Molecular Сharacterization of Neisseria meningitidis Serogroup W in St. Petersburg
https://doi.org/10.37489/0235-2990-2022-67-5-6-14-18
Abstract
The study included 14 isolates of Neisseria meningitidis serogroup W, isolated from patients with generalized forms of meningococcal infection aged 0–17 years at the Pediatric Research and Clinical Center for Infectious Diseases during the period from 2009 to 2020, as well as from carriers. The isolates from carriers (17–18 years old) were isolated in 2016–2019. The isolates were identified by MALDI-TOF mass spectrometry using a Microflex LT mass spectrometer (Bruker Daltonics, Germany). Whole genome sequencing of N. meningitidis was carried out on the basis of the Miseq Illumina platform; it was established that they belong to ST11 (cc11) sequence type. The results of the whole genome sequencing of the studied meningococci showed that there are 2 groups of the subcluster belonging to the Hajj cluster in St. Petersburg. In addition, they show close affinity with meningococci of the Anglo-French and Swedish clusters of the Hajj line.
About the Authors
E. A. MARTENS
Pediatric Research and Clinical Center for Infectious Diseases under the Federal Medical Biological Agency; North-Western State Medical University named after I. I. Mechnikov
Russian Federation
Russian Federation
Elvira A. Martens — Head of the Clinical Diagnostic Laboratory, Junior Researcher, Pediatric Research and Clinical Center for Infectious Diseases under the Federal Medical Biological Agency; Assistant at the Department of Medical Microbiology North-Western State Medical University named after I. I. Mechnikov.
WOS Researcher ID: CAG-4447-2022
Scopus Author ID: 57206470215
Saint Petersburg
L. I. ZHELEZOVA
Pediatric Research and Clinical Center for Infectious Diseases under the Federal Medical Biological Agency
Russian Federation
Russian Federation
Lyudmila I. Zhelezova — Ph. D. in medicine, Pediatric Research and Clinical Center for Infectious Diseases.
WOS Researcher ID: G-9662- 2012
Scopus Author ID: 6505716185
Saint Petersburg
V. V. GOSTEV
Pediatric Research and Clinical Center for Infectious Diseases under the Federal Medical Biological Agency; North-Western State Medical University named after I. I. Mechnikov
Russian Federation
Russian Federation
Vladimir V. Gostev — Ph. D. in biology, Pediatric Research and Clinical Center for Infectious Diseases under the Federal Medical Biological Agency, North-Western State Medical University named after I. I. Mechnikov.
WOS Researcher ID: P-1949-2016
WOS Researcher ID: 55614534400
Saint Petersburg
D. V. LIKHOLETOVA
Pediatric Research and Clinical Center for Infectious Diseases under the Federal Medical Biological Agency
Russian Federation
Russian Federation
Daria V. Likholetova — Laboratory researcher at the Department of Medical Microbiology and Molecular Epidemiology
Saint Petersburg
S. M. ZAKHARENKO
Pediatric Research and Clinical Center for Infectious Diseases under the Federal Medical Biological Agency
Russian Federation
Russian Federation
Sergey M. Zakharenko — Ph. D. in medicine, associate professor
SCOPUS: 6701402980.
Saint Petersburg
References
1. Maiden M.C., Bygraves J.A., Feil E., Morelli G, Russell J.E., Urwin R., Zhang Q., Zhou J., Zurth K., Caugant D.A. et al. Multilocus sequence typing: a portable approach to the identification of clones within populations of pathogenic microorganisms. Proc Natl Acad Sci U S A 1998, 95: 3140–3145. doi:10.1073/pnas.95.6.3140.
2. Yazdankhah S.P., Kriz P., Tzanakaki G., Kremastinou J., Kalmusova J., Musilek M., Alvestad T., Jolley K.A., Wilson D.J., McCarthy N.D. et al. Distribution of serogroups and genotypes among disease-associated and carried isolates of Neisseria meningitidis from the Czech Republic, Greece, and Norway. J Clin Microbiol. 2004; 42: 5146–5153. doi:10.1128/JCM.42.11.5146-5153.2004.
3. Stephens D.S., Greenwood B., Brandtzaeg P. Epidemic meningitis, meningococcaemia, and Neisseria meningitidis. Lancet. 2007; 369: 2196–2210. doi:10.1016/S0140-6736(07)61016-2.
4. Daugla D.M., Gami J.P., Gamougam K., Naibei N., Mbainadji L., Narbe M., Toralta J., Kodbesse B., Ngadoua C., Coldiron M.E. et al. Effect of a serogroup A meningococcal conjugate vaccine (PsA-TT) on serogroup A meningococcal meningitis and carriage in Chad: a community study [corrected]. Lancet. 2014; 383: 40–47. doi:10.1016/S0140-6736(13)61612-8. Epub 2013 Sep 12.
5. Gamougam K., Daugla D.M., Toralta J., Ngadoua C., Fermon F, Page AL, Djingarey M.H., Caugant D.A., Manigart O., Trotter C.L. et al. Continuing effectiveness of serogroup A meningococcal conjugate vaccine, Chad, 2013. Emerg Infect Dis. 2015; 21: 115–118.
