In Vitro Study of Antitumor and Antimicrobial Activity of a Preparation of Pegylated Liposomes with Sanguinarine
Abstract
Sanguinarin is a plant alkaloid with a wide spectrum of biological activity. The effectiveness of sanguinarin can be increased by its integration into liposomal nanoparticles. The aim of this study was to obtain and purify the pegylated liposome sanguinarine, to study its properties, as well as antitumor and antimicrobial activity in vitro. Pegylated liposome nanoparticles containing sanguinarinewere obtained. The size of the liposomes was 61.8+5.7 nm; the effectiveness of the inclusion of sanguinarine in liposomes was 82.3+4.9%. The article studies the release of sanguinarin from liposome particles and demonstrates its prolonged nature. An in vitro study showed that liposomal sanguinarine exhibited dose-dependent cytotoxic activity against tumor cells of MCF-7 (12.8 μM), L1210 (17.4 μM), A431 (18.67 μM) and HepG2 (20.7 μM). The antimicrobial effect of the liposome form of the drug was established for Gram-positive (B.subtilis ATC 6633 and B.coagulans 429) and Gram-negative (E.coli ATCC 8739) bacteria, as well as opportunistic fungi Aspergillus ustus 6K. The results indicate the prospects of further study of pegylated liposomal sanguinarine as an antitumor and antimicrobial agent.
About the Authors
S. V. Lutsenko
I. M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation
Russian Federation
Russian Federation
T. I. Gromovykh
I. M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation
Russian Federation
Russian Federation
V. V. Kashirin
I. M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation
Russian Federation
Russian Federation
V. N. Kuryakov
Oil and Gas Research Institute RAS
Russian Federation
Russian Federation
A. A. Baranova
Gause Institute of New Antibiotics
Russian Federation
Russian Federation
V. S. Sadykova
Gause Institute of New Antibiotics
Russian Federation
Russian Federation
N. B. Feldman
I. M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation
Russian Federation
Russian Federation
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Review
For citations:
Lutsenko S.V., Gromovykh T.I., Kashirin V.V., Kuryakov V.N., Baranova A.A., Sadykova V.S., Feldman N.B. In Vitro Study of Antitumor and Antimicrobial Activity of a Preparation of Pegylated Liposomes with Sanguinarine. Antibiot Khimioter = Antibiotics and Chemotherapy. 2018;63(3-4):3-7. (In Russ.)
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