Development of the screening conditions in vitro effective drugs against the causative agent of Сhikungunya fever
S. Ya. Loginova,
V. N. Schukina,
S. V. Savenko,
R. V. Sakharov,
I. V. Surovyatkina,
S. V. Borisevich https://doi.org/10.37489/0235-2990-2023-68-1-2-11-15
Abstract
The Сhikungunya virus (CHIKV) is a member of the Flavivirus genus, the Flaviviridae family, and is a zoonotic arbovirus infection transmitted by Aedes mosquitoes. Chikungunya fever causes explosive epidemics involving millions of cases. There is an urgent need to develop broad-spectrum antivirals. Actual is the development of a model of screening in vitro effective drugs. To select the optimal screening conditions, a comparative analysis of cell cultures sensitivity to Сhikungunya virus, strain FN198/66 was carried out. The dynamics and level of virus accumulation at different infectious doses were estimated. It is shown that the Сhikungunya virus, strain FN198/66, well propagated in transplantable cell cultures Vero V, Сl008 Vero, BHK-21/13 and TC-17. All studied cell lines can be used to screen for effective non-specific medical defenses against Сhikungunya virus.
About the Authors
S. Ya. Loginova
48th Central Scientific Research Institute of the Ministry of Defence of the Russian Federation
Russian Federation
Competing Interests:
Russian Federation
Svetlana Ya. Loginova — D. Sc. in Biology, Leading researcher
Sergiev Posad
Competing Interests:
None
V. N. Schukina
48th Central Scientific Research Institute of the Ministry of Defence of the Russian Federation
Russian Federation
Competing Interests:
Russian Federation
Veronika N. Schukina — Ph. D. in Biology, Researcher
Sergiev Posad
Competing Interests:
None
S. V. Savenko
48th Central Scientific Research Institute of the Ministry of Defence of the Russian Federation
Russian Federation
Competing Interests:
Russian Federation
Sergey V. Savenko — Senior researcher
Sergiev Posad
Competing Interests:
None
R. V. Sakharov
48th Central Scientific Research Institute of the Ministry of Defence of the Russian Federation
Russian Federation
Competing Interests:
Russian Federation
Roman V. Sakharov — Senior researcher
Sergiev Posad
Competing Interests:
None
I. V. Surovyatkina
48th Central Scientific Research Institute of the Ministry of Defence of the Russian Federation
Russian Federation
Competing Interests:
Russian Federation
Surovyatkina Irina Vladimirovna — Ph. D. in Biology, Researcher
Sergiev Posad
Competing Interests:
None
S. V. Borisevich
48th Central Scientific Research Institute of the Ministry of Defence of the Russian Federation
Russian Federation
Competing Interests:
Russian Federation
Sergey V. Borisevich — D. Sc. in Biology, Professor, Academician of the Russian Academy of Sciences, Head of the Institute
Sergiev Posad
Competing Interests:
None
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Review
For citations:
Loginova S.Ya., Schukina V.N., Savenko S.V., Sakharov R.V., Surovyatkina I.V., Borisevich S.V. Development of the screening conditions in vitro effective drugs against the causative agent of Сhikungunya fever. Antibiot Khimioter = Antibiotics and Chemotherapy. 2023;68(1-2):11-15. (In Russ.) https://doi.org/10.37489/0235-2990-2023-68-1-2-11-15
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