Given the lack of specific antiviral medicines to treat dengue fever, finding new medicinal products, including natural products, remains a highly pertinent goal. The objective of the study was to determine the antiviral activity of Influcid against dengue virus replication in vitro. Material and methods. Dengue virus DENV-2/RUS/TH-Novosibirsk02/2012 subtype 2 was used in this study. Vero cells were infected with dengue virus. Reduction of cytopathic effect and virus accumulation in culture fluid with the influence Influcid has been studied. Results and conclusions. The 50% inhibiting concentration of Influcid was determined as 58 mg/ml. The results obtained from these in vitro investigations show that Influcid is able to effectively inhibit dengue virus (subtype 2) replication in Vero cells. Since Influcid has a favorable benefit-risk profile it might be an option that can be used as treatment in addition to conventional medicine in patients suffering from dengue fever. The transferability of in vitro data to humans must show further investigations.

It Is shown that the preliminary administration of drugs that promote the flow of antibiotics into the lymphatic system increases the clinical effectiveness of treatment. Currently, there is a need for experimental justification of this approach to antibacterial therapy. In this study, the effect of drugs with hyaluronidase activity on the rate of lymphatic tissue drainage and the pharmacokinetics of cefotaxime was studied. We determined the time of the removal of lymphotropic blue dye evans of the mesentery of mice when administered on the background of hyaluronidase and bovhyaluronidaze azoximer, the concentration of cefotaxime in the dynamics during the day in blood plasma of rabbits and in the blood plasma, the intestinal tissues and the liver of mice after 1.5 and 24 h after the co-administration of antibiotic with hyaluronidase and bolgelerinde anoxemia. It is found that the hyaluronidase, and bovhyaluronidaze azoximer are equieffective stimulants of lymphatic drainage of tissues. The data obtained indicate the presence of endolymphatic properties of the conductor not only on hyaluronidase, but at bovhyaluronidaze azoximer, additional properties which allow to consider it as a priority of the drug when conducting lymphotropic therapy.

Studies have been carried out to study the effectiveness of the high-molecular inducer of interferon Larifan® in relation to the experimental form of severe acute respiratory syndrome in Syrian hamsters. It is shown that Lariphan® is effective when applied in a preventive scheme, and the scheme of emergency prevention. The coefficient of therapeutic action for virological, hematological and biochemical parameters was 57.5 and 65.0%, respectively (ð<0.05 and ð<0.01).

Determined the primary chemoresistance markers (expression of genes ERCC1, TOP2a and ABCB1) in epithelial cells of ascites 32 primary patients with verified ovarian cancer using RT-PCR. After standard chemotherapy by the AR scheme (cisplatin+doxoru-bicin), the relapse-free survival of patients was assessed by Kaplan-Mayer. Patients with early (up to 6 months) and late (more than 6 months) relapse were isolated. As a result of the conducted studies it was established that early relapse occurred in 67% of the examined patients (the relapse-free period was 88.7+13.3 days). At the same time, in the groups with increased expression, the relapse-free period did not differ significantly, there were early relapses. The use of the Cochrane Q-test has allowed to establish that the reduced levels of ERCC1 and ABCB1, the proportion of survivors is to 72.7% (p<0.157 inch); decrease in ERCC1 and increased TOP2a increases the probability of survival to 75%; reduction of ERCC1 and reduced TOP2a determines the probability of survival of 69.2% (p<0.157 inch). Therefore, to predict the possible response to standard chemotherapy by the AR scheme in ascitic ovarian cancer, it is advisable in the epithelial cells of ascites to evaluate the expression of 2 parameters - ERCC1 and ABCB1.

The objective of this study was to clarify the intraspecies phylogenetic relationship between pneumococci strains from various geographical locations. Concatenated Multilocus sequence typing (MLST) sequences of six housekeeping genes were chosen for the phylogenetic tree construction. Trees, constructed for concatenated genes, revealed that there is a tendency for a clonal spread of antibiotic resistance. Interestingly, the isolates with novel or circulating mainly in Russia genotypes, described in this study, many are related to successful international clones, with the potential to spread.

The efficacy of fucoidan from brown algae of the Okhotsk sea Fucus evanescens in vaccination against seasonal influenza in people requiring priority protection due to the high risk of disease and the development of post-infective complications (elderly people) was studied. 40 participants aged 57 to 82 years participated in a number of ways, starting from the day of vaccination (vaccine «Sovigripp») in the dose 100 mg 2 times a day for 30 days. Through 5 weeks after vaccination, the immune response to strains A/H1N1 and a/H3N2 in the elderly control group did not meet the criteria of the European Committee on patented drugs, the concentration of antibodies in serum did not differ from the baseline. In the group of participants taking fucoidan, the level of seroconversion and seroprotection was higher than that in the control group, the mean geometric titer (SGT) of antibodies to all 3 strains significantly exceeded that before vaccination. SGT of antibodies to influenza b virus after vaccination in the experimental group significantly exceeded the parameters of the control group. In titer-stratified pre-vaccination antibodies (<1:40) in the experimental group, the seroconversion coefficient and level to strains A/H1N1 and a/H3N2 were higher than those in the control group and reached the European Committee criteria for A/H3N2 (2.5). The level of seroprotection in stratified participants to all three strains was also higher in the experimental group than in the control group. For the a/H1N1 virus, the European Committee criteria were not met in both groups. Fucoidan from brown algae of the Okhotsk sea Fucus evanescens can be used to improve the efficacy of vaccination against seasonal influenza in the elderly. To confirm the findings, it is necessary to conduct further studies on a large clinical material with an assessment of the functional activity of cells of the immune system and the cytokine system.

