The influence of polymyxin on the change in the electrophysical parameters of a suspension of Escherichia coli cells of the K-12 strain was studied for the first time by electroacoustic analysis. It was shown that the maximum changes in the detected signal occur at a concentration of polymyxin of 25 μg/ml, furthermore they did not depend on the time of exposure to the antibiotic. The data obtained by the acoustic analysis method was confirmed by the standard microbiological method for determining microorganisms' sensitivity to polymyxin. The article shows the possibility of recording the sensitivity of microbial cells to antibacterial drugs and determining their antibacterial activity by the example of polymyxin with the use of the method of electroacoustic analysis of cell suspensions.

Antibacterial activity of hybrid antibiotics vancomycin-azithromycin (C11, C12-carbonate) and eremomycin-azitromycin (C11, C12-carbonate) was evaluated. Quantum chemical calculations of complexes of hybrid antibiotics with a model tripeptide ligand a, ε-di-Ac-L-Lys-D-Ala-D-Ala by the semi empirical PM6 method provided data on geometrical parameters of complexes along with the energy of their formation and the influence of protonation of the NHCH₃ group. A correlation between the energy of formation of antibiotics-ligand complexes and antibacterial activity of hybrid antibiotics against Gram-positive bacterial strains was found.

The polymeric form ofjosamycin was developed by loading of the antibiotic into spherical submicron particles made of poly(DL- lactide-co-glycolide) (PLGA 50/50) by double emulsion method. The average diameter and zeta-potential of particles synthesized were less than 150 nm and -35 mV respectively. Polymeric form showed antimicrobial activity against gram-positive, gram-negative, and atypical bacteria. The highest antibacterial activity compared to substance (more than 8-fold) was detected against Enterococcus faecalis. The results of in vivo studies of specific activity of drugs on the model of staphylococcal sepsis in mice showed an increase in the efficacy of the studied polymeric form by 1.5-fold compared with the josamycin substance.

Results of the microbiological bench testing of antibacterial and antimycotic activity of the carbon sorbents modified with lactic acid oligomers are presented. It was found that the studied microorganisms - Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus agalactiae, Enterococcus faecalis, Pseudomonas aeruginosa, Streptococcus pyogenes, Klebsiella pneumonia, and Escherichia coli - are resistant to the majority of antimicrobial preparations that are widely used nowadays in clinical practice for treatment of bacterial and mycotic infectious diseases. Antimycotic effect of the modified sorbents toward yeast-like fungi Candida albicans was revealed. It was established for the first time that the modified sorbents have pronounced antimicrobial properties toward associative bacterial-fungal culture S.aureus + C.albicans, especially the carbon sorbent that was modified with a 50% lactic acid solution with subsequent polycondensation.

This article focuses on the experimental study ofreproductive safety of three sequential courses of Kagocel introduced to rats (males, females) during the period of impuberty (from the 10th day to the 45th day of life). The drug was administered in a therapeutic dose (TD) and at a dose exceeding that by 10 times (10TD). When the animals reached the mature age, the rats of the experimental groups were mated with intact partners and with each other. To assess reproductive safety, the fertility index and the embryonic death rates were determined. In the antenatal period of development the weight, the size of the fetuses, sex distribution, the status of their internal organs (Wilson method), and the state of the ossification processes (Dawson method) were determined on the 20th day of pregnancy. The survival index and body mass dynamics were evaluated in the postnatal period. The results of the study showed that all investigated parameters did not differ from those in the control group (placebo, P≤0.017). The received data testify to reproductive safety of prophylactic courses of Kagocel used during all period of impuberty.

The article highlights modern concepts of inflammatory diseases in adults and children in terms of genetic possibilities for diagnosing Crohn's disease and ulcerative colitis. It names the main candidate genes, which, in the presence of their specific polymorphisms, indicate the occurrence of a high risk of inflammatory bowel disease. The principles of modern therapy, the possibilities of pharma-cogenetic testing in predicting the effectiveness or risks of reducing the safety of drug therapy are described. The article focuses on modern trends and perspectives in ulcerative colitis and Crohn's disease treatment.

During the chronic urogenital chlamydial infection, violations of all links of pathogenesis, as well as changes in the composition and properties of biologically active substances, i.d. cytokines, including interferons, are noted. The authors suggest novel approaches, including cytokines, especially IFN-gamma, which is the most important in the formation of general immunity, including its combination with IFN-alpha, -beta, and lambda.

The article presents historical aspects of the treatment of cutaneous leishmaniasis. The literature data on the treatment of cutaneous leishmaniasis before the 30s of the last century testify to the attempts of chemotherapy of the disease by various means. There were entire groups of means and various treatment regimens in the arsenal of folk healers. This review highlights the treatment of cutaneous leishmaniasis, which existed in the territory of Imperial Russia and the former USSR from ancient times to the present.