the content of peptides in the medium, made it possible to establish concentrations that inhibit the growth of biofilms by 50%. Despite the fact that the values obtained were 5-12 times higher than the MIC values for planktonic culture of P.acnes, staphylococcins are promising drugs for combating infections caused by propionic bacteria.

Propionibacterium acnes, Propionibacterium acnes, antibiotic resistance, rifampicin, tetracycline, biofilms, adhesion, antibacterial peptides, staphylococcins

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