On the basis of the examination and treatment of 446 patients with carbuncles, it was found that the main infection germ is Staphylococcus aureus mainly in the form of monoculture. Among the singled out strains multidrugsresistant forms (MRSA) reached 13,8%. The most severe course of carbuncles was observed in patients with diabetes mellitus, but the true difference in frequency of methicillin-sensitive and methicillin-resistant staphylococcus release wasn't observed. All singled out strains MRSA have a high sensitivity to the vancomycin and linezolid. Much less frequently (9,8%) Streptococcus of group A was sown antibiotic resistance wasn't found. Microbical landscape of postcritically wounds was changing in the course of 3-4 days. Streptococcus releases such gram-negative microflora among this strains more than the half was characterized by multiantibioticresistance. Contamination of the postcritically wounds in patients that suffers from diabetes mellitus, with hospital microflora was much frequent than in patients without diabetes mellitus, frequency of the excretion of microbical associations are higher in those with diabetes (47,6%). In such situations the negative dynamic of the wound process. It shows the necessity of conduction of staged necrectomies and changes in antibacterial therapy regimens adjusted to the sensitivity of the singled out microflora.

The aim of this study was to evaluate effectiveness of remaxol inclusion in the supporting therapy regimen in patients with the fibrous-cavernous form of drug-resistant tuberculosis. Treatment of 173 patients with fibrous-cavernous pulmonary tuberculosis was analyzed. Standard clinical laboratory methods and x-ray diagnostic were conducted in all patients as well as the spectrum of pathogen sensitivity to anti-tuberculosis drugs was defined. Changes in immune status and state of liver detoxification function were evaluated in dynamics (before and after hepatoprotective therapy course) taking in consideration level of cytokines (interleukins 1,4,6 and 10, tumor necrosis factor, interferon-gamma) and liver enzymes (AST, ALT). Two groups of patients were formed using randomization: group 1 (main) with 111 patients who received etiotropic therapy and complex of treatment and rehabilitation activities, and group 2 (comparisons) with 62 patients who, in addition to the main complex of therapy, received remaxol: intravenously, 400 ml once a day, a course of 12 droppers. In this research it was revealed that remaxol inclusion in the supporting treatment regimen showed hepatoprotective (improved transaminase) and minor immunoprotective (positive cytokine profile) effects. The obtained laboratory results correlate with clinical data: prevalence of high (53.8%) and absence of low treatment efficacy cases and a higher degree of patients adherence to treatment.

When carrying out professional activities of specialists in mountainous conditions associated with physical and psycho-emotional stress, measures aimed at adapting the body to high altitude hypoxia, such as conducting hypoxic training both in natural mountainous areas and modeling hypoxic hypoxia using hypobaric chambers or hypoxicators simulating normobaric hypoxia and the use of pharmacological correction that allows ro mobilize either economize resources of the organism. The antihypoxant / antioxidant cytoflavin, successfully used in the treatment of diseases of the nervous system, is supposed to be used to improve physical performance and psychomotor functions among professionals working in mountainous areas. The authors conducted a pilot study with the participation of climbers, who completed a course of hypoxic hypobaric training with the pharmacocorrection of trecresan and cytoflavin. Revealed a tendency to increase the level and concentration of attention in athletes who received pharmacocorrection, in contrast to taking placebo. The identified positive trends in psychomotor performance combined with good tolerability of the drug are the basis for planning further research.

The results of Global Point Prevalence Survey of Antimicrobial Consumption and Resistance (GLOBAL-PPS) project conducted in 7 multi-field hospitals in different cities of Russia (Krasnodar, Moscow, Omsk, Smolensk, Tomsk, Ulan-Ude) during the period from September to November 2017 are presented. Case records of patients who received systemic antimicrobial agents for the treatment or prevention of infections were analyzed. Local practices of antimicrobials administration were evaluated with help of quality indicators defined in GLOBAL-PPS study protocol. Common mistakes requiring a prompt intervention included low adherence to clinical guidelines, absence of stop/review dates in the medical records, extended duration of surgical prophylaxis, III generation cephalosporin's overuse for prophylaxis and treatment and low frequency of therapy based on microbiological data.Our data provide the basis for the development of appropriate antimicrobial stewardship programs tailored according to local practices for each multi-field hospital in the project.

The problem of antibiotic resistance of pathogenic bacteria is considered, data on antibiotics introduced into medical practice since 2000 in the world, information about existing programs and strategies to prevention antibiotic resistance are given.

Antibiotic resistance is one of the world problems of the XXI century, resulting from the activation of adaptive mechanisms in the structures of bacterial cells. Secondary metabolites of plants are synthesized as byproducts of their metabolism. The release of secondary metabolites into the environment is associated with the activation of protective mechanisms in plant cells. In the course of the study of the literature data the following mechanisms of antibacterial action of secondary metabolites of plants were identified: mechanisms of damage to the cell wall and cytoplasmic membrane of bacteria, violations of energy metabolism and metabolic processes, as well as DNA synthesis. The principles of the systems «metabolite-metabolite» and «metabolite-antibiotic» are generalized, which makes it possible to judge the possibility of potentiating the action of antibiotics by secondary metabolites of plants.

Currently, Immunosuppressive therapy Is becoming Increasingly common In the curation of patients with systemic rheumatic diseases (RH). However, the active use of immunosuppressors, including genetically engineered biological drugs is accompanied by an increase in opportunistic infections. The latter include pneumocystic pneumonia (PPn), which is a serious complication with significant mortality in patients with RA. Taking into account the heterogeneous risk data for specific RS and a particular immunosuppressive therapy, the establishment of evidence-based comprehensive guidelines for the prevention of PPn in rheumatology is not currently possible. Some guidelines for practicing physicians, published different collectives, can serve algorithms for the prevention of PPn, which will no doubt be further refined (or processed) the accumulation of new data.