6. Tzeng Y.L., Stephens D.S. A Narrative Review of the W, X, Y, E, and NG of Meningococcal Disease: Emerging Capsular Groups, Pathotypes, and Global Control. Microorganisms. 2021, 9 (3): 519. doi:10.3390/microorganisms9030519.
7. Caugant D.A., Brynildsrud O.B. Neisseria meningitidis: using genomics to understand diversity, evolution and pathogenesis. Nat Rev Microbiol. 2020; 18: 84–96. doi:10.1038/s41579-019-0282-6.
8. Lucidarme J., Hill D.M., Bratcher H.B., Gray S.J., du Plessis M., Tsang R.S., Vazquez J.A., Taha M.K., Ceyhan M., Efron A.M. et al. Genomic resolution of an aggressive, widespread, diverse and expanding meningococcal serogroup B, C and W lineage. J Infect 2015, 71: 544–552.
9. Caugant D.A., Brynildsrud O.B. Neisseria meningitidis: using genomics to understand diversity, evolution and pathogenesis. Nat Rev Microbiol 2020, 18: 84–96. doi:10.1038/s41579-019-0282-6.
10. Mayer L.W., Reeves M.W., Al-Hamdan N., Sacchi C.T., Taha M.K., Ajello G.W., Schmink S.E., Noble C.A., Tondella M.L., Whitney A.M. et al. Outbreak of W135 meningococcal disease in 2000: not emergence of a new W135 strain but clonal expansion within the electophoretic type-37 complex. J Infect Dis. 2002; 185: 1596–1605. doi:10.1086/340414.
11. Fazio C., Neri A., Vacca P., Ciammaruconi A., Arghittu M., Barbui A.M., Vocale C., Bernaschi P., Isola P., Galanti I.A. et al. Cocirculation of Hajj and non-Hajj strains among serogroup W meningococci in Italy, 2000 to 2016. Euro Surveill. 2019; 24 (4): 1800183. doi:10.2807/1560-7917.ES.2019.24.4.1800183.
12. Mustapha M.M., Marsh J.W., Harrison L.H. Global epidemiology of capsular group W meningococcal disease (1970–2015): Multifocal emergence and persistence of hypervirulent sequence type (ST)-11 clonal complex. Vaccine 2016, 34: 1515–1523. doi:10.1016/j.vaccine.2016.02.014.
13. Lucidarme J., Scott K.J., Ure R., Smith A.J., Lindsay D.S.J., Stenmark B., Jacobsson S., Fredlund H., Cameron J.C., Smith-Palmer A. et al. An international invasive meningococcal disease outbreak due to a novel and rapidly expanding serogroup W strain, Scotland and Sweden, July to August 2015. Euro Surveil. 2016; 21 (45): 30395. doi:10.2807/1560-7917.ES.2016.21.45.30395.
14. Ladhani S.N., Beebeejaun K., Lucidarme J., Campbell H., Gray S., Kaczmarski E., Ramsay M.E., Borrow R. Increase in Endemic Neisseria meningitidis Capsular Group W Sequence Type 11 Complex Associated With Severe Invasive Disease in England and Wales. Clin Infect Dis. 2014; 60 (4): 578–585. doi:10.1093/cid/ciu881. Epub 2014 Nov 10.
15. Lemos A.P., Gorla M.C., de Moraes C., Willemann M.C.A., Sacchi C.T., Fukasawa L.O., Camargo C.H., Barreto G., Rodrigues D.S., Gonçalves M.G. et al. Emergence of Neisseria meningitidis W South American sublineage strain variant in Brazil: disease and carriage. J Med Microbiol. 2022; 71 (2). doi:10.1099/jmm.0.001484.
16. Wick R.R., Judd L.M., Gorrie C.L., Holt K.E. Unicycler: Resolving bacterial genome assemblies from short and long sequencing reads. PLoS Comput Biol. 2017; 13:e1005595. doi:10.1371/journal.pcbi.1005595.
17. Seemann T. Prokka: rapid prokaryotic genome annotation. Bioinformatics. 2014; 30: 2068–2069. doi:10.1093/bioinformatics/btu153
18. Gardner S.N., Slezak T., Hall B.G. kSNP3.0: SNP detection and phylogenetic analysis of genomes without genome alignment or reference genome. Bioinformatics. 2015; 31 (17): 2877–2878. doi:10.1093/bioinformatics/btv271.
19. https://pubmlst.org/
Review
For citations:
MARTENS E.A., ZHELEZOVA L.I., GOSTEV V.V., LIKHOLETOVA D.V., ZAKHARENKO S.M. Molecular Сharacterization of Neisseria meningitidis Serogroup W in St. Petersburg. Antibiot Khimioter = Antibiotics and Chemotherapy. 2022;67(5-6):14-18. (In Russ.) https://doi.org/10.37489/0235-2990-2022-67-5-6-14-18
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