Comparative antimicrobial activity of lactoferrins from various sources (native lactoferrin from Laprot, human hololactoferrin, recombinant human lactoferrin isolated from the cultural medium of permissive cell culture transfected using pseudoadenovirus nanostructure with the human lactoferrin gene, and native bovine lactoferrin) was studied to prove the possibility of their use for development of antimicrobial drugs. It was shown that all the substances were active against the Bacillus standard strains. The antibacterial activity was almost independent of the degree of saturation the lactoferrin molecules with Fe³⁺. The native human lactoferrin was more active than hololactoferrin against Candida when evaluated by the minimum inhibitory concentration (MIC). Fe³⁺-Non aturated recombinant human lactoferrin demonstrated the antimicrobial activity (by MIC) similar to that of the native human lactoferrin. The results showed that native and recombinant human lactoferrins might be used for the development of intravenous and intracavitary dosage forms, while the native bovine lactoferrin could be useful in development of oral drugs.

The experimental study of the Ingavirin® efficacy against the influenza virus A (H5N1) on intranasally-infected albino mice vs. Tamiflue® and Arbidol® showed that when used for the prophylaxis, urgent prophylaxis and therapy it was effective in the protec-tiom of the animals from death. The efficient dose for the prophylaxis of the influenza infection was 5 mg/kg (protective efficacy of 46.7%) and for the urgent prophylaxis and therapy it was 15 mg/kg (protective efficacy of 40.0 and 35.0% respectively).

The Ingavirin® antiviral properties with respect to the parainfluenza virus, as an actual human respiratory tract pathogen, were investigated by two methods, i.e. immunoenzymatic analysis and microtetrazolium test. The results showed that along with the immediate antiviral activity Ingavirin® had nonspecific cytoprotective properties. While affecting the virus proteins synthesis, Ingavirin^® lowered the virus cytopathogenic action. The drug significantly decreased the portion of the bronchial epithelium cells killed at the stage of acute infection.

Luromarin, extracted from the sea alga Zostera asiatica, showed no cytotoxic activity when incubated with the SPEV cell culture and was characterized by virulicidal properties against highly pathogenic strain of TBE virus and the ability to inhibit the virus replication at the early stages of the infection.

In vitro synergistic antiviral effect of interferon α-2b in combination with unithiol (an antioxidant) on diverse variants of Herpes simplex, including the clinical acyclovir-resistant isolates, was demonstrated. Unithiol showed immediate antiherpes activity in the cell culture. However, its activity was lower than that of ascorbic acid, another highly active antioxidant. It was also observed that the specific antiherpes activity of interferon α-2b in the presence of unithiol increased several times. The data could be useful in the clinical practice, since the drugs combinations or complex formulations provide higher efficacy of the herpetic infection therapy with the use of the same concentrations of the available agents.

To investigate the hemodynamics and myocardic contraction of the heart left ventricle, 61 patients with pulmonary tuberculosis (main group) and 26 healthy subjects (control group) were observed. Higher ultimate systolic and diasystolic volumes of the left ventricle and lower levels of the efflux fraction in the patients with active pulmonary tuberculosis were stated. There was shown inverse correlation of the systemic systolic arterial pressure and the left ventricle efflux fraction with ESR, evident of the tuberculosis intoxication. The most pronounced aggravation of the left ventricle function was recorded in the patients with the most severe tuberculosis process. The impairments in the left ventricle in the patients with active pulmonary tuberculosis were of functional nature. Due to intensive therapy of the tuberculosis, the indices of the left ventricle efflux function improved and the systemic arterial pressure came to normal, along with elimination of the tuberculosis intoxication signs.

The efficacy of cytoflavin as a supporting drug in the CAF chemotherapy of breast cancer was studied. It was estimated by the tolerability, the peripheric blood index dynamics, the patients general condition (Karnovsky Index) and the frequency of the side effects. For the immediate estimation of the polychemotherapy and cytoflavin effects on the cytoprotection natural system state, the dynamics of the glutathione metabolism and the lipid peroxidation in the erythrocytes was investigated.