Influence of Rimantadine, Ribavirine and Triazavirine on Influenza A Virus Replication in Human Monolayer and Lymphoblastoid Cell Lines
Abstract
The influence of antivirals, such as rimantadine, ribavirine and triazavirine on influenza virus replication in human cell cultures was evaluated. All the antivirals inhibited viral nucleoprotein NP synthesis. The strongest effect was shown for ribavirine in lung carcinoma A-549 cells and endothelial ECV-304 cells. Hoechst-33258 staining revealed induction of apoptosis in all the cell lines. Rimantadine and ribavirine inhibited virus-induced apoptosis while ribavirine enhanced it. The effect was registered in monolayer cell cultures as well as in suspension cell cultures. The influence of the antiviral drugs on the virus-induced cell proliferation in the suspension cell cultures is also described.
About the Authors
T. D. Smirnova
НИИ гриппа Минздравсоцразвития РФ
Russian Federation
Russian Federation
D. M. Danilenko
НИИ гриппа Минздравсоцразвития РФ
Russian Federation
Russian Federation
M. YU. Eropkin
НИИ гриппа Минздравсоцразвития РФ
Russian Federation
Russian Federation
E. G. Deeva
НИИ гриппа Минздравсоцразвития РФ
Russian Federation
Russian Federation
O. I. Kiselev
НИИ гриппа Минздравсоцразвития РФ
Russian Federation
Russian Federation
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Review
For citations:
Smirnova T.D., Danilenko D.M., Eropkin M.Yu., Deeva E.G., Kiselev O.I. Influence of Rimantadine, Ribavirine and Triazavirine on Influenza A Virus Replication in Human Monolayer and Lymphoblastoid Cell Lines. Antibiot Khimioter = Antibiotics and Chemotherapy. 2011;56(11-12):11-16. (In Russ.)